Fluorescence Capture - GE Amersham Imager 680 Operating Instructions Manual

5.5.4

Fluorescence capture

Introduction
Fluorescence image capturing is performed with Epi-illumination (Blue, Green and Red)
or Trans-UV illumination. The Epi-illumination method allows for both single and multi-
channel detection. Fluorescence capturing is performed using a light source and emission
filter.
Note:
The methods available in Amersham Imager 680 configurations are described in the
following table:
Epi-RGB
Custom
Trans-UV
Note:
Fluorescence capture options
The table below illustrates sample types for fluorescence capturing:
Sample type
Gel (e.g. Cy™2/Cy3/Cy5)
Membrane (e.g. ECL Plex™
Cy2, ECL Plex Cy3, ECL
Plex Cy5)
Gel (e.g. EtBr/SYBR™
Green)
Amersham Imager 680 Operating Instructions 29271308 AB
Fluorescence capture is only available with Lower tray position.
680 680 UV 680 QC
x x
The Custom method allows for different combinations of the blue, green or
red light sources and an emission filter of choice.
Tray and accessory
(placement only in the
lower tray position)
Black Tray
Black Tray
UV Trans Tray (transpar-
ent) + gel sheet
5.5.4 Fluorescence capture
680 RGB
x
x
x
Light source (location in-
side)
Epi-Blue, Green, and Red
fluorescence (top)
Epi-Blue, Green, and Red
fluorescence (top)
Trans-UV (bottom)
5 Operation
5.5 Capture an image
141