To Deconvolute A Mass Spectrum - Agilent Technologies 6100 Series LC/MS Quick Start Manual

Deconvolution
Deconvolution is an optional process (additional license required) that
transforms mass spectra from multiply-charged ions into a calculated
molecular weight.

To deconvolute a mass spectrum

Instructions
1 In the Data Analysis view, load an electrospray MS
2 Generate the MS spectrum that you want to
3 Click the Enter Deconvolution Tool button to
4 When the spectrum is displayed, click the
5 Examine the ions that were found. Very noisy data or
6 Click the Edit Deconvolution Parameters
7 Click the Run Deconvolution button to begin
Agilent 6100 Series LC/MS System Quick Start Guide
data file. The TIC is displayed.
deconvolute. (Select the Spectral task tool set and use
the spectrum selection tools to pick the spectrum of
interest).
set up the deconvolution display area.
Label Ions button to locate masses to be
used in deconvolution.
data with unresolved regions often need special
settings to find ions.
button to change any of the parameters.
the deconvolution process.
Deconvolution
To deconvolute a mass spectrum
Comments
These operations are performed on
data acquired in full scan mode.
You can use the Ion Label
Options button to optimize
the set of ions found.
Once the deconvolution is done, the
components are displayed in the
upper right window. The charge states
are displayed in the lower right
window.
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