Nikon A1 Instruction Manual page 58

2) Spectral unmixing using reference data
(1) Select [Image] - [Spectral Unmixing Setting] from the menu bar.
(2) Select [All] from the [Category] list box.
(3) Select reagents to be used for spectral unmixing and click the [Add] button to add them to [Unmixing
Elements] (list on the right pane).
(4) Set pseudo-colors after spectral unmixing.
(5) Click the [Unmix] button to perform spectral unmixing.
(2)
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3) Spectral unmixing by creating reference data
(1) Save spectral data as reference data.
Click at the side of the image frame
and draw ROIs on the image using tools.
Note: Specify simple stain areas only.
Chapter 20. Separating Spectral Image (Unmixing)
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(4)
(5)