Basic Operation; Start Up And Shut Down The System - Agilent Technologies 6100 Series LC/MS Quick Start Manual

Basic Operation

Start up and shut down the system

Basic Operation
Start up and shut down the system
Task
To start up the system
To put the system in Standby Mode
18
Instruction
1 From the Method and Run Control
view, click the button on the
system status bar. When you move
mouse over the button, if system is
currently idle, it turns green.
2 Set up the LC conditions (pump,
column heater, and detector).
3 Set up the LC/MS spray chamber
conditions as described in the MSD
Spray Chamber dialog box topic in
the online Help.
Allow 15 minutes for the system to warm
up.
1 Flush the system with pure mobile
phase to ensure that the flow path is
clear of buffers. This prevents the
nebulizer from plugging. A plugged
nebulizer can cause a high back
pressure, which can damage the LC
flow cell.
It is good practice to flush the flow
path (including the column) for 5 to 10
minutes with a mobile phase without
buffers such as 50:50
water/acetonitrile.
2 Click the button on the system
status bar. All modules are set to
standby mode (indicated by the gray
color in the system diagram).
Agilent 6100 Series LC/MS System Quick Start Guide
Comment
This procedure assumes that the
system is under vacuum and the LC
and LC/MS are properly connected. It
also assumes that the liquid flow path
is properly set up.
Put the system in standby mode
overnight or whenever you are not
analyzing samples for an extended
time. The standby state for the LC/MS
leaves the nebulizer and drying gas on
at low flow.
Standby for the LC/MS is:
• 20 psi for the nebulizer,
• 3 L/min for drying gas,
• 300 °C for drying gas temperature,
• 325 °C for the APCI vaporizer, if
present
• the MS stream selection valve is
set LC to Waste.
For Agilent Jet Stream source:
• Sheath temp 100°C
• Sheath gas flow 2.2 L/minute