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Getting Started
• At least one blank well is required. If all blank wells are masked, the
Status
• Each masked blank well will be flagged with an asterisk in the printed and
exported results.
When the read is finished, the results are displayed and ready for output
5.
according to the settings under
Gen5 Software
Gen5 software supports all Epoch 2 reader models. This section provides brief instructions
for creating a protocol and running an experiment. For more information, refer to
publications provided with Gen5 and the Gen5 help system
In Gen5, a protocol contains instructions for controlling the reader and (optionally)
instructions for analyzing the data retrieved from the reader. At a minimum, a protocol
must specify the procedure you wish to run. After creating a protocol, create an
experiment that references the protocol. You will run the experiment to read plates and
analyze the data.
In the
Gen5 Task
1.
Open the
2.
click OK.
Select a
Plate
3.
Gen5 stores measurements and other characteristics for individual plate types in a
database. It is essential that you select (or define) the plate type to match the assay
plate. Otherwise, results may be invalid. See the
Gen5 Help for instructions.
Add steps to the procedure for reading the plate. Click
4.
reader supports the defined steps, and then click OK.
Optionally, perform any of these steps to analyze and report the results:
5.
• Open the
and/or standards to the plate.
• Open the
include Transformation,
Analysis.
• Create a report or export template via the
File > Save
Select
6.
In the
Gen5 Task
7.
protocol.
Select the desired protocol and click OK.
8.
Agilent BioTek Epoch 2 Instructions for Use
1771011I Revision C
is INVALID.
Manager, select
Procedure
dialog. If prompted to select a reader, select
Type.
Plate Layout
dialog and assign blanks, samples, controls,
Data Reduction
and give the protocol an identifying name.
Manager, select
Instrument >
Output.
(Help > Help
Protocol > Create
New.
"Plate Type
dialog to add data reduction steps. Categories
Well
Analysis,
Curve
Report/Export
Experiment > Create using an existing
Page 38 of 51
CV
Topics).
Epoch 2
Database" topic in the
Validate
to verify that the
Analysis, and
Qualitative
Builders.
Agilent Technologies, Inc.
and
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