Nikon A1 Instruction Manual page 80

Confocal microscope

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Chapter 25. Capturing High-Speed Images (Resonant Scanner)

(2) Select the laser and channel to be used.

(3) If you want to acquire a transmitted image together with a confocal image, click the TD [IN] button and

check the TD checkbox.

Note: Before acquiring a transmitted image, turn off the light above the microscope.

(4) Select the laser wavelength to be used from [Pinhole].

Select a pinhole size best suited for the objective with [▲Home].

(5) Click the

checking the image.

Note: Use Offset "0" as the standard setting.

Note: Using the

[Pixel Saturation Indication] button in the LUTs tab during adjustment makes it

easy to adjust the sensitivity.

Note: If the displayed image is dark, click the

channel automatically to make the image clear.

Note: Turning on and off the [Live] button (scan ON/OFF) during adjustment minimizes fading.

Auto Scale All

Note: If the LUTs tab is not displayed, right-click the gray area of the software and select

[Visualization Control] - [LUTs] from the displayed menu to call it.

[Live] button and adjust [Laser] (laser power) and [HV] (detector sensitivity) while

[Auto Scale All] button to adjust the contrast of the

Using the

[Pixel Saturation Indication] button during

adjustment colors the saturation region.

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