Agilent Technologies G1364B User Manual
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Agilent Technologies G1364B User Manual

1100 series analytical and preparative scale fraction collectors
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Agilent 1100 Series
Analytical and
Preparative Scale
Fraction Collectors
User's Guide
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  • Page 1 Agilent 1100 Series Analytical and Preparative Scale Fraction Collectors User’s Guide...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2001, 2003 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced in The material contained in this docu- 1987). U.S. Government users will receive any form or by any means (including elec- ment is provided “as is,”...
  • Page 3 In This Guide… This manual contains technical reference information about the Agilent 1100 Series fraction collectors analytical and preparative scale. The manual describes the following: Configuration and Operation of the Fraction Collector This chapter describes the configuration and operation of the fraction collector including guidelines to optimize the system and to avoid problems.
  • Page 4 4Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 5: Table Of Contents

    Contents Configuration and Operation of the Fraction Collector Configuration of the Fraction Collector Configuration of the Fraction Collector in the ChemStation Configuring multiple fraction collectors Delay volumes and delay calibration Handling of Delay times and volumes Detector signal delay Performing a delay calibration with an UV detector Performing a delay calibration with an MSD Making Your Own Calibration Method Setting up a Fraction Collector Method...
  • Page 6 Contents Application Notes Troubleshooting and Test Functions Status Indicators Error Messages Maintenance Functions Transport Unit Self Alignment Status Indicators Power Supply Indicator Instrument Status Indicator Maintenance Functions Transport Unit Self Alignment Step Commands Troubleshooting Repairing the Fraction Collector Introduction into Repairing the Fraction Collector Simple Repairs Cleaning the Fraction Collector Overview of Main Repair Procedures...
  • Page 7 Contents List of Recommended Test Tubes List of Recommended Vials and Caps List of Recommended Plates and Closing Mats Transport Unit Assembly (Preparative Scale) Transport Unit Assembly (Analytical Scale) Needle Assemblies Diverter-Valve Assembly Tubing Kits Internal Tray Assembly Fraction Collector Accessory Kit Specifications Performance Specifications for the Fraction Collectors Safety Information...
  • Page 8 Contents Agilent Technologies on Internet Index Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 9: Configuration And Operation Of The Fraction Collector

    Configuration of the Fraction Collector Delay volumes and delay calibration Setting up a Fraction Collector Method Setting up a Fraction Collection Sequence Viewing your Results Special Applications Optimizing fraction collection Limitations and how to avoid problems Application Notes Agilent Technologies...

  • Page 10: Configuration Of The Fraction Collector

    Configuration of the Fraction Collector Configuration of the Fraction Collector Configuration of the Fraction Collector in the ChemStation Before using the preparative scale fraction collector G1364B the delay calibration CAU TI O N adapter G1364-87301 has to be removed from the needle carrier assembly.
  • Page 11 Configuration and Operation of the Fraction Collector Configuration of the Fraction Collector Figure 1 Fraction Collector Configuration dialog Use for Sample Recovery This checkbox only appears, if multiple fraction collectors are configured (see Figure 18 on page 39). Then the last fraction collector can be selected for sample recovery.
  • Page 12 Configuration and Operation of the Fraction Collector Configuration of the Fraction Collector Fraction Delay Volumes The Fraction Delay Volume specifies the volume between the detector cell and fraction collector needle tip. This volume has to determined by a delay calibration. See “Delay volumes and delay calibration”...
  • Page 13 Configuration and Operation of the Fraction Collector Configuration of the Fraction Collector vial/well bottom. During the continuing filling process the needle tip moves upwards while staying in contact with the surface of the collected liquid. This option is recommend for low flow rates and small fraction volumes to avoid air bubbles and accomplish an accurate fraction volume.

  • Page 14: Configuring Multiple Fraction Collectors

    Configuration and Operation of the Fraction Collector Configuration of the Fraction Collector Table 1 Syntax for the definition of Reserved Locations Location Syntax Description 1-4,12 Columns 1, 2, 3, 4 and 12 can’t be used Single locations G12,H12 Locations G12 and H12 can’t be used Configuring multiple fraction collectors To increase the capacity of the systems up to four fraction can be configured by using the Agilent 1100 Series selection valve G1160A.

  • Page 15: Delay Volumes And Delay Calibration

    Configuration and Operation of the Fraction Collector Delay volumes and delay calibration Delay volumes and delay calibration Handling of Delay times and volumes Figure 2 shows a schematic drawing of the fraction-collection part of the Agilent 1100 series purification system with the two delay volumes V .

  • Page 16: Detector Signal Delay

    Configuration and Operation of the Fraction Collector Delay volumes and delay calibration Figure 3 Chromatogram with peak start t0 and peak end tE Detector signal delay Every Agilent UV detector that is used for triggering fractions has an internal signal delay caused by filtering the raw data. The signal delay depends on the Peakwidth setting of the detector and is accounted for when the diverter valve is triggered.
  • Page 17 Configuration and Operation of the Fraction Collector Delay volumes and delay calibration If the internal signal delay is longer than the delay time t some of the peak will be lost. The maximum allowed signal delay time can be calculated using the following equation: ·...

  • Page 18: Performing A Delay Calibration With An Uv Detector

    3 Connect a bottle of water to Channel A. 4 Install the Delay Calibration Adapter G1364-87301 by attaching it to the needle carrier assembly (G1364B preparative scale only). 5 Switch to Diagnosis View (if necessary). 6 Open the AFC Delay Volume Calibration status window (see...
  • Page 19 Configuration and Operation of the Fraction Collector Delay volumes and delay calibration 7 Click the Continue button. The Info field gives the instruction to turn on the pumps (see Figure Figure 5 Delay Volume Calibration Status Window 8 When you have turned on the pumps, click the Continue button again to start the delay volume calibration.
  • Page 20 Configuration and Operation of the Fraction Collector Delay volumes and delay calibration 9 When the calibration run has finished, click OK. The Calibration Results panel (see Figure 6) is displayed. Figure 6 Delay Calibration Results Panel The Calibration Results panel contains a results table and the acquired chromatograms from the detector(s) and the fraction collector delay sensor.
  • Page 21 You still have the possibility to manually change the volumes for each fraction collector separately in the configure screen of the ChemStation for each module. Before using the preparative scale fraction collector G1364B the delay calibration CAU TI O N adapter G1364-87301 has to be removed from the needle carrier assembly.

  • Page 22: Performing A Delay Calibration With An Msd

    3 Connect a bottle of water to Channel A of the main pump. 4 Install the Delay Calibration Adapter G1364-87301 by attaching it to the needle carrier assembly (G1364B preparative scale only). 5 Connect a bottle of water with 0.1% acetic acid or ammonium formate to the make-up pump.
  • Page 23 Configuration and Operation of the Fraction Collector Delay volumes and delay calibration The delay time should be at least 5 seconds but may, in fact, be negative (see Figure Figure 7 Delay Volume Calibration results 16 If the measured delay time is greater than 5 seconds, you can skip to step 21, otherwise, continue with step...
  • Page 24 Configuration and Operation of the Fraction Collector Delay volumes and delay calibration 21 Perform the calibration again. Verify the time is sufficient (>5 seconds, see Figure Figure 8 Delay time greater than 5 seconds 22 Press the Calibrate button if you want to store the new calculated delay volume for the MSD in the Fraction Collector Configuration (see also “Configuration of the Fraction Collector in the ChemStation”...
  • Page 25 This way, there will be sufficient delay for the lower flow rates and new lengths will not need to be cut. Before using the preparative scale fraction collector G1364B the delay calibration CAU TI O N adapter G1364-87301 has to be removed from the needle carrier assembly.

  • Page 26: Making Your Own Calibration Method

    Configuration and Operation of the Fraction Collector Delay volumes and delay calibration Making Your Own Calibration Method You make your own calibration method by editing one of the default calibration methods: 1 Load the default calibration method: DGCALAS.M in the case of an analytical system and DGCALPS.M in the case of a preparative system.

  • Page 27: Setting Up A Fraction Collector Method

    Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Setting up a Fraction Collector Method In order to setup or change the method parameters of your fraction collector select Setup Fraction Collector from the Instrument menu or right-click on the fraction collector icon in the graphical user interface.
  • Page 28 Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Peak-based If Peak-based is selected the collection of fraction is triggered by the signal of the detector, e.g. diode array detector or mass selective detector. The detailed trigger conditions are specified in the Peak Detectors table. The Peak-based trigger mode overrules all settings in the Timetable below.
  • Page 29 Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Working Mode For each peak detector Threshold only, Threshold/Slope and Slope only are possible. In the Threshold only mode the settings for Up Slope, Down Slope and Upper Threshold in the subsequent columns are ignored. Fraction collection is triggered whenever the detector signal exceeds the specified threshold value.
  • Page 30 Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Whenever the Peak Based mode is specified fractions will be collected based on the peak detection parameters given in the Peak Detector table. Additionally a Maximum Peak Duration in minutes has to be specified. This parameter is mandatory if you use Peak Controlled fraction collection, but is disabled for Time Based fraction collection.
  • Page 31 Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Auxiliary In the Auxiliary section the Maximum fill volume per location can be specified. If as configured is selected, the pre-configured volume (see Instrument > Pre-configured Wellplate Types) is used. This ensures that the location (well, vial or tube) cannot be overfilled during fraction collection.

  • Page 32: Fraction Preview

    Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Fraction Preview To determine the appropriate fraction collection parameters the Agilent ChemStation provides a valuable tool that becomes accessible by pushing the button labelled Fraction Preview Tool (Figure 12) in the Peak Detectors section.
  • Page 33 Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method chromatogram can be zoomed, the values for up and down slope can be specified and the upper and lower threshold level can be set-up. The graphically specified values are automatically transferred to the Peak Detector Table.

  • Page 34: Setting Up A Fraction Collection Sequence

    Configuration and Operation of the Fraction Collector Setting up a Fraction Collection Sequence Setting up a Fraction Collection Sequence Assignment of Start Location for fraction collection The start location for fraction collection can either be assigned in the Sample Info 13) dialog box in the RunControl menu, in the Sequence Parameters (Figure 14) dialog box or in the Sequence Table in the Sequence menu.
  • Page 35 Configuration and Operation of the Fraction Collector Setting up a Fraction Collection Sequence Figure 14 Start Location in Sequence Parameters dialog box Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 36: Viewing Your Results

    Configuration and Operation of the Fraction Collector Viewing your Results Viewing your Results Data Analysis In order to display the tick marks for the collected fractions on the screen, click on Signal options from the Graphics menu. Then choose Separated in the Layout dropdown list.
  • Page 37 Configuration and Operation of the Fraction Collector Viewing your Results Figure 16 Fraction collection report setup Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 38: Special Applications

    Configuration and Operation of the Fraction Collector Special Applications Special Applications Pooling We define Pooling as collecting fractions from multiple injections into the same fraction locations. In the Agilent Chemstation, there are two ways to initiate pooling: • If you want to collect multiple injections from the one sample vial or sample well into the same fraction location, specify a Fract.

  • Page 39: Sample Recovery

    Configuration and Operation of the Fraction Collector Special Applications Sample Recovery The Agilent 1100 Series Fraction Collectors offers different possibilities for sample recovery: • The preferred recovery strategy is to install multiple fraction collectors in your LC systems and use the last of those fraction collectors for recovery. This recovery fraction collector can be selected in the Configuration dialog box as displayed in Figure...

  • Page 40: Semi-Preparative Operation

    Configuration and Operation of the Fraction Collector Special Applications With each start of a sequence run the recovery will start at the same positions. In order CAU TI O N to avoid contamination the vessels that contain the recovery fractions have to be exchanged.

  • Page 41: Using High Test Tubes In The Analytical Scale Fraction Collector

    Configuration and Operation of the Fraction Collector Special Applications Figure 19 Fraction collector configuration parameters for semi-prep operation Using high test tubes in the analytical scale fraction collector In the standard configuration the analytical scale fraction collector G1364C the maximum test tube height is limited to 48 mm. This limitation can be extended to 75 mm if •...

  • Page 42: Optimizing Fraction Collection

    Configuration and Operation of the Fraction Collector Optimizing fraction collection Optimizing fraction collection Time-based • Time slices must have a length of at least 0.05 min. fraction • Set # of Fractions such that length of resulting fractions is at least 0.05 min. collection Peak-based •...

  • Page 43: Limitations And How To Avoid Problems

    Configuration and Operation of the Fraction Collector Limitations and how to avoid problems Limitations and how to avoid problems Rinse Fraction If Rinse Fraction Collection Needle is set to Between fraction collection, at Collection Needle least 0.3 min are required to perform this task. When doing time-based fraction collection rinsing the needle is only possible between two time table entries, which must have a gap of at least 0.3 min.

  • Page 44: Application Notes

    Systems are available from a series of application notes. Printed versions can ordered form Agilent or pdf-files can be downloaded from the Agilent Website http://www.chem.agilent.com Table 4 Selected Agilent Technologies Application Notes Title Publication Number An optimized system for analytical and preparative work...

  • Page 45: Troubleshooting And Test Functions

    Agilent 1100 Series Fraction Collectors User’s Guide Troubleshooting and Test Functions Status Indicators Maintenance Functions Maintenance Functions Transport Unit Self Alignment Step Commands Agilent Technologies...

  • Page 46: Status Indicators

    Troubleshooting and Test Functions Status Indicators The fraction collector is provided with two status indicators which indicate the operational state (prerun, not ready, run, and error states) of the instrument. The status indicators provide a quick visual check of the operation of the fraction collector (see “Status Indicators”...

  • Page 47: Status Indicators

    Troubleshooting and Test Functions Status Indicators Status Indicators Two status indicators are located on the front of the fraction collector. The lower left indicates the power supply status, the upper right indicates the fraction collector status. Status indicator green/yellow/ Line power switch with green light Figure 20...

  • Page 48: Instrument Status Indicator

    Troubleshooting and Test Functions Status Indicators Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions: • When the status indicator is OFF (and power switch light is on), the instrument is in a prerun condition, and is ready to begin an analysis. •...

  • Page 49: Maintenance Functions

    Troubleshooting and Test Functions Maintenance Functions Maintenance Functions Some maintenance procedures require the needle arm, and needle carrier to be moved to specific positions to enable easy access to components. The maintenance functions move these assemblies into the appropriate maintenance position. In the ChemStation the fraction collector maintenance in the Diagnosis view (see positions can be selected from the Maintenance menu...
  • Page 50 Troubleshooting and Test Functions Maintenance Functions • The needle carrier assembly • The valve to needle tubing. • The diverter valve. After the maintenance or repair task has been finished, click End to move the transport assembly to the position Home Home Position This maintenance function moves the arm up and to the right rear for better...

  • Page 51: Transport Unit Self Alignment

    Troubleshooting and Test Functions Transport Unit Self Alignment Transport Unit Self Alignment The transport unit alignment with the transport unit and the well-plate tray is required to compensate for larger deviations in positioning the needle carrier assembly. The transport unit self alignment is required after disassembling the system or when you exchange the transport unit, the needle carrier assembly or the MTP main board.
  • Page 52 Troubleshooting and Test Functions Transport Unit Self Alignment How to perform a Transport Unit Self Alignment? Steps Comments 3 Ensure that the wellplate adapter is correctly assembled 4 Select the Maintenance menu in the Diagnosis view of the Agilent ChemStation. 5 In the menu choose Fraction Collector >...

  • Page 53: Step Commands

    Troubleshooting and Test Functions Step Commands Step Commands Some movements of the fraction collection sequence can be done under manual control. This is useful during troubleshooting where close observation of each of the fraction collection step is required to confirm a specific failure mode or verify successful completion of a repair.

  • Page 54: Troubleshooting

    Troubleshooting and Test Functions Step Commands Table 5 Step Commands Step Action Comments Needle Up Lifts the needle arm to the upper Command also switches the position. diverter valve to waste if it is not already in that position. Needle into vessel Lowers the needle into the specified only analytical scale G1364C vessel.

  • Page 55: Repairing The Fraction Collector

    Agilent 1100 Series Fraction Collectors User’s Guide Repairing the Fraction Collector Cleaning the Fraction Collector Overview of Main Repair Procedures Simple Repairs Agilent Technologies...

  • Page 56: Introduction Into Repairing The Fraction Collector

    Repairing the Fraction Collector Introduction into Repairing the Fraction Collector Introduction into Repairing the Fraction Collector Simple Repairs The fraction collector is designed for easy repair. The most frequent repairs such as changing a needle assembly, the capillary (micro scale) or tubings can be done from the front of the instrument with the instrument in place in the system stack.

  • Page 57: Overview Of Main Repair Procedures

    Plug channel with air leak sensor channel adapter underneath Tray compartment divider Figure 23 Main Assemblies (Example displays the analytical scale module G1364C. The preparative scale fraction collector G1364B contains a different needle) Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 58: Simple Repairs

    Repairing the Fraction Collector Simple Repairs Simple Repairs The procedures described in this section can be done with the fraction collector in place in the stack. These procedures can be done on a more frequent basis. Table 6 Simple Repair Procedures Procedure Typical Frequency Notes...

  • Page 59: Replacing The Inlet/Waste Tubings

    Repairing the Fraction Collector Simple Repairs Replacing the Inlet/Waste Tubings Frequency When contaminated, worn out or visibly damaged Typically once every year Tools required None Parts Required Inlet / waste tubing assembly included in tubing kit preparative scale 0.8 mm ID, PN G1364-68711 or Inlet / waste tubing assembly included in tubing kit analytical scale 0.25 mm ID, PN G1364-68712 To avoid personal injury, keep fingers away from the needle area during fraction...
  • Page 60 Repairing the Fraction Collector Simple Repairs Before beginning this procedure: 1 Locate the diverter valve with the finger-tight fittings of the inlet / waste tubing • Position the transport unit of the fraction assembly (the figure shows the open fraction collector in the “Home Position”...
  • Page 61 Repairing the Fraction Collector Simple Repairs 4 Install the new inlet / waste tubing assembly 5 The rear tubing (with the label with the into the bolt carrier as shown below. Slide in arrow) must be connected to the flow cell of the long ends of the tubings from bottom to the detector.
  • Page 62 Repairing the Fraction Collector Simple Repairs 8 IMPORTANT: The cables must run into the On completion of this procedure: ports of the diverter valve in lines parallel • Re-install the tray(s) in the tray base. to the horizon. • Start the instrument. •...

  • Page 63: Replacing The Valve To Needle Tubing

    Repairing the Fraction Collector Simple Repairs Replacing the Valve to Needle Tubing Frequency When contaminated, worn out or visibly damaged Typically once every year Tools required Wrench, open end, 4mm, PN 8710-1534 (supplied in accessory kit) Wrench, open end, 1/4 – 5/16 inch, PN 8710-0510 (supplied in accessory kit) Parts Required Valve to needle tubing assembly included in tubing kit preparative scale 0.8 mm ID, PN G1364-68711 or valve to needle tubing assembly included in...
  • Page 64 Repairing the Fraction Collector Simple Repairs 2 Unscrew the finger-tight fitting of the valve 3 Using the 4 mm wrench and the 5/16“ to needle tubing assembly at the diverter wrench for counter-holding unscrew the valve. valve to needle tubing from the needle. (Viewed from the bottom) 4 Un-clip the tubing from the bottom of the 5 Slide the tubing through the hole in the...
  • Page 65 Repairing the Fraction Collector Simple Repairs 6 Install the new valve to needle tubing 7 Using the 4 mm wrench and the assembly by clipping it in to the holder in the 5/16“ wrench for counter-holding connect z-arm assembly (1), Important!!!) and slide the valve to needle tubing to the needle.

  • Page 66: Exchanging The Preparative Needle Assembly

    Repairing the Fraction Collector Simple Repairs Exchanging the Preparative Needle Assembly Frequency When the needle is leaky or visibly damaged When the needle is blocked or contaminated Tools required Wrench, open end, 4mm, PN 8710-1534 (supplied in accessory kit) Wrench, open end, 1/4 – 5/16 inch, PN 8710-0510 (supplied in accessory kit) Parts required Preparative needle assembly, G1364-87201 To avoid personal injury, keep fingers away from the needle area during fraction...
  • Page 67 Repairing the Fraction Collector Simple Repairs 2 Holding the needle assembly between your 3 Insert the new needle assembly into the thumb and forefinger, slide out the assembly holder of the needle carrier assembly. Make towards the rear of the needle carrier sure to push it all the way to the front.

  • Page 68: Exchanging The Analytical Needle Assembly

    Repairing the Fraction Collector Simple Repairs Exchanging the Analytical Needle Assembly Frequency When the needle is visibly damaged When the needle is blocked or contaminated Tools required Wrench, open end, 4mm, PN 8710-1534 (supplied in accessory kit) Wrench, open end, 1/4 – 5/16 inch, PN 8710-0510 (supplied in accessory kit) Parts required Analytical needle assembly, G1364-87203 To avoid personal injury, keep fingers away from the needle area during fraction...
  • Page 69 Repairing the Fraction Collector Simple Repairs 2 Remove the wellplate adapter by turning it 3 Insert the new needle assembly into the clockwise (1). Hold the needle assembly holder of the needle carrier assembly. Make between your thumb and forefinger, slide out sure to push it all the way to the front.
  • Page 70 Repairing the Fraction Collector Simple Repairs Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 71: Exchanging The Diverter Valve

    Repairing the Fraction Collector Simple Repairs Exchanging the Diverter Valve Frequency When leaky or defective Tools required Hex key 2.0 mm, PN 8710-2438 (supplied in accessory kit) Parts required Diverter Valve, PN G1364-61901 Before beginning this procedure: 1 Locate the diverter valve with the finger tight fittings of the inlet / waste tubing assembly •...
  • Page 72 Repairing the Fraction Collector Simple Repairs 4 Disconnect the power cable of the diverter 5 Using the 2 mm hex key loosen the holding valve from the connector on the front of the screw of the diverter valve and remove the z-arm.
  • Page 73 Repairing the Fraction Collector Simple Repairs 8 Connect the finger tight fittings of the inlet / 9 A color coded ring on one of the tubings and waste tubing assembly to the ports of the the valve body indicates, which cable belongs diverter valve.

  • Page 74: Exchanging The Internal Tray

    Repairing the Fraction Collector Simple Repairs Exchanging the Internal Tray Frequency When defective Tools required None Parts required Internal tray analytical scale, (G1364-63113) Internal tray preparative scale,(G1364-63114) Before beginning this procedure: 1 Locate the internal tray assembly with the rinse funnel and flow delay sensor in the •...
  • Page 75 Repairing the Fraction Collector Simple Repairs 2 Remove the internal tray by pushing down 3 Remove the corrugated waste tubing from the plastic holder that holds it in position the front of the instrument (1) and slide the underneath the metal latch (1) and sliding internal tray’s waste tubing to the rear of the the tray to the left at the same time (2).

  • Page 76: Repairing Or Exchanging A Funnel Of The Internal Tray

    Repairing the Fraction Collector Simple Repairs Repairing or Exchanging a Funnel of the Internal Tray Frequency When leaky or contaminated Tools required None Parts required Funnel assembly, waste tubing assembly, seals (for part numbers see “Internal Tray Assembly” on page 92) Before beginning this procedure: 1 Remove the internal tray from the instrument, see...
  • Page 77 Repairing the Fraction Collector Simple Repairs 4 Exploded view for the parts of the internal 5 Slide the waste tubing of the newly tray. Once the funnel has been removed from assembled funnel assembly through the slit the internal tray, it can be disassembled and beside the funnel in the internal tray defective parts can be replaced (funnel screw assembly...
  • Page 78 Repairing the Fraction Collector Simple Repairs 8 Insert the funnel into the tray and screw it 9 Re-install the plug that holds the flow delay tight (clockwise) sensor (1), pull the waste tubing tight through the flow delay sensor (2) and mount the waste tubing (3).

  • Page 79: Parts And Materials

    List of Recommended Test Tubes List of Recommended Vials and Caps List of Recommended Plates and Closing Mats Transport Unit Assembly (Preparative Scale) Transport Unit Assembly (Analytical Scale) Needle Assemblies Diverter-Valve Assembly Tubing Kits Internal Tray Assembly Fraction Collector Accessory Kit Agilent Technologies...

  • Page 80: Supported Trays For Fraction Collectors

    Parts and Materials Supported Trays for Fraction Collectors Supported Trays for Fraction Collectors Table 7 Trays for the Fraction Collector Item Description Part Number Full tray for 215 test tubes, 12x100 mm G1364-84506 Full tray for 126 test tubes, 16x100 mm G1364-84505 Full tray for 60 round bottom tubes, 25x100 mm G1364-84504...
  • Page 81 Parts and Materials Supported Trays for Fraction Collectors The items in this figure are displayed in different scaling factors! Figure 24 Trays Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 82: List Of Recommended Test Tubes

    Parts and Materials List of Recommended Test Tubes List of Recommended Test Tubes Table 8 Round Bottom Test Tubes Description Volume (ml) lOO/Pack 25 x 100 mm, clear glass 5042-6459 30 x 100 mm, clear glass 5042-6458 30 x 48 mm 5042-6470 16 x 48 mm 5022-6533...

  • Page 83: List Of Recommended Vials And Caps

    Parts and Materials List of Recommended Vials and Caps List of Recommended Vials and Caps Table 9 Crimp Top Vials (Caps for Use with the Analytical Scale Fraction Collector, only!) Description Volume (ml) lOO/Pack lOOO/Pack lOO/Pack (silanized) Clear glass 5181-3375 5183-4491 Clear glass, 5182-0543...
  • Page 84 Parts and Materials List of Recommended Vials and Caps Table 12 Crimp Caps (Caps for Use with the Analytical Scale Fraction Collector, only!) Description Septa 100/Pack Silver aluminum Clear PTFE/red rubber 5181-1210 Silver aluminum Clear PTFE/red rubber 5183-4498 (1000/Pack) Blue aluminum Clear PTFE/red rubber 5181-1215 Green aluminum...

  • Page 85: List Of Recommended Plates And Closing Mats

    Parts and Materials List of Recommended Plates and Closing Mats List of Recommended Plates and Closing Mats Table 15 Recommended Plates and Closing Mats (Std. Well Plates and Closing Mats for Use with the Analytical Scale Fraction Collector, only!) Item Description Volume (ml) Package...
  • Page 86 Parts and Materials List of Recommended Plates and Closing Mats Closing mats with adhesive can give some contamination in the system. The WA RN ING adhesive is soluble in most of the solvents used in HPLC. In general do not use closing mats with adhesive. The fraction collector has no WA RN ING prepunch needle, therefore the adhesive will clog the needle after several injections.

  • Page 87: Transport Unit Assembly (Preparative Scale)

    Parts and Materials Transport Unit Assembly (Preparative Scale) Transport Unit Assembly (Preparative Scale) Table 16 Transport Unit Assembly (Preparative Scale) Item Description Part Number Transport Unit Assembly (Preparative Scale), G1364-60018 includes items 2 and 4 2a, b Tubing - diverter valve to needle, 0.8 mm ID G1364-86711 Diverter valve assembly G1364-61901...

  • Page 88: Transport Unit Assembly (Analytical Scale)

    Parts and Materials Transport Unit Assembly (Analytical Scale) Transport Unit Assembly (Analytical Scale) Table 17 Transport Unit Assembly (Analytical Scale) Item Description Part Number Transport unit assembly (analytical scale), G1364-60019 includes items 2 and 4 2a, b Tubing - diverter valve to needle, 0.25 mm ID G1364-68712 Diverter valve assembly G1364-61901...

  • Page 89: Needle Assemblies

    Parts and Materials Needle Assemblies Needle Assemblies Table 18 Needle Assemblies Item Description Part Number Needle assembly preparative scale G1364-87201 Needle assembly analytical scale (20 mm needle) G1364-87202 For use in the analytical scale fraction collector at flow rates > 10 ml/min.

  • Page 90: Diverter-Valve Assembly

    Parts and Materials Diverter-Valve Assembly Diverter-Valve Assembly Table 19 Diverter-Valve Assembly (preparative and analytical scale) Item Description Part Number Diverter-valve assembly G1364-61901 PIN screw 0515-1211 Figure 28 Diverter-Valve Assembly Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 91: Tubing Kits

    Parts and Materials Tubing Kits Tubing Kits Table 20 Tubing Kits Item Description Part Number Tubing kit preparative scale 0.8 mm ID (consists of G1364-68711 items 2,and 3) Inlet/waste tubing assembly preparative scale 0.8 mm ID Order Item 1 Diverter valve to needle tubing preparative scale 0.8 mm ID Order Item 1 Tubing kit analytical scale 0.25 mm ID (consists of G1364-68712...

  • Page 92: Internal Tray Assembly

    Parts and Materials Internal Tray Assembly Internal Tray Assembly Table 21 Internal Tray Assembly (Analytical and Preparative Scale) Item Description Part Number Internal tray assembly analytical scale, includes items 2 – 7 G1364-63114 Internal tray assembly preparative scale, includes items 2 – 7 G1364-63113 Screw-seat-adapter 5022-2200...

  • Page 93: Fraction Collector Accessory Kit

    Parts and Materials Fraction Collector Accessory Kit Fraction Collector Accessory Kit Table 22 Fraction Collector Accessory Kit Contents G1364-68705 Description Quantity Part Number Wrench, open end, 4mm 8710-1534 Wrench, open end, 1/4-5/16 inch 8710-0510 Hex key 2.0 mm 8710-2476 Finger tight fittings, 1/16” f-120 0100-1516 †...
  • Page 94 Parts and Materials Fraction Collector Accessory Kit Agilent 1100 Series Fraction Collectors User’s Guide...

  • Page 95: Specifications

    Agilent 1100 Series Fraction Collectors User’s Guide Specifications Performance Specifications for the Fraction Collectors Agilent Technologies...

  • Page 96: Performance Specifications For The Fraction Collectors

    Specifications Performance Specifications for the Fraction Collectors Performance Specifications for the Fraction Collectors Table 23 performance Specifications Agilent 1100 Series PREPARATIVE SCALE Fraction Collector (G1364B) Type Specification trigger modes Time slices, Peak (threshold, up- / downslope), Timetable (combination of time intervals and peak) and...
  • Page 97 Safety features Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors * Vials can be used as recommended by Agilent Technologies (see “List of Recommended Vials and Caps” on page 83 and “List of Recommended Plates and Closing Mats”...
  • Page 98 Specifications Performance Specifications for the Fraction Collectors Table 24 Performance Specifications Agilent 1100 Series ANALYTICAL SCALE Fraction Collector (G1364C) Type Specification trigger modes Time slices, Peak (threshold, up- / downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323B Control Module) Agilent 1100 UV-Vis detectors (G1314A, G1315A/B, G1365 A/B and the Agilent G1946C/D, G1956A/B LC-MSD are fully supported.
  • Page 99 Specifications Performance Specifications for the Fraction Collectors Table 24 Performance Specifications Agilent 1100 Series ANALYTICAL SCALE Fraction Collector (G1364C) (continued) Type Specification maximum tube / plate 48 mm with long needle assembly G1367-87200 height 75 mm with short needle assembly G1364-87202 Maximum tube volume ca.
  • Page 100 Specifications Performance Specifications for the Fraction Collectors * Vials and well-plates and capped vials and well plates with closing mats can be used as recom- mended by Agilent Technologies (see “List of Recommended Vials and Caps” on page 83 and “List...
  • Page 101 Agilent 1100 Series Fraction Collectors User’s Guide Safety Information Safety Information Lithium Batteries Information Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent Technologies...

  • Page 102: A Safety Information

    Safety Information Safety Information Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.

  • Page 103: Operation

    Safety Information Safety Information Operation Before applying power, comply with the installation section. Additionally the following must be observed. Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto-transformers, and devices connected to it must be connected to a protective earth via a ground socket.

  • Page 104: Safety Symbols

    Safety Information Safety Information Safety Symbols Table 25 shows safety symbols used on the instrument and in the manuals. Table 25 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage.

  • Page 105: Lithium Batteries Information

    Safety Information Lithium Batteries Information Lithium Batteries Information Danger of explosion if battery is incorrectly replaced. Replace only with the same or WA RN ING equivalent type recommended by the equipment manufacturer. Lithium batteries may not be disposed-off into the domestic waste. Transportation of discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed.

  • Page 106: Radio Interference

    Safety Information Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...

  • Page 107: Sound Emission

    Safety Information Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...

  • Page 108: Solvent Information

    Safety Information Solvent Information Solvent Information Observe the following recommendations on the use of solvents. This instrument should only be used with solvents that have an ignition temperature WA RN ING higher than 200 Solvents Brown glass ware can avoid growth of algae. Always filter solvents, small particles can permanently block the capillaries.

  • Page 109: Agilent Technologies On Internet

    Safety Information Agilent Technologies on Internet • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com...
  • Page 110 Safety Information Agilent Technologies on Internet Agilent 1100 Series Fraction Collectors User’s Guide...
  • Page 111 Index delay volumes, 97, into location, detector peakwidth, above location, detectors, accessory kit, diverter valve, 97, liquid contact control, Agilent on internet, diverter valve assembly, analytical needle assembly, application notes, arm length calibration, maintenance functions, 46, error messages, 46, home position, park arm position, parts exchange position, battery...
  • Page 112 Index accessory kit, report, closing mats, reserved locations, UIB, diverter valve assembly, rinsing, 30, universal interface box, internal tray, upper threshold, needle assemblies, plates, safety features, recommended tubes, safety information recommended vials and caps, valve to needle tubings, on lithium batteries, sheet metal kit, vial contents temperature, sample recovery, 11,...
  • Page 114 • configuration and operation of the fraction collector, • troubleshooting and test functions, • simple repairs • parts and materials, • specifications, • safety information.  Agilent Technologies, Deutschland GmbH 2001, 2003 Printed in Germany 07/03 *G1364-90001* *G1364-90001* G1364-90001 Agilent Technologies...

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