Thermo Scientific KingFisher Apex User Manual
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Thermo Scientific KingFisher Apex User Manual

Thermo Scientific KingFisher Apex User Manual

Purification system
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KingFisher
™
USER GUIDE
and BindIx Software version 1.1
Catalog Numbers  5400910, 5400920, 5400930, 5400940
Publication Number   MAN0018970
Revision   D
For Laboratory Use.
Not for Clinical Use in China.
Apex Purification System

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  • Page 1 KingFisher Apex Purification System ™ USER GUIDE and BindIx Software version 1.1 Catalog Numbers  5400910, 5400920, 5400930, 5400940 Publication Number   MAN0018970 Revision   D For Laboratory Use. Not for Clinical Use in China.
  • Page 2 Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. Revision history:  MAN0018970 D (English) Revision Date Description 21 March 2025 The "Preferred use"...

  • Page 3: Table Of Contents

    Not for Clinical Use in China. Contents ■ CHAPTER 1   Product information ..........6 Product description .
  • Page 4 Not for Clinical Use in China. Contents Turn off instrument ............. 43 Change heat block/magnetic head .
  • Page 5 Not for Clinical Use in China. Contents ■ APPENDIX B   Maintenance ............88 Regular and preventive maintenance .

  • Page 6: Chapter 1   Product Information

    Not for Clinical Use in China. Product information Product description The Thermo Scientific KingFisher Apex Purification System is intended for automated transfer and ™ ™ processing of magnetic particles in a microplate format. The system utilizes magnetic rods covered with a disposable, specially designed tip comb, and plates.

  • Page 7: Ordering Configurations

    Not for Clinical Use in China. Chapter 1 Product information Ordering configurations Ordering configurations The contents of the KingFisher Apex Purification System are listed in the following table. ™ Component Cat. No. 5400910 Cat. No. 5400920 Cat. No. 5400930 Cat. No. 5400940 Instrument KingFisher Apex Purification System...

  • Page 8: Product Overview

    Not for Clinical Use in China. Chapter 1 Product information Product overview Product overview Instrument overview The KingFisher Apex Purification System consists of an instrument with touch screen control and a ™ process chamber with lid to protect samples against environmental contamination. Figure 1   KingFisher Apex instrument front view ™...

  • Page 9: Internal Layout

    Not for Clinical Use in China. Chapter 1 Product information Product overview Internal layout The process chamber of the KingFisher Apex instrument consists of a turntable with eight plate ™ stations. The loading position has an illuminated display, while the processing position is situated over the heating block (there are two heating block stations that can be selected for the processing position), and underneath the processing head.

  • Page 10: User Interface Overview

    Not for Clinical Use in China. Chapter 1 Product information Product overview User interface overview Symbol Function Open Notifications screen (See page 48 for more details) Swipe down from the top of the touch screen Displays the following information: • Notifications • Internet status •...
  • Page 11 Not for Clinical Use in China. Chapter 1 Product information Product overview Users and roles Note: User roles are only active when Login access is selected. There are four different user roles, each with different rights: • Administrator: The administrator has no limitations for instrument use. The administrator can create, modify, and delete other roles.

  • Page 12: Technology Overview

    Not for Clinical Use in China. Chapter 1 Product information Technology overview Technology overview Principle of magnetic particle processing The KingFisher Apex instrument operates on inverse magnetic particle processing (MPP) technology. ™ Instead of transferring reagents in and out of plates, as might occur with an external magnet method, the magnetic particles are moved through a series of plates containing specific reagents with the aid of magnetic rods covered with a disposable, specially designed plastic tip comb.
  • Page 13 Not for Clinical Use in China. Chapter 1 Product information Technology overview Collecting magnetic particles During the collection of the magnetic particles, the magnetic rod is fully inside the tip. The magnetic rods together with the tip comb move slowly up and down in the plate and the magnetic particles are collected onto the edge of the tips.

  • Page 14: Upon Receiving The Device

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Concentration Magnetic particle processing allows changes in volume to be performed during the procedure. Transferring the magnetic particles from a plate with a larger volume to a plate with a smaller volume concentrates the sample.

  • Page 15: Precautions For Use

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device • Any damage claims must be filed with the carrier. Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit, but the manufacturer will make every effort to help obtain restitution from the carrier.

  • Page 16: Guidelines For Unpacking The Instrument

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Guidelines for unpacking the instrument • Make sure to place the instrument on firm table and allow min 5 cm (2 in.) free space around instrument. • To prevent condensation, the instrument should be left in its protective, antistatic plastic wrapping until the ambient temperature has been reached.

  • Page 17: First Time Instrument Setup

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device First time instrument setup 1. Connect the power supply cable to the power inlet. The instrument operates at voltages of 100–240 VAC and the frequency range of 50/60 Hz. Ensure that the local supply voltage in the laboratory conforms to that specified on the type label on the back of the instrument 2.

  • Page 18: Turn On The Instrument

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Turn on the instrument 1. Turn on the power switch at the rear of the instrument. 2. Press the on/off button to turn on the instrument. Select preferred use mode The instrument can be used without creating credentials.
  • Page 19 Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device IMPORTANT! If is selected, a username and password must be created. If this selection is changed after the username and password is created, the instrument resets user information and run history but retains protocols.
  • Page 20 Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Note: Create a username and password that contains at least six characters, and can include upper- and lower-case letters, numbers, and special characters. Not all characters are supported (e.g.

  • Page 21: User Login

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device User login After the username and password are created, the Local-Login screen appears each time the user powers on the instrument. Figure 9   Local-Login screen Username Password Localization settings The administrator and the scientist user can modify these settings: •...

  • Page 22: Set Date And Time

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Set date and time 1. Select (Change) under Date and time 2. Scroll through the numerical menus to select the time and date. 3. Select Set time zone 1.

  • Page 23: Set Language

    Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Set language 1. Select the Language field to open the drop down menu. 2. Select the desired language (English, Spanish, French, German, Portuguese, Italian, Russian, Japanese, Chinese). 3. Select KingFisher Apex Purification System User Guide ™...
  • Page 24 Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Install heating block 1. Select Menu4Instrument. See “Menu screen” page 49. 2. Select Insert for the position (1 or 2) to install the heating block. 3. Scan the heating block to be installed with the barcode reader or perform manual selection with the menu.
  • Page 25 Not for Clinical Use in China. Chapter 1 Product information Upon receiving the device Install magnetic head 1. Select Menu4Instrument. 2. Select Insert for the position (1 or 2) to install the magnet. 3. Scan the magnetic head to be installed with the barcode reader or perform manual selection with the menu.

  • Page 26: System Components

    Not for Clinical Use in China. Chapter 1 Product information System components System components Heating block and corresponding plate The instrument uses specially designed barcoded plates for optimal processing. Use of other types of plates may damage the instrument and compromise the warranty, as well as result in below optimal performance.
  • Page 27 Not for Clinical Use in China. Chapter 1 Product information System components (continued) Heating block Plate KingFisher Apex PCR Heating Block, PCR plate (10–80 µL ), Skirted PCR Armadillo (Cat. No. ™ ™ barcoded (Cat. No. 24075910) AB2396), Semi-Skirted PCR Armadillo (Cat. No. AB2496), ™...

  • Page 28: Magnetic Head

    Not for Clinical Use in China. Chapter 1 Product information System components (continued) Heating block Plate Adapter KingFisher Apex 24 Storage Tube KingFisher Apex 96 PCR Semiskirted Plate Adapter (Cat. No. ™ ™ Adapter (Cat. No. N21445) for use with N21446) for use with Semi-skirted PCR Armadillo (Cat.

  • Page 29: Tip Comb And Plate Compatibility

    Not for Clinical Use in China. Chapter 1 Product information System components (continued) Magnetic head Tip comb KingFisher Apex 96 Combi Head, barcoded KingFisher Apex 96 Combi Tip Comb, barcoded (Cat. ™ ™ (Cat. No. 24079920) No. 97002570) (also KingFisher 96 Tip Comb for Deep-Well Magnets) ™...

  • Page 30: Chapter 2   Methods

    Not for Clinical Use in China. Methods Protocol access overview There are several ways to access KingFisher protocols. ™ • Download protocols from theKingFisher Apex Protocol Library directly from the instrument ™ (requires an internet connection, but does not require setting up a Connect account). Note: Protocols in the Protocol Library are optimized for kits from Thermo Fisher Scientific.
  • Page 31 Not for Clinical Use in China. Chapter 2 Methods Protocol access overview Create a Connect Platform account 1. Go to thermofisher.com/connect from your web browser. 2. Click Sign up now and follow the prompts to create an account. Your e-mail address is used as your username. 3.
  • Page 32 Not for Clinical Use in China. Chapter 2 Methods Protocol access overview Add an instrument to your Connect Platform account The Connect Platform supports access to the KingFisher Apex instrument with the InstrumentConnect ™ application on your mobile device or from a web browser. When the instrument is connected, real-time instrument status can be viewed from the InstrumentConnect application.

  • Page 33: Download Protocols From Connect

    Not for Clinical Use in China. Chapter 2 Methods Protocol access overview Access your Connect account from an instrument 1. Swipe down to open the Notifications screen. 2. Select Sign in. Note: If another user account is displayed, select the username to sign out and connect a different user account.

  • Page 34: Change Password

    Not for Clinical Use in China. Chapter 2 Methods Change password Change password 1. Select User Settings from Menu. 2. Click the Change the password tab. 3. Enter your current password, then enter the new password twice. 4. Click Confirm or exit without changing the password. Create, edit, or delete users To create a new user, you must be logged in as Administrator.

  • Page 35: Run Purification Kit

    Not for Clinical Use in China. Chapter 2 Methods Run purification kit Run purification kit 1. Prepare reagents and samples and place them in the appropriate plates according to the instructions for your kit of choice. 2. Select a protocol and press on the Home screen.
  • Page 36 Not for Clinical Use in China. Chapter 2 Methods Run purification kit 5. (Optional): • Select to hide (if protocol run has been started) or cancel (if protocol run has not yet been started) protocol run, then return to the selection screen. •...

  • Page 37: Pause Or Abort Run

    Not for Clinical Use in China. Chapter 2 Methods Pause or abort run 12. (Optional) Review the run report (see “Run history overview” page 78). 13. (Optional) Export the report to a USB memory device (see “Export protocol to a USB memory device” page 78).

  • Page 38: Import Well Notes

    Not for Clinical Use in China. Chapter 2 Methods Import well notes Import well notes 1. Select a protocol to add well notes to then press on the Home screen. Note: A new window will open. 2. Click Add Lot information. Add lot information KingFisher Apex Purification System User Guide ™...
  • Page 39 Not for Clinical Use in China. Chapter 2 Methods Import well notes 3. Click Well notes to add notes or Reagents to add reagent lot information. To import notes from csv file click Import. Reagents Well notes Import KingFisher Apex Purification System User Guide ™...
  • Page 40 Not for Clinical Use in China. Chapter 2 Methods Import well notes 4. Locate .csv file and select .csv file then select KingFisher Apex Purification System User Guide ™...

  • Page 41: Edit Well Notes

    Not for Clinical Use in China. Chapter 2 Methods Edit well notes Edit well notes Once well notes are imported, a filled well note is presented with the # symbol. This can be edited. 1. Well notes can be edited by, selecting the well note in the grid then editing the note. Well notes Import 2.

  • Page 42: Export Well Notes

    Not for Clinical Use in China. Chapter 2 Methods Export well notes Export well notes 1. Navigate to Run history screen from Home screen. Home screen Run history scrren 2. Select Run that had well notes then export. Note: This will export report in pdf, XML format, and well notes only in csv format. Create .csv file The .csv file format needs to be in the format of "Coordinates, WellNote"...

  • Page 43: Turn Off Instrument

    Not for Clinical Use in China. Chapter 2 Methods Turn off instrument Turn off instrument 1. If logged in, on the Home screen select User Settings, then click Logout to log off from the instrument. 2. Return to the Local-Login screen. 3. Press the power switch and wait for the instrument to shut down. 4.

  • Page 44: Change Heat Block/Magnetic Head

    Not for Clinical Use in China. Chapter 2 Methods Change heat block/magnetic head Change heat block/magnetic head Heat blocks and magnetic heads can be changed depending upon the needs of the protocol. Access the Instrument screen (see “Instrument screen” page 58) to unload an existing heat block/magnetic head, and install a different heat block/magnetic head in their place.

  • Page 45: Unload Magnetic Head

    Not for Clinical Use in China. Chapter 2 Methods Change heat block/magnetic head Unload magnetic head 1. Select Menu4Instrument. 2. Select Unload for the position (1 or 2) you want to remove the magnet from. 3. Turn the handle to release the magnet. 4.

  • Page 46: Chapter 3   Software Applications

    Not for Clinical Use in China. Software applications About the user interface The user interface for the KingFisher Apex instrument and BindIx Software allows the user to perform ™ ™ the following actions: • Create a plate layout • Create, modify, run, save, and delete existing protocols •...
  • Page 47 Not for Clinical Use in China. Chapter 3 Software applications About the user interface Minimum PC requirements for BindIx Software ™ BindIx Software is a Windows UWP app. It can be obtained and installed through the Microsoft Store ™ ™ or installed offline from an app installer file (*.msixbundle) by “side-loading”. If installed via side-loading, the PC’s Microsoft Store services are used for installation.

  • Page 48: Overview Of User Interface Controls

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Overview of user interface controls Home screen The Home screen on the KingFisher Apex instrument is used to select or run protocols. Several ™ options are available for accessing protocols. For a description of the Home screen for the BindIx Software, see the “Menu screen”...

  • Page 49: Menu Screen

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Menu screen The Menu screen is the primary means of navigation between screens for the different applications on the instrument. Use the Menu screen to exit from, or return to a screen. The Menu screen of the instrument (left) is analogous to the Home screen for the BindIx Software ™...

  • Page 50: Protocol Editor Screen

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Protocol editor screen The protocol editor screen is used to create a new protocol or modify an existing protocol. Create a new protocol. Open an existing protocol. Search for protocol by manually entering a catalog number or using the barcode reader.

  • Page 51: Protocol Library Screen

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Protocol library screen When instrument connected to Internet, users can search and select the available protocols in the Protocol library. Users can also arrange protocols in different orders by selecting either the Name, Application, or Kit SKUs icons.
  • Page 52 Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Set date and time 1. Select (Change) under Date and time 2. Scroll through the numerical menus to select the time and date. 3. Select Set time zone 1.

  • Page 53: Protocol Settings

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Set language 1. Select the Language field to open the drop down menu. 2. Select the desired language (English, Spanish, French, German, Portuguese, Italian, Russian, Japanese, Chinese). 3.
  • Page 54 Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Sound settings Enable sound Switch the toggle to turn instrument sound on or off. Secondary display settings Loading position display setting Switch the toggle to turn the display light under the loading position on or off. Connect settings Automatically export run reports to Connect if signed in Switch the toggle to turn automatic export function on or off.

  • Page 55: Network Share Settings

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Upload telemetry data to cloud Switch the toggle to turn automatic telemetry export function on or off (see “Telemetry data” page 80). Network share settings Network sharing enables the user to export and import protocols or troubleshooting logs to the local network as well as run reports.

  • Page 56: Reset Factory Settings

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls About About ( ) displays instrument information, including instrument serial number, software versions, usage information, IP address, MAC address. and service date. The user can also adjust logout time minutes when in Login access mode.
  • Page 57 Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Download instrument data to troubleshoot 1. Select Troubleshoot. 2. Save troublehsooting report to an USB device or export to local network: • To save the troubleshooting report to a USB, place a USB memory device into a USB device port.

  • Page 58: Instrument Screen

    Not for Clinical Use in China. Chapter 3 Software applications Overview of user interface controls Instrument screen The Instrument screen is the main control panel for manual operation of the instrument. Move the turntable counterclockwise. Insert magnetic head (See “Install magnetic head” page 25).

  • Page 59: Protocol Overview

    Not for Clinical Use in China. Chapter 3 Software applications Protocol overview Protocol overview A protocol is a set of instructions that the instrument uses to perform a sample processing procedure. The protocol is a collection of information regarding the layout with reagent data and procedure steps done in a session as well as the instrument used.

  • Page 60: Enter Protocol Information

    Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Enter protocol information 1. Enter the name of the kit to be used with the protocol in the Kit used field. 2. Select the type of procedure the protocol is used for from the Application type drop down menu. 3.

  • Page 61: Select Magnetic Head

    Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Select magnetic head 1. Select Magnet heads ( 2. Select the checkbox next to the magnetic head(s) to be used in the protocol. One or two magnetic heads can be chosen, but only combinations of compatible heads are possible (i.e., 24-well magnetic heads cannot be combined with 96-well magnetic heads).

  • Page 62: Add Protocol Steps

    Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Add protocol steps 1. Select Protocol steps ( 2. Select Add step to add a step to the protocol. a. Select Define step to define the Step type and enter step parameters. See Step types for details.

  • Page 63: Define Protocol Steps

    Not for Clinical Use in China. Chapter 3 Software applications Create a protocol 7. Select to validate the protocol. The Total time estimate is created for the protocol if it is valid. 8. After completing the protocol, select Save (see “Save a protocol” page 78).

  • Page 64: Step Types

    Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Step types Corresponding plate Icon Step parameters type Step Perform sample lysis without beads. Sample plate • Heating & cooling • Mixing Lysis • Postmix • Precollect beads • Mixing Sample plate •...
  • Page 65 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol (continued) Corresponding plate Icon Step parameters type Step Select tip comb type. Tip plate Use Add tip to add an additional cycle with a new tip comb. You can add as many tip combs to the protocol as necessary.
  • Page 66 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Define plate A plate must be defined for each protocol step. The tip comb gives you a default value. Select the plate type from the drop down menu. • Elution plate •...
  • Page 67 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Precollect beads step Precollect beads is enabled to make the tip comb and the magnetic rods go to the bottom of the well to collect the particles. Use the Precollect beads step if the beads are sedimented at the bottom at the beginning of the step.
  • Page 68 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Toggle to enable bead collection. Set the number of counts. Set the collect time. Heating & Cooling step Heating & Cooling is enabled to heat or cool reagents during a step. Plates can be heated or cooled as many times as necessary during a protocol, but cooling sequences can create condensation.
  • Page 69 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Mix step This step is used to mix beads or reagents. Mixing time [hh:mm:ss] sets the amount of time that the tip oscillates up and down in the reagent in order to mix the reagent with or without the beads.
  • Page 70 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Tip speed There are two types of speeds for the plastic tip movement in the well: full-length speed (Slow, Medium, and Fast) and bottom mix speed (Bottom mix and Half mix). At full-length speeds, the tip moves throughout the length of the well, whereas at bottom mix speeds, the tip moves only at the bottom of the well.
  • Page 71 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Postmix step Postmix is enabled to have the tip (without the magnetic rod) mix the reagent. Postmix is often used after a heating step. Speed is used to select the tip speed for mixing from a drop down menu (Slow, Medium, Fast, Bottom mix, Half mix).
  • Page 72 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Dry step The Dry step dries the magnetic beads above or inside a specific well or tube. During the step the beads remain attached to the tip surface, and the magnetic rod remains inside the tip. The beads can be dried inside the well or tube above the liquid level (Above well) or with the tip completely lifted from the well or tube (Outside well).
  • Page 73 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Guidelines for changing plates The instrument has a turntable with eight plate stations, but a protocol can be created for using more than eight plates by using the following rules. •...
  • Page 74 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Figure 11   Plate change in Protocol Editor KingFisher Apex Purification System User Guide ™...
  • Page 75 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Figure 12   Plate change in Protocol Execution KingFisher Apex Purification System User Guide ™...
  • Page 76 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Figure 13   Plate change in Protocol Editor, Forced Note: The wait step always precedes the forced plate change in protocol tree. When creating the protocol, the user adds the wait step and makes plate change selection available options. KingFisher Apex Purification System User Guide ™...
  • Page 77 Not for Clinical Use in China. Chapter 3 Software applications Create a protocol Release beads step This step releases collected magnetic beads into the wells of the plate. If this step is not added, the magnetic particles remain attached to the tip. Duration [hh:mm:ss] sets the amount of time that the tip oscillates in the reagent in order to release all the beads.

  • Page 78: Save A Protocol

    Not for Clinical Use in China. Chapter 3 Software applications Save a protocol Save a protocol New protocols are saved with the .kfx2 format on the instrument. Older protocol versions with the .kfx format can be run and opened but will be saved in the new format on the insturment. The BindIx ™...

  • Page 79: Export Run Report To A Usb Memory Device

    Not for Clinical Use in China. Chapter 3 Software applications Run history overview For instructions on importing or exporting reports to Connect, see the online help documentation for the Protocol Library. Export run report to a USB memory device 1. Insert a USB memory device into a USB port on the instrument. 2.

  • Page 80: Telemetry Data

    Not for Clinical Use in China. Chapter 3 Software applications Telemetry data Telemetry data Statistical data is collected so that the instrument can be developed to better serve the actual needs of the users. The collected data is not connected to single users, and users do not have access to the statistical data.
  • Page 81 Not for Clinical Use in China. Chapter 3 Software applications Telemetry data • Export count of protocol files [integer] – to USB – to network share – to Connect • Import count of protocol files [integer] – from USB – from network share –...

  • Page 82: Update Software

    Not for Clinical Use in China. Chapter 3 Software applications Update software Update software When the instrument is connected to the internet, a prompt with available software updates appears. See Figure 14. Figure 14   Software update IMPORTANT! Disconnect the instrument from BindIx Software and remove all external equipment ™...

  • Page 83: Appendix A   Troubleshooting

    Not for Clinical Use in China. Troubleshooting Troubleshooting Table 2   Actions taken against error messages and warnings Code Error message Action Switch the instrument OFF and ON, then try again. If the error appears Head position error during initialization or is otherwise repeated, contact service. Switch the instrument OFF and ON, then try again.
  • Page 84 Not for Clinical Use in China. Appendix A Troubleshooting Troubleshooting Table 2 Actions taken against error messages and warnings  (continued) Code Error message Action Cannot do head cycles from Switch the instrument OFF and ON, then try again. If the error appears unknown starting position during initialization or is otherwise repeated, contact service.

  • Page 85: Login Error

    Not for Clinical Use in China. Appendix A Troubleshooting Login error Table 2 Actions taken against error messages and warnings  (continued) Code Error message Action Heaters ambient temperature not Switch the instrument OFF and ON, then try again. If the error appears measured yet during initialization or is otherwise repeated, contact service.
  • Page 86 Not for Clinical Use in China. Appendix A Troubleshooting Login error 2. If login fails repeatably, click Login support screen for service information that is needed to contact local service. Local service provides a passcode that will be needed to reset login credentials.
  • Page 87 Not for Clinical Use in China. Appendix A Troubleshooting Login error Figure 17    Instrument reset screen KingFisher Apex Purification System User Guide ™...

  • Page 88: Appendix B   Maintenance

    Not for Clinical Use in China. Maintenance Regular and preventive maintenance • Preventative maintenance by Service is recommended to be done yearly. There is a service counter which shows estimated next service date. Thirty days before next estimated service date there will be a warning to order service.

  • Page 89: Store The Transport Lock

    Not for Clinical Use in China. Appendix B Maintenance Store the Transport Lock Store the Transport Lock Use the transport lock screws to secure the transport lock to the rear of the instrument. Transport lock plates Transport lock screws Transport lock screw Magnetic head maintenance If necessary, wipe the magnetic rods using a soft cloth or tissue paper soaked in a mild detergent solution (SDS), soap solution, or alcohol.

  • Page 90: Decontaminate Instrument

    Not for Clinical Use in China. Appendix B Maintenance UV decontamination procedure Example of decontaminants: • Ethanol 70% • Virkon solution 1–3% ™ • Glutaraldehyde solution 4% • Chloramine T • Microcide SQ 1:64 ™ • Decon 90 min. 4% ™ Decontaminate instrument 1.

  • Page 91: Uv Treat Process Chamber

    Not for Clinical Use in China. Appendix B Maintenance UV decontamination procedure UV treat process chamber 1. Go to Menu4Instrument4Tools4UV light. 2. Set the decontamination time. Note: The default is 1 h. However, the user can set a default time as needed. The maximum time setting is 23 h 59 min.

  • Page 92: Prepare Instrument For Transport

    Not for Clinical Use in China. Appendix B Maintenance Prepare instrument for transport Prepare instrument for transport If it becomes necessary to relocate or ship the instrument, install the transport lock to secure tip comb holder and magnetic head holder. Refit the transport lock 1.

  • Page 93: Appendix C   Specifications

    Not for Clinical Use in China. Specifications Physical dimensions Parameter Instrument Transport package Width 78 cm (31 in) 99 cm (39 in.) Depth 59 cm (23 in) 77.5 cm (30.5 in.) Height 45 cm (18 in) 83.5 cm (33 in.) Weight 56 kg (123 lb) 58 kg (128 lbs) Weight including instrument. Instrument specifications Technical specifications Operating conditions (indoor use...

  • Page 94: Performance Specifications

    Not for Clinical Use in China. Appendix C Specifications Performance specifications (continued) Technical specifications Ultraviolet light 2 UV lamps 18 W (can be set to run for up to 23h 59 min) Internal barcode reader Performance specifications Table 3   Performance specifications Performance specifications Capacity 96 samples/run max.
  • Page 95 Not for Clinical Use in China. Appendix C Specifications Performance specifications Table 3 Performance specifications  (continued) Performance specifications Tip combs (polypropylene 24- or 96-well format in one frame – disposable, barcoded) • KingFisher Apex 96 Combi tip comb ™ • KingFisher Apex 24 Combi tip comb ™...

  • Page 96: Appendix D   Ordering Information

    Not for Clinical Use in China. Ordering information Accessory products Product Cat. No. Magnetic heads KingFisher Apex 96 PCR Head 24079910 ™ KingFisher Apex 96 Deep Well Head 24079930 ™ KingFisher Apex 96 Combi Head 24079920 ™ KingFisher Apex 24 Combi Head 24079940 ™...
  • Page 97 Not for Clinical Use in China. Appendix D Ordering information Accessory products (continued) Product Cat. No. Plastics for 96 deep well format KingFisher Apex 96 Combi Tip Comb 97002570 ™ KingFisher 96 Deep Well Plate 95040450B ™ KingFisher 96 Deep Well Plate, sterile 95040460B ™...

  • Page 98: Appendix E   Safety

    Not for Clinical Use in China. Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document.

  • Page 99: Symbols On This Instrument

    Not for Clinical Use in China. Appendix E Safety Symbols on this instrument Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information. In this document, the hazard symbol is used along with one of the following user attention words.

  • Page 100: Electromagnetic Compatibility (Emc) Standards

    Not for Clinical Use in China. Appendix E Safety Electromagnetic compatibility (EMC) standards Electromagnetic compatibility (EMC) standards Class B notice This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation.

  • Page 101: Electrical Safety

    Not for Clinical Use in China. Appendix E Safety Electrical safety Electrical safety The following information on electrical safety must be observed, failing to follow these instruction may result in electric shock, fire and/or serious personal injury or death. In the event of an equipment malfunction, it is the responsibility of the customer to report the need for service to Thermo Fisher Scientific or to one of the authorized agents.

  • Page 102: Chemical Safety

    Not for Clinical Use in China. Appendix E Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: ·...

  • Page 103: Biological Hazard Safety

    Not for Clinical Use in China. Appendix E Safety Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases.

  • Page 104: Appendix F   Documentation And Support

    Not for Clinical Use in China. Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •...
  • Page 105 Not for Clinical Use in China. KingFisher Apex Purification System_UG_MAN0018970-v5-GUID-921465A7-3619-4C09-916A- A537AC5F35C7-2024/12/11 15:28:38 en 15:27:57.041-04:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 21 March 2025...

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