Nikon A1 Instruction Manual page 37

Confocal microscope
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12.3
Acquire time series images.
(1) Set the number of pixels to the necessary resolution. (e.g. 512 x 512)
If the image is dark, reduce the scan speed.
Note: Check the pixel dwell for when the resolution is changed.
Pixel dwell indicates laser application time per pixel.
The larger the value, the brighter the image that can be acquired.
(2) Apply Line Average as needed.
Average is a function to scan the same image
multiple times and average it to remove
noises.
Select [Line Average/Integrate] - [Average] in
the Scan setting window and select an
average scan frequency in [Count].
Note: Averaging reduces noise, but decreases
the frame rate (number of images
acquirable per second).
(3) Click the
acquire a time series image.
Chapter 12. Capturing Time Series Images
[Run now] button to
37
A1

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