Nikon A1 Instruction Manual page 11

Confocal microscope
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3.6
Perfect Focus System Operation
(1) Check that the dichroic mirror IN/OUT lever is
set to IN.
(2) Set the objective to be used in the optical path
and adjust the focus with the focus knob on
the microscope to focus on the sample.
(3) Press the PFS-ON button on the front
operation panel.
(When the PFS starts, the PFS-ON indicator
lights green.)
(4) Focus on the position at which focus is maintained with the offset dial on the PFS offset controller and
start microscopy.
Once the PFS operation is started, focusing with the focus knob on the microscope is disabled.
Registering offset amount: The focus position adjusted with the offset dial can be registered with the
Recalling offset amount:
BRIGHTNESS
FOCUS
EYE
ON
L100
L80
Chapter 3.
(with the Front Operation Panel)
PFS-MEMORY button.
Registered focus position can be called with the PFS-RECALL button.
Z - RESET
DISPLAY
RECALL
MEMORY
PFS
R100
1X
Figure
Ti-E Front Operation Panel and Offset Controller
Operation of the Microscope
Dichroic mirror IN/OUT lever
PFS-ON
button/indicator
PFS-MEMORY
button/indicator
PFS-RECALL
button/indicator
FINE/COARSE selector
button
11
A1

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