Run A Take3 Protocol - Agilent Technologies BioTek Epoch 2 Instructions For Use Manual

Absorbance microplate spectrophotometer
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Getting Started
When finished, tap
6.

Run a Take3 Protocol

On the Main Menu, tap the
1.
Select 1 to 12 wells as
2.
On the
Run Protocol
3.
Concentration
Secondary Ratio WL
Spectral
nm step size), in addition to the endpoint reads.
Place the
4.
• If the
Take3 Blank Masking
DIVERGING, the
CV%
defined for the plate. Invalid blank wells are highlighted in red. Tap a
well to mask (exclude) it from blank average and
the remaining unmasked blank wells are valid, the
illuminates. If the number of valid blank wells is sufficient for the assay, tap
Approve, otherwise select
slides).
Agilent BioTek Epoch 2 Instructions for Use
1771011I Revision C
Back
Home
and
Take3
Blank
and tap Continue.
screen, select options as applicable:
units: Select the unit of measure for the export file.
(230 nm): Available for nucleic acid protocols.
Scan: Perform a spectral scan for the wavelength range displayed (1
Take3
plate on the carrier and tap Start.
%CV
of the blank wells is higher than the Blank threshold
to return to the
tab and select the protocol to run.
screen appears and reports that the
Cancel
and address the problem (e.g., clean the
Page 37 of 51
Main
Menu.
.
CV Status
%CV
calculations. When
Approve
button
Agilent Techologies, Inc.
is

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