Nikon A1 Instruction Manual page 60

(5) Select [Image] - [Spectral Unmixing Setting] from the menu bar.
(6) Select [User Defined] from the [Category] list box.
(7) Select reagents (spectral data saved in step 4) to be used for spectral unmixing and click the [Add] button
to add them to [Unmixing Elements] (list on the right pane).
(8) Set pseudo-colors after spectral unmixing.
(9) Click the [Unmix] button to perform spectral unmixing.
(6)
(7)-1
How to use Spectrum Profile
Displays the Y axis of graph as
relative values.
Displays the Y axis of graph as
brightness values.
Inserts a spectrum graph originated by
the fluorescence manufacturer
as reference of analysis.
Chapter 20. Separating Spectral Image (Unmixing)
(7)-2
60
(8)
(9)
A1