Agilent Technologies 6100 Series LC/MS Quick Start Manual page 20

Basic Operation
Tune the LC/MS
Task
To use Check Tune
To use Manual Tune
20
Instructions
1 Select Check Tune from the Tune
menu.
2 Review the Check Tune report.
Adjustments are suggested for values
that are outside of acceptable ranges.
1 Select Manual Tune from the Tune
menu.
2 Set the desired mass resolution
(adjusting width gain and width
offset).
3 Calibrate the mass axis (adjusting
mass gain and mass offset).
4 Optimize the ion transmission through
the source ion optics (fragmentor,
skimmer(s), lens 1, lens 2, and
octopole peak).
5 Adjust the signal strength (setting iris
and adjusting the multiplier gain).
Fragmentor and gain are method
parameters. The fragmentor affects ion
transmission and fragmentation. For
more information, see the online Help.
Agilent 6100 Series LC/MS System Quick Start Guide
Comments
Use Check Tune to determine quickly
whether the LC/MS is correctly tuned.
It performs a single profile scan of the
tune masses and compares the peak
widths and mass axes with target
values.
Use Manual Tune when you want to:
• Achieve maximum sensitivity by
sacrificing some resolution
• Tune specifically for the very low
end (<150 amu) of the mass range
• Tune with a compound other than
the standard calibrants