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Getting Started
Kinetic Protocols
Time: Specify the full duration of the kinetic analysis, up to 272:15:00 (hh:mm:ss).
Note: You can stop a protocol while it is running, before the
will be saved.
Interval: Specify the interval (delay) between reads, up to 2:45:00 (hh:mm:ss). The
Interval must be shorter than the Time.
Number of kinetic
settings. Cannot exceed 100 reads.
Considerations: The Interval must be long enough to support the protocol parameters
and the number of microplate wells selected for reading. If a shake is specified, its
Duration may result in a longer interval than defined in the protocol. To test the
settings, save the protocol and return to the
select the wells if not reading the full plate, and tap Start. The Minimum Kinetic Interval
will display if the defined Interval is too short. Run the protocol using the displayed
interval, or return to the protocol and modify its parameters.
Plate: Options include standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates,
Cuvette (if equipped), and
Primary
WL: Define a wavelength in the range 200-999 nm. Only one wavelength is
supported through the touchscreen; use
requirements.
Microplates and
Read
Speed: Select
See the Specifications section to compare performance and timing specifications.
Blanks: Tap to open a plate matrix and select up to 12
plate, the matrix is displayed in quadrants; tap the 1-2-3-4 box to change the view.
Options (tap Edit):
If applicable, check
in the chamber after the last kinetic read.
If the assay requires incubation:
Agilent BioTek Epoch 2 Instructions for Use
1771011I Revision C
reads: Calculated automatically, based on the Time and Interval
Take3
(if configured).
Take3
only:
Normal
(optimum performance) or
Eject the plate when
Time
Main
Menu. Tap the protocol to run it,
Gen5
if your assay has other
Sweep
Blank
wells. For a 384-well
done. If unchecked, the plate will remain
Page 31 of 51
expires; results
(optimum speed).
Agilent Techologies, Inc.
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