Beckman Coulter Microfuge 18 Instruction Manual
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Beckman Coulter Microfuge 18 Instruction Manual

Beckman Coulter Microfuge 18 Instruction Manual

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MM-IM-10
®
Microfuge
®
18 Centrifuge
Instruction Manual

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Summary of Contents for Beckman Coulter Microfuge 18

  • Page 1 MM-IM-10 ® Microfuge ® 18 Centrifuge Instruction Manual...
  • Page 2 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)

  • Page 3: Safety Notice

    SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual. The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation, operation, mainte- nance, or repair of this centrifuge.
  • Page 4 Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter.
  • Page 5 MM-IM-10 August 2009 Microfuge 18 Centrifuge ® Instruction Manual © 2009 Beckman Coulter, Inc.

  • Page 7: Table Of Contents

    Contents Page INTRODUCTION Certification ..........Scope of Manual.
  • Page 8 Figure 1-1. The Microfuge 18 Centrifuge ....... 1-2 Figure 1-2.

  • Page 9: Introduction

    Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com. SCOPE OF MANUAL This manual is designed to familiarize you with the Beckman Coulter Microfuge 18 centrifuge, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read...

  • Page 10: Conventions

    Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

  • Page 11: Cfc-Free Centrifugation

    CFC-FREE CENTRIFUGATION To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Microfuge 18 centrifuges. RADIO INTERFERENCE This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules. These...

  • Page 12: Canadian Regulations

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

  • Page 13: Description

    Description CENTRIFUGE FUNCTION, SPECIFICATIONS, AND SAFETY FEATURES CENTRIFUGE FUNCTION The Beckman Coulter Microfuge 18 (Figure 1-1) is a microprocessor- controlled compact benchtop centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter F241.5P rotor, designed specifically for use in this centrifuge, applications include: •...

  • Page 14: Specifications

    Pollution degree..........2 The Microfuge 18 centrifuge can be used in a humidity controlled cold room.

  • Page 15: Available Rotors

    Lid material ........polycarbonate SAFETY FEATURES The Microfuge 18 centrifuge has been designed and tested to operate safely indoors at altitudes up to 2 000 m (6 562 ft). Safety features include the following.

  • Page 16: Ground Wire Check

    Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge. CHASSIS HOUSING The housing is a sheet-steel casing. The control panel is covered by a protective overlay made of coated polycarbonate.

  • Page 17: Drive

    Description DRIVE The asynchronous direct-drive motor is brushless for low mainte- nance. A tie-down screw secures the rotor to the drive shaft. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation.
  • Page 18 Description SPEED The RPM knob is used to select the run speed, up to 14 000 rpm, in 500-rpm increments. The entered speed is indicated by an LCD light bar on the display. The upper scale of the display shows the speed in rpm (revolutions per minute);...
  • Page 19 Description TIME Using the TIME knob, operation in any of three modes can be selected: • A timed run can be set for any run time up to 30 minutes in 1-minute increments. Lighted bars count down time remaining and “min”...
  • Page 20 Description • An alternate pulse-style run can be selected with the timer set to the short run position. When the START key is pressed and released, the centrifuge will accelerate to the selected speed and run for 30 seconds, then decelerate to stop. Lighted bars count elapsed run time in 1-second increments, and “sec”...
  • Page 21 Description • Pressing the STOP key a second time (before pressing START) will select slow deceleration only. The words SOFT STOP will show on the display, and deceleration time will be doubled. x1000rpm short 8 10 12 14 10 14 18 soft stop x1000rcf •...

  • Page 23: Installation

    Installation INSTALLING THE CENTRIFUGE WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge’s air system and be ignited by the motor. No hazardous materials should be handled or stored within the 30-cm (1-ft) boundary surrounding the centrifuge.

  • Page 24: Electrical Requirements

    15.6 39.5 24.5 12.6 32.0 24.0 Figure 2-1. Dimensions of the Microfuge 18 Centrifuge • Ambient temperatures during operation should not be lower than 4°C (39°F) or higher than 35°C (95°F). Relative humidity should not exceed 80% (noncondensing). ➠ NOTE...

  • Page 25: Test Run

    Installation To reduce the risk of electrical shock, this centrifuge uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge.

  • Page 27: Operation

    Operation WARNING Handle infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills, operator error, or tube failure may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions.

  • Page 28: Run Procedure

    Operation RUN PROCEDURE CAUTION The Microfuge 18 centrifuge and F241.5P rotor were developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Safety or reliability of the centrifuge cannot be assured if used with any other rotor. Use of any equipment not specifically recommended in this manual is the sole responsibility of the user.
  • Page 29 Operation Action Result 3. Install the rotor according to instructions in the rotor manual. Always run the rotor with a balanced load. 4. Secure the rotor to the drive shaft with the tie-down screw by holding the rotor with one hand while turning the T-handle rotor wrench to the right (clockwise).

  • Page 30: Starting A Timed Or Hold Run

    Operation STARTING A TIMED OR HOLD RUN Action Result 1. Turn the RPM knob to the x1000rpm selected speed (up to short 8 10 12 14 14 000 rpm). 10 14 18 x1000rcf 2. Turn the TIME knob to the x1000rpm required run time (up to short...
  • Page 31 Operation Action Result • During a run, when the STOP key is lit, you can press it to termi- nate the run. During deceleration, you can press it again to select the slow acceleration and slow deceleration feature. The word SOFT will show on the display.

  • Page 32: Starting A Pulse Run

    Operation Action Result WARNING Do not attempt to override the door interlock system while the rotor is spinning. A timed run ends automatically when the set time counts down to zero. You can end a hold or timed run in progress for any reason by pressing the STOP key.
  • Page 33 Operation Action Result 3. Press and hold the The centrifuge accelerates to the maximum speed and continues as START key. long as the key is pressed, decelerating when the key is released. Lighted bars count elapsed run time in 1-second increments, and “sec”...

  • Page 34: Unloading

    Operation Action Result 3. Check that the door is shut and securely latched. 4. Press and release the The centrifuge accelerates to the selected speed and continues for START key. 30 seconds, then decelerates to stop. Lighted bars count elapsed run time in 1-second increments, and “sec”...

  • Page 35: Troubleshooting

    Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call your local Beckman Coulter representative. To help diagnose and correct the problem, try to gather as much infor- mation about the situation as you can: •...

  • Page 36: Error Messages

    Refer to Table 4-2 to determine the nature of the problem and recommended actions. If you are unable to correct the problem, call your Beckman Coulter Field Service representative. This would indicate Error Number 3.
  • Page 37 Troubleshooting Table 4-2. Error Message Chart. If the recommended action does not correct the problem, call Beckman Coulter Field Service. Error Number Description Recommended Action Bad tachometer reading Turn the power off (O) and wait for the rotor to come to a complete stop, then turn the power back on (I) to reset.
  • Page 38 Troubleshooting Action Result 2. Make sure that the rotor is No sound or vibration comes from the centrifuge. not spinning. 3. Tilt the centrifuge back and locate the emergency access device at the bottom front of the front panel. Do not Grasp pull here here...

  • Page 39: Care And Maintenance

    For maintenance not covered in this manual, contact Beckman Coulter Field Service (1-800-742-2345 in the U.S.A.; customers out- side the United States should contact their local Beckman Coulter representative). Refer to the rotor manual and Chemical Resistances (publication IN-175) for instructions on the care of rotors and rotor accessories.

  • Page 40: Maintenance

    Care and Maintenance MAINTENANCE PREVENTIVE MAINTENANCE Perform the following procedures regularly to ensure satisfactory performance and long service life of the centrifuge. • Regularly inspect the interior of the rotor chamber for accumula- tions of sample or dust. Clean as required (see , below), CLEANING as these accumulations can result in rotor vibrations.
  • Page 41 Care and Maintenance Action Result 2. Use your fingernails or a Latches release and the fuse holder can be pulled out. small screwdriver to press in on the fuse holder latches. 3. Pull the fuse holder Fuses are accessible. straight out. 4.

  • Page 42: Cleaning

    Resistances for more information regarding chemical resistance of centrifuge and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 43: Storage And Transport

    Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions.

  • Page 44: Replacement Parts

    Care and Maintenance ordering rotors, tubes, and accessories. For your convenience, a partial list is given below. Refer to the rotor manual for materials and supplies needed for rotors. REPLACEMENT PARTS F241.5P rotor..........367187 Rotor tie-down screw .
  • Page 45 Beckman Coulter terms portation charges collect unless the product is found to be and conditions in effect at the time of sale, Beckman Coulter defective, in which case Beckman Coulter will pay all trans- agrees to correct either by repair or, at its election, by replace- portation charges.
  • Page 51 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com ©2009 Beckman Coulter, Inc. All rights reserved...

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