Agilent Technologies 6100 Series LC/MS Quick Start Manual page 29

Unstable signal
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High spectral noise
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Droplets, not spray, exiting the nebulizer
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Agilent 6100 Series LC/MS System Quick Start Guide
Verify that the drying gas flow and temperature are correct for the solvent
flow you are using.
Verify that the solvent is thoroughly degassed. Do not use ultrasonic
degassing with protein samples.
Verify that the LC backpressure is steady; steady backpressure indicates a
steady solvent flow.
Use appropriate mass filter values.
Check the spray shape. Nebulizer can be damaged or incorrectly set.
Verify that drying gas flow and temperature are correct for the solvent flow
you are using.
Verify that the solvent is thoroughly degassed. Do not use ultrasonic
degassing with protein samples.
Verify that the LC backpressure is steady; steady backpressure indicates a
steady solvent flow.
If you are using water as part of the mobile phase, verify that it is deionized
(>18M).
Verify that the nebulizing gas pressure is set high enough for the LC flow
being used.
Check the position of the needle in the nebulizer.
Stop the solvent flow and remove the nebulizer assembly. Use a magnifying
glass to examine the end of the nebulizer for damage.
Troubleshooting Tips
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