Create And Save A Protocol - Agilent Technologies BioTek Epoch 2 Instructions For Use Manual

Getting Started
When the temperature displayed at the top of the screen reaches the set point,
4.
move the carrier out, insert the plate, and move the carrier back in.
The minimum set point must be ambient room temperature plus 4°C.
Condensation Control
A temperature
using microplate lids. When no gradient (0) is applied, the heaters above and below
the plate operate at the same (setpoint) temperature. This is recommended if the
plate has no lid, or condensation is not a problem when lids are used.
When a gradient (0.1 to 2.0) is applied, the heaters above the plate operate slightly
higher than the set point and the heaters below the plate operate slightly lower
than the set point. The gradient represents the temperature separation between
the top and bottom heaters. This differential should serve to reduce or eliminate
condensation.
In the example below, the Set point is 37°C and the Gradient is 1.0:
Some experimentation with the gradient setting may be necessary to achieve
optimal results with your assay.

Create and Save a Protocol

From the Main Menu, tap
1.
Use the onscreen keyboard to enter a name for the protocol, then tap Save. Note
2.
that the protocol name is limited to 18 characters.
Set the Read Mode
3.
Define the protocol parameters and then tap Save.
4.
Agilent BioTek Epoch 2 Instructions for Use
1771011I Revision C
Gradient can be applied to reduce the risk of condensation when
37.4˚C
36.4˚C
Protocol
to Endpoint, Kinetic, or Spectral.
37.4˚C
36.4˚C
> Create.
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