Nikon A1 Instruction Manual page 53

19.5 Set the optical path. (Optical path setting for the confocal system required for
acquiring images)
Check the settings.
(1) Click
(2) Click the
(3) Click the
(4) Check the checkboxes of the channels of lasers to be used.
(5) Select a value for [Resolution] (wavelength resolution to be used) from "2.5 nm", "6 nm", and "10 nm" and a
value for [Channels] (number of channels to be used) from "1" to "32". Set the wavelength band to be acquired
by shifting the bar.
(6) Select "BS20/80" for [1st DM].
(7) If acquiring a transmitted image together with a spectral image, click
Note: Before acquiring a transmitted image, turn off the light above the microscope.
Note: Because the transmitted light detector is placed in front of transmitted light, transmitted images
(differential interferences (DIC)) cannot be observed visually while putting the transmitted light
detector into the optical path.
To observe transmitted images visually, remove the transmitted light detector from the optical path.
Chapter 19. Capturing Spectral Images (Spectral Detector)
to open the Optical path window.
[SD] button to select the spectral detector.
[Manual] button to set the optical path in the manual mode.
(4)
(6)
(7)
(1)
(5)-1
(3)
(2)
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to bring into the optical path.
(5)-2
A1