Nikon A1 Instruction Manual page 55

Chapter 19. Capturing Spectral Images (Spectral Detector)
(1) If you want to acquire a transmitted image together with a spectral image, click the TD [IN] button and
check the TD checkbox.
Note: Before acquiring a transmitted image, turn off the light above the microscope.
(2) Select the laser wavelength to be used from [Pinhole].
Select a pinhole size best suited for the objective with [▲Home].
(3) Click the
checking the image.
Note: The Si HV setting is common to all lasers. Make fine adjustments with the laser power setting.
Note: Using the [Pixel Saturation Indication] button in the LUTs tab during adjustment makes it
easy to adjust the sensitivity.
Note: If the displayed image is dark, click the
channel automatically to make the image clear.
Note: Turning on and off the [Live] button (scan ON/OFF) during adjustment minimizes fading.
Auto Scale All
Note: If the LUTs tab is not displayed, right-click the gray area of the software and select
[Visualization Control] - [LUTs] from the displayed menu to call it.
(4) To use Auto Gain, a function that automatically adjusts the detector sensitivity (HV) based on the set rate
of saturated pixels, click the
"NG" is displayed for channels that failed in Auto Gain and the HV values are returned to the previous
values.
Use the [Auto Gain setting] button to change the rate of saturated pixels. Set the maximum value
and minimum value of the rate.
Notes:
• Auto Gain is disabled during scanning.
• Auto Gain is disabled when line scan is set.
• Do not make a manual adjustment in the Acquisition window and do not make an adjustment
using the remote controller during Auto Gain.
[Live] button and adjust [Laser] (laser power) and [Si HV] (detector sensitivity) while
[AG] button.
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[Auto Scale All] button to adjust the contrast of the
Using the [Pixel Saturation Indication] button during
adjustment colors the saturation region.
A1