Operating Tips - Agilent Technologies 6100 Series LC/MS Quick Start Manual

Operating Tips

• Back up your data and methods regularly to avoid loss of data in case the
• Put the system in standby mode overnight or whenever you are analyzing
• Verify that the tune file you are using is appropriate for your samples.
• Save Tune reports in an MS Logbook for future reference.
• Regular system maintenance can reduce problems. Keep a maintenance
• Use the Maintenance Logbook and EMF features in Diagnosis view to help
• Flush the sample path and clean the spray chamber, capillary tip, and spray
• The spray chamber vent hose must be connected to a lab vent that is used
• Samples must be filtered. If no chromatography is used, they must be salt
• If a UV detector is available, use it in series with the LC/MS. Try to
• To avoid chromatographic band broadening, be sure that all tubing
• Use the following table as a guide to using SIM, condensed scan, and full
Task
Acquire electrospray data for samples containing large,
multiply-charged analytes.
Analyze a mixture with unknown components (small molecules).
Agilent 6100 Series LC/MS System Quick Start Guide
files are accidentally overwritten, deleted, or if a hardware problem
develops with your disk drive.
samples for an extended time.
record.
you track when maintenance is needed and to keep an online maintenance
record.
shield daily or at the end of each shift. Check the foreline pump fluid level
every week.
only for the source. The vent hose must be separate from the vent hose for
the foreline pump. Otherwise, waste products can migrate into the spray
chamber vent producing chemical noise.
and detergent-free.
minimize chromatographic peak broadening by using low dispersion tubing.
connections are free of dead volume. Use zero dead-volume (ZDV) fittings
when possible.
scan acquisition modes.
Operating Tips
Mode
Full Scan
Scan
25