Troubleshooting Tips - Agilent Technologies 6100 Series LC/MS Quick Start Manual

Troubleshooting Tips

No peaks
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Poor mass accuracy
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Low signal
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Agilent 6100 Series LC/MS System Quick Start Guide
Verify that there is spray from the nebulizer.
Verify that the capillary voltage is set correctly.
In APCI mode, verify that the corona needle is installed.
In APCI mode, verify that the Corona Current setting is 2 μA or higher, and
that the Corona Voltage is between 1500 and 5000 V.
Verify that the MS system is tuned correctly.
Verify that MS vacuum pressures are within normal ranges.
Check the drying gas flow and temperature.
Verify that the fragmentor is set correctly.
Check if any error message indicates a problem with electronics.
Verify that sufficient sample is present in vial.
Recalibrate the mass axis.
Verify that the ions used for tuning span the mass range of the sample ions
and that they show strong, stable signals.
Check the solution chemistry. Verify that the solvent you are using is
appropriate for your sample. Mixed samples can exhibit signal suppression
of one or more components.
Verify that the sample is fresh and has been stored correctly.
Verify that the MS system is tuned correctly.
Check the nebulizer condition.
Clean the capillary entrance.
Check the capillary for damage and contamination.
Troubleshooting Tips
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