GE Amersham Imager 680 Operating Instructions Manual page 142

5 Operation
5.5 Capture an image
5.5.4 Fluorescence capture
Fluorescence exposure options
There are four exposure options for fluorescence capturing. In the following table the
benefits of each exposure option are described.
When...
Optimal dynamic range
is required
A specific area of a
sample is of interest
The intensity of the im-
age is inadequate after
automatic exposure or
a known exposure time
is required
142
Then...
Select Auto for automatic exposure.
A short pre-exposure is performed to determine the signal
intensity. The system will use this information to calculate
which exposure time will give the highest possible signal
below saturation to enable accurate quantification of the
sample.
To use the full dynamic range of the image file, check High
dynamic range.
To minimize exposure time, leave High dynamic range
unchecked.
Select Semi-automatic.
A short pre-exposure is performed which results in an
image. This allows you to select the area of the sample
image to focus on. The software automatically calculates
the exposure time for the specified area.
To use the full dynamic range of the image file, check High
dynamic range.
To minimize exposure time, leave High dynamic range
unchecked.
Note:
Semi-automatic exposure is not available for multichannel
fluorescence capture.
Select Manual exposure and set the exposure time man-
ually.
Amersham Imager 680 Operating Instructions 29271308 AB