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Getting Started
Endpoint Protocols
Plate: Options include standard 6-, 12-, 24-, 48-, 96-, and 384-well plates,
(if equipped), and
Primary WL
secondary wavelength in the range 200 to 999 nm.
Microplates and
Read
Speed: Select
See the Specifications section to compare the performance and timing
specifications.
Blanks: Tap to open a plate matrix and select up to 12
plate, the matrix is displayed in quadrants; tap the 1-2-3-4 box to change the view.
Activity Before Read
• Set a delay (up to 2 hours, 45 minutes) before the read.
• Select a shake mode of
Orbital
to shake the plate before the read begins. Adjust the Frequency, if needed.
The cycles per minute updates as the slider moves. The measurement in
mm
indicates the distance the carrier travels during the shake.
When finished, tap Save. The protocol name is added to the
If your
Endpoint
reading the plate. From the
tab.
Agilent BioTek Epoch 2 Instructions for Use
1771011I Revision C
Take3
(if configured).
and (optional)
Secondary
Take3
only:
Normal
(optimum performance) or
options:
Linear
(in a figure-eight pattern). Set a
assay requires incubation, activate
Main
Menu, select
WL: Define the primary and (optional)
Sweep
Blank
(side-to-side),
Orbital
Duration
(up to 2 hours 45 minutes)
Temperature Control
Instrument
and tap the
Page 30 of 51
Cuvette
(optimum speed).
wells. For a 384-well
(circle), or
Double
Main
Menu.
before
Temperature
Agilent Technologies, Inc.
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