Related Manuals for Beckman Coulter 45 Ti Rotor
Summary of Contents for Beckman Coulter 45 Ti Rotor
- Page 1 L5-TB-036VA February 2014 Type 45 Ti Rotor Used In Beckman Coulter Class H, R, and S Preparative Ultracentrifuges © 2014 Beckman Coulter, Inc.
- Page 2 Type 45 Ti Rotor Trademarks Beckman Coulter, the stylized logo, and Quick-Seal are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trade- marks or registered trademarks of their respective holders.
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Page 3: Safety Notice
Although rotor components and accessories made by other manufacturers may fit in the Type 45 Ti rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the Type 45 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. -
Page 4: Specifications
Type 45 Ti Rotor TYPE 45 Ti ROTOR 24° r min r av r max Axis of Rotation SPECIFICATIONS Maximum speed ........45 000 rpm Density rating at maximum speed. -
Page 5: Preparation And Use
See the Warranty at the back of this manual for warranty information. PREPARATION AND USE Specific information about the Type 45 Ti rotor is given here. Infor- mation common to this and other rotors is contained in Rotors and Tubes for Preparative Ultracentrifuges (publication LR-IM) which accompanies this manual. -
Page 6: Prerun Safety Checks
NOTE Although rotor components and accessories made by other manufacturers may fit in the Type 45 Ti rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the Type 45 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. - Page 7 Type 45 Ti Rotor 2. Load the filled containers symmetrically into the rotor. (Refer on page 8 for information about TUBES AND BOTTLES containers.) If fewer than six tubes are being run, they must be arranged symmetrically in the rotor (see Figure 1). Opposing tubes must be filled to the same level with liquid of the same density.
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Page 8: Operation
(361668) TUBES AND BOTTLES The Type 45 Ti rotor uses the tubes and bottles listed in Table 1. Be sure to use only those items listed, and to observe the maximum speed limits and fill volumes shown. (Maximum fill volume is the maximum amount that can be centrifuged in the container listed.) - Page 9 Type 45 Ti Rotor Table 1. Available Tubes and Bottles for the Type 45 Ti Rotor. Use only the items listed here. Tube Required Accessory Max Speed/ Part Part RCF/ k Factor Dimensions Description Number Fill Vol Description Number 38 × 102 mm...
- Page 10 Type 45 Ti Rotor Table 1. Available Tubes and Bottles for the Type 45 Ti Rotor (continued) Tube Required Accessory Max Speed/ Part Part RCF/ k Factor Dimensions Description Number Fill Vol Description Number 16 × 76 mm thickwall 355630 13.5 mL...
- Page 11 Type 45 Ti Rotor Temperature Limits • Plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and 25°C. For centrifugation at other temperatures, pretest tubes under anticipated run conditions. • If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior to centrifugation.
- Page 12 Type 45 Ti Rotor Tube Caps Thinwall tubes require caps for tube support during centrifugation; thickwall tubes and bottles can be run with or without caps (speed O-Ring reduction may be required). Use only the caps listed in Table 1. Refer Gasket to Rotors and Tubes for the use, assembly, and maintenance of caps.
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Page 13: Run Times
The k factor of the rotor is a measure of the rotor’s pelleting effi- TIME HR:MIN ciency. (Beckman Coulter has calculated the k factors for all of its preparative rotors at maximum rated speed and using full tubes.) The k factor is calculated from the formula: ⁄... -
Page 14: Run Speeds
Type 45 Ti Rotor Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows: ⎛ 45 000 ⎞ -------------------------------------- - ⎝ ⎠ actual run speed Run times can also be estimated from data established in prior experi- ments using a different rotor if the k factor of the previous rotor is known. - Page 15 10 000 11 600 7 810 4 010 2 689 *Calculated for all Beckman Coulter preparative rotors as a measure of the rotor’s relative pelleting efficiency in water at 20°C. Relative Centrifugal Fields Type 45 Ti Rotor 250 000 200 000...
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Page 16: Selecting Cscl Gradients
The reference curves in Figure 3 show gradient distribution at equi- librium. Each curve in Figure 3 is within the density limits allowed for the Type 45 Ti rotor: each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities (one for each fill level) that avoid precipitation at that speed. - Page 17 1.10 Rotor Speed (K rpm) Figure 2. Precipitation Curves for the Type 45 Ti Rotor. Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation. The dashed line is a representation of equation (6), and is shown here to illustrate the...
- Page 18 1 /2-filled 1 /4-filled Distance from Axis of Rotation (mm) Figure 3. CsCl Gradients at Equilibrium for the Type 45 Ti Rotor. Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds (from Figure 2) results in gradients presented here.
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Page 19: Adjusting Fill Volumes
Type 45 Ti Rotor ADJUSTING FILL VOLUMES Figures 2 and 3 show that several fill volumes are possible in a tube. If a tube is partially filled with gradient solution, float mineral oil (or some other low-density, immiscible liquid) on top of the tube contents to fill the tube to its maximum volume. -
Page 20: Care And Maintenance
Type 45 Ti Rotor rotor and held upright (vertical and stationary), there will be about Upright 9 mm of centerband-to-centerband separation. This interband distance, d up , can be calculated from the formula: θ ----------- - θ where d is the interband distance when the tube is held at an angle, θ... - Page 21 Rotors and Tubes. Refer to the chemical resistances table in Appendix A of Rotors and Tubes for chemical compatibilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. CLEANING Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred.
- Page 22 Use it only when necessary and remove it promptly from surfaces. While Beckman Coulter has tested these methods and found that they do not damage components, no guarantee of sterility or disinfection is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.
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Page 23: Sterilization And Disinfection
Use the minimum immersion time for each solution, per laboratory standards. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfec- tion is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 24: Returning A Rotor
Before returning a rotor or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form may be obtained from your local Beckman Coulter sales office, and should contain the following information: •... -
Page 25: Supply List
REPLACEMENT ROTOR PARTS Type 45 Ti rotor assembly ....... . . 339160 Rotor lid. - Page 26 Type 45 Ti Rotor...
- Page 27 Preparative Ultracentrifuge Rotors ..5 years — No Proration 2) If a Beckman Coulter centrifuge is damaged due to a failure of Analytical Ultracentrifuge Rotors..5 years — No Proration...
- Page 28 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 U.S.A. Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com • Printed in U.S.A. © 2014 Beckman Coulter, Inc. All rights reserved...
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