Nikon A1 Instruction Manual page 66

22.6 Determine image acquisition conditions.
(4)-1 Live (Starting scanning)
(8) Acquisition
(1) Selecting
lasers
(3) Selecting
a pinhole
(6)-1 Selecting resolution
(1) Select the laser and channel to be used.
(2) If you want to acquire a transmitted image together with a spectral image, click the TD [IN] button and
check the TD checkbox.
Note: Before acquiring a transmitted image, turn off the light above the microscope.
(3) Select the laser wavelength to be used from the pull-down menu of [Pinhole].
Select a pinhole size best suited for the objective with [▲Home].
Chapter 22. Capturing Virtual Filter Images (Spectral Detector)
(2)-1 Selecting a transmitted image
(6)-3 Selecting
scan speed
(6)-2 Laser application
time per pixel
66
(4)-2 Adjusting laser power and
gain
(2)-2 Selecting a
transmitted image
A1