Nikon A1 Instruction Manual page 21

Chapter 5.
(6) To use Auto Gain, a function that automatically adjusts the detector sensitivity (HV) based on the set rate
of saturated pixels, click the
"NG" is displayed for channels that failed in Auto Gain and the HV values are returned to the previous
values.
Use the
and minimum value of the rate.
Notes:
• Auto Gain is disabled during scanning.
• Auto Gain is disabled during 2Ex1Em or 1Ex2Emx2 line sequence.
• Auto Gain is disabled when line scan is set.
• Do not make a manual adjustment in the Acquisition window and do not make an adjustment
using the remote controller during Auto Gain.
(7) Set the number of pixels to the necessary resolution. (e.g. 512 x 512)
If the image is dark, reduce the scan speed.
Note: Check the pixel dwell for when the resolution is changed.
Pixel dwell indicates laser application time per pixel.
The larger the value, the brighter the image that can be acquired.
(8) Apply Line Average as needed.
Average is a function to scan the same image
multiple times and average it to remove
noises.
Select [Line Average/Integrate] - [Average] in
the Scan setting window and select an
average scan frequency in [Count].
Note: Averaging reduces noise, but decreases
the frame rate (number of images
acquirable per second).
(9) Click the
5.4 Save an image.
Make the image you want to save active and select [File] - [Save As] to save it.
Note: We recommend that the image be saved in nd2 file format. Conditions such as parameters are
also saved.
Capturing Multistained Images: Cross Talk Reduction (Standard Detector)
[AG] button.
[Auto Gain setting] button to change the rate of saturated pixels. Set the maximum value
[XY] button to acquire an image.
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A1