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To apply the IRM solutions
You need to adjust the amount of solution to apply or its concentration to suit
your needs.
These compounds are designed to persist in the ionization region and are
difficult to remove completely. Therefore, for the first few applications, apply
smaller amounts until the response of your system under your analysis
conditions is known. Typically, 200 µL of the IRM working solution is applied
and allowed to evaporate; then 200 µL of FC-70 is applied. The wick holds
approximately 250 µL to 300 µL of solution before saturation occurs.
Before you begin, make sure that you have:
1 In the MassHunter Acquisition program, set the instrument to Standby
2 Briefly mix the
3 Load a pipette with 200 µL of
4 Carefully dispense the
Nanospray Ion Source User Guide
•
reagent-grade acetonitrile
•
IRM working solution
•
FC-70 liquid (p/n G1982-85002)
mode.
IRM working solution
homogeneity.
needed.
filling ports on the top of the Nanospray Ion Source.
on a vortex mixer to assure
IRM working
IRM working solution
Mass Reference Solution
solution. Adjust this volume as
evenly between the two IRM
2
47
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