Nikon A1 Instruction Manual page 31

9.4 Register laser power, HV, or others at each Z position.
(1) Check the [Move Z to selected Point]
checkbox and double-click the Z position in
the Z [μm] column to move the focus to the
displayed Z position.
(2) Check the [Use on Live] checkbox under [Live
Correction].
When this checkbox is checked, changing the
Z position displays the Live image with the set
HV and laser power.
After you finish checking, uncheck the
checkbox.
(3) After the Z position is moved to where you want to adjust brightness, adjust HV and laser power.
(4) To register the adjusted HV and laser power, click
After you finish adjusting brightness at each Z position, you can close [Z Intensity Correction].
(3)
(5) After you finish all registrations, click the [Run
Z Corr] button in the Capture Z-Series dialog
box to start image acquisition.
Chapter 9. Capturing Z Series Images (While Changing Brightness)
(1)
(2)
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(4)
A1