Nikon A1 Instruction Manual page 16

Confocal microscope
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(5) Click the
checking the image.
HV:
4ch detector sensitivity
Offset:
Signal cutoff (standard: 0)
Laser:
Laser power
Note: Use Offset "0" as the standard setting.
Note: Using the
[Pixel Saturation Indication] button in the LUTs tab during adjustment makes it
easy to adjust the sensitivity.
Note: If the displayed image is dark, click the
channel automatically to make the image clear.
Note: Turning on and off the [Live] button (scan ON/OFF) during adjustment minimizes fading.
Auto Scale All
Note: If the LUTs tab is not displayed, right-click the gray area of the software and select
[Visualization Control] - [LUTs] from the displayed menu to call it.
(6) To use Auto Gain, a function that automatically adjusts the detector sensitivity (HV) based on the set rate
of saturated pixels, click the
"NG" is displayed for channels that failed in Auto Gain and the HV values are returned to the previous
values. Use the
value and minimum value of the rate.
Notes:
Auto Gain is disabled during scanning.
Auto Gain is disabled during 2Ex1Em or 1Ex2Emx2 line sequence.
Auto Gain is disabled when line scan is set.
Do not make a manual adjustment in the Acquisition window and do not make an adjustment
using the remote controller during Auto Gain.
Chapter 4.
Capturing Color Images (Standard Detector)
[Live] button and adjust [Laser] (laser power) and [HV] (detector sensitivity) while
[AG] button.
[Auto Gain setting] button to change the rate of saturated pixels. Set the maximum
16
[Auto Scale All] button to adjust the contrast of the
Using the
[Pixel Saturation Indication] button during
adjustment colors the saturation region.
A1

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