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Thermo Scientific King Fisher Flex User Manual

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Thermo Scientific
King Fisher Flex
User Manual
Cat. no. N07669 Rev. B.0 04/ 2021
For Laboratory Use

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Summary of Contents for Thermo Scientific King Fisher Flex

  • Page 1 Thermo Scientific King Fisher Flex User Manual Cat. no. N07669 Rev. B.0 04/ 2021 For Laboratory Use...
  • Page 2 Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07- 06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC.

  • Page 3: About This User Manual

    Chapter Maintenance. For ordering information, refer to Chapter Ordering information. For more For PC software-related issues, refer to the Thermo Scientific™ BindIt™ Software User Manual (N07974). information For the latest information on products and services, visit our websites at: http://www.thermoscientific.com...

  • Page 4: Safety Symbols And Markings

    European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EC. A black label with this text (Figure 4) WARNING: DISCONNECT SUPPLY BEFORE SERVICING AVERTISSEMENT: COUPER L'ALIMENTATION AVANT L'ENTRETIEN ET LE DEPANNAGE Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 5: Warnings And Other Markings Used In Documentation

    • Never force a microplate onto the instrument. • The KingFisher Flex is intended for laboratory use only. Observe proper laboratory safety precautions, such as wearing protective clothing and obeying approved laboratory safety procedures. Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual...
  • Page 6 Warning If the device is not used according to manufacturer’s instructions the protection provided by the device cannot be guaranteed. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 7: Table Of Contents

    How to fit the subassemblies of the instrument into place .. 23 Shield plate ............... 23 Heating block ..............25 Operational check ..............31 How to pack the instrument for transportation ....32 Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 8 Performance specifications ..........67 Safety specifications.............67 In conformity with the requirements ........67 Chapter 7 Frequently asked questions ............70 70 Chapter 8 Troubleshooting guide ..............74 Error messages and warnings ..........74 Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 9 Contents Troubleshooting guide ............75 Chapter 9 Ordering information ..............77 KingFisher Flex ..............77 List of accessories and consumables ........77 List of spare parts..............78 Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 10: Introduction To The Kingfisher Flex

    The protocol that is selected by the user via the keypad and display has already been preloaded into the onboard software. Thermo Scientific™ BindIt™ Software can be used to create and run protocols. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 11: Advantages Of Using Kingfisher Flex

    • No carryover • No cross-contamination • Facilitates the whole processing with the aid of an internal program/BindIt Software. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 12: Functional Description

    Figure 2 and Figure 3. Figure 2. KingFisher Flex front view without transparent lid Loading station Interchangeable KingFisher Flex head Keypad Shield plate Display Plate station (1-8) Heating block Turntable Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 13: Back / Side View

    Figure 4 and the side view in Figure 5. Figure 4. KingFisher Flex back view Cooling air inlet Warning marking Type label Mains power supply connector USB port ON/OFF switch Serial port RS-232C Cooling air outlet Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 14: Kingfisher Flex Magnetic Particle Processor

    Magnetic particles are transferred with the aid of magnetic rods covered with a disposable, specially designed plastic tip comb. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 15: Principle Of Magnetic Particle Processing

    The magnetic rods together with the particles tip comb move slowly up and down in the plate and the magnetic particles are collected onto the edge of the tips. The Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 16: Releasing Magnetic Particles

    The volume of the first plate can be larger than the volume of the next plate, and this is used for concentration purposes volume during the (refer to Figure 8). magnetic particle processing Figure 8. A concentration step during magnetic particle processing Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 17: Chapter 3 Installation

    Examine the Examine the enclosed packing list against order. In case of any deviations, contact your local Thermo Fisher Scientific delivery representative or Thermo Fisher Scientific. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 18: Requirements

    Place the instrument on a normal laboratory bench. The net weight of the entire equipment is approx. 28 kg [62 lbs.]. The instrument operates at voltages of 100–240 Vac and the frequency range of 50/60 Hz. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 19: Precautions And Limitations

    Remove both the transport locks. Make sure both the transport locks are released before you put the instrument into operation. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 20 1. To remove the transport lock of the tip comb holder, take hold of the transport lock screw and firmly turn it counterclockwise according to Figure 10. Figure 10. Remove the transport lock of the tip comb holder Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 21 (Figure 12). Lift the screw with the label attached (Figure 13). Figure 12. Remove the transport lock of the heating block (A) 4. Remove the transport lock screw from the label. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 22: How To Ensure Startup

    (Figure 4) corresponds to the local voltage. 2. Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 23: How To Fit The Subassemblies Of The Instrument Into Place

    Use a hexagonal screwdriver (Allen key) to fasten the two screws (Figure 15). Figure 15. Shield plate prior to fitting 3. First insert the shield plate into its slot (Figure 16). Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 24 4. When you fasten the two plate retaining screws (Figure 17), be careful not to drop them inside the instrument. Figure 18 shows the shield plate installed. Figure 17. Fasten / loosen the shield plate Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 25: Heating Block

    3. Insert the heating block by first setting it and then pressing it hard into place (Figure 19 and Figure 20). The heating block snaps into place at both ends if you have done it correctly. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 26 Figure 20. Press the heating block into place You can add a heating step of ambient temperature +5°C to +115°C to a protocol. The heating block is preheatable, but no Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 27 Caution The heating block is specifically designed for the plates listed below to ensure even heating during the sample process (Figure 21). Using other plates than those recommended may damage the instrument and diminish the application performance. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 28 KingFisher Flex heads away from each other, and other magnets at all time. Interference of the magnets on one another can cause serious damage to the magnets. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 29 KingFisher Flex heads close to a PC display, since this may cause damage to the display. Do not use metal tools when you handle KingFisher Flex heads. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 30 Flex head, be careful not to damage the magnet rods against the tip comb holder frame. Confirm the end of the protocol by pressing START. Figure 23. Insert the KingFisher Flex head Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 31: Operational Check

    First set the instrument ON. The instrument performs initialization tests and adjustments. check The display quickly shows the internal software version. This happens when the initialization tests and adjustments have been completed. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 32: How To Pack The Instrument For Transportation

    4. Insert the transport lock screw into the label and refit the transport lock of the heating block into its place (Figure 26). Figure 26. Refit the transport lock of the heating block (A) Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 33: How To Refit The Transport Lock Of The Tip Comb Holder

    1. Press the tip comb holder downwards evenly with both hands (Figure 28). Caution Make sure that the power is set to off. Figure 28. Press the tip comb holder downwards Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 34 Note One of the holes is inside the instrument and not clearly visible. 3. Fasten the screw by turning it firmly clockwise. Figure 29. Location of the locking hole 1. Locking hole Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 35 Figure 30. Fit the transport lock of the tip comb holder Figure 31. Both transport locks refitted Figure 31 shows both the transport lock of the heating block and the tip comb holder refitted. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 36: Chapter 4 Routine Operation

    PC and the instrument are set to off. • Do not use the instrument if it appears that it does not function properly. • Do not under any circumstances use formaldehyde. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 37: Set On

    COUNTERCLOCKWISE buttons The relevant keys and control buttons are described in detail Keys below. The arrow keys are used to select the next protocol and to navigate in the display. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 38: Display

    Figure 33. Main view on the display of the KingFisher Flex Menu Icon Main view row Info text bar There are four menus in the KingFisher Flex user interface: Factory protocols, User protocols, Maintenance, and Settings. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 39: Navigation

    Left and Right arrow keys. The main views of each menu tab are shown on Table 3Error! R eference source not found.. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 40 Routine operation Table 3. Main views Factory protocols User protocols Maintenance Settings Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 41: Use Of Kingfisher Flex Pc Software

    Factory protocols are protocols which are preloaded into the instrument internal memory in the factory, for example, protocols demo protocols. User protocols are protocols which are made and transferred using BindIt Software. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 42: Run A Protocol

    The example shows demo_Flex96 protocol. 1. Press START to run the protocol. Follow the instructions on the screen. 2. First load the Tip plate. 3. Press START to continue. Add the elution buffer. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 43 4. Press START to continue. Load the wash buffer. 5. Press START to continue. Load the buffer and beads. 6. Press START to continue. The screen tells you how much time remains before the protocol finish. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 44: Set The Default Protocol

    Go to the Last used/Default protocol row in Factory protocols / User protocols menu. protocol The last protocol you used is shown. Press OK to set your default protocol. Maintenance Go to the Maintenance menu. protocol Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 45: Instrument Options

    Device information The device information shows the name of the instrument, the internal software version and the serial number of the instrument unit. Go to the Settings menu. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 46: Computer Interface

    Use the Up and Down arrow keys to select the language in use. The available languages are English, German, French, Spanish and Portuguese. The default language is English. Press OK. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 47: Sound

    Figure 34. Tip comb package with two tip combs 2. Open the package and separate the two tip combs (Figure 35). Place one of the tip combs onto the KingFisher 96 plate (= tip-plate). Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 48 Figure 36. Store the other tip comb on a KingFisher 96 plate for further use Do this to handle PCR tip combs correctly. 1. Take a PCR tip comb package containing eight tip combs within each other on a screen plate. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 49 3. Before you take the tip comb into use, press the tip comb evenly against the enclosed 24 deep well plate with the aid of a magnetic head or another object. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 50: How To Start

    Place only one tip comb onto a KingFisher plate (= tip-plate) per run (Figure 40). Press START to confirm the plate loading. Figure 39. Combine the tip comb and KingFisher plate (A) Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 51 The plate station position depends on the protocol used. After the protocol has been run, note that the turntable may stop in a different position than the basic position. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 52 Routine operation Figure 41. Load the tip-plate A1 corner Figure 42. Load the plates Tip plate Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 53 Routine operation Figure 43. Plates loaded Tip comb holder Magnetic rods Tip plate 4. The tip comb is automatically locked onto the tip comb holder from the tip-plate (Figure 44). Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 54 Tip comb in the tip comb holder 5. When the turntable moves, the shield plate moves over the plate situated underneath (Figure 45) forming a protective cover. Figure 45. Shield plate in function Shield plate Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 55 24 deep well plate KingFisher Flex head for – 50–1000 µl 50–150 µl – Microtiter deep well 96 plate KingFisher Flex head for KF – – 20–200 µl – 96 plate Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 56: Change The Heating Block

    The heating block will snap into place at both ends if you have done it correctly. 5. Press the START key when the task has been completed. Figure 47. Insert the heating block Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 57 24 deep well plate Heating block for Microtiter – – – deep well 96 plate Heating block for KingFisher – – 96 plate Heating block for PCR plate – – – Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 58: Shutdown

    3. Carry out appropriate corrective measures. However, do not disassemble the instrument. 4. If the corrective measures taken do not help, contact authorized technical service or your local Thermo Fisher Scientific representative. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 59: Chapter 5 Maintenance

    Warning If any surfaces are contaminated with biohazardous material, a mild sterilizing solution should be used. Caution Do not autoclave any part of this instrument except the shield plate. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 60: How To Clean The Turntable

    Unscrew the two finger screws. Note that the screws remain attached to the turntable when the screws are unfastened. Figure 50. Unfasten the two finger screws 1. Alignment stud Lift the turntable off. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 61 Figure 52. Black spill shield exposed for cleaning 1. Alignment stud When you replace the turntable, insert the turntable so that you place it first onto the aligning stud. Then fasten the two finger screws. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 62: Disposal Of Materials

    If you have spilled infectious agents, carry out the decontamination procedure. n procedure Warning The decontamination procedure should be performed by authorized trained personnel in a well-ventilated room wearing disposable gloves, protective glasses and clothing. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 63 7. Close the bag firmly and leave the instrument in the bag for at least 24 hours. 8. Remove the instrument from the bag. 9. Clean the instrument using a mild detergent. 10. Remove any stains using 70% ethanol. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 64: Pack For Service

    If the KingFisher Flex must be discarded, obey the guidelines below. instrument Warning Decontaminate the instrument before disposal. Refer to Decontamination procedure about decontamination. Obey laboratory and country-specific procedures for biohazardous or radioactive waste disposal. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 65 Electronic waste Contaminated waste (Infectious waste) Regarding the original packaging and packing materials, use the recycling operators known to you. For more information, contact your local Thermo Fisher Scientific representative. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 66: Chapter 6 Technical Specifications

    Character format 1 start bit, 8 data bits, 1 stop bit, and no parity. Flow control XON/XOFF. Internal software Space for max. 512 internal protocols Normal use 10 runs/day, 250 days/year, with 40 min protocols having a medium speed setting, RT Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 67: Performance Specifications

    This section describes the safety specifications for the KingFisher Flex instrument. specifications In conformity with KingFisher Flex bears these markings: the requirements Type 711 100–240 Vac, 50/60 Hz, 175 VA CE mark NRTL certification for US/CA Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 68 2500 V for a 230 V supply and 1500 V for a 120 V supply. Note 2 The pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only nonconductive Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 69 Technical specifications pollution, such as dust, occurs except for occasional conductivity caused by condensation. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 70: Frequently Asked Questions

    Caution It is very important to keep the KingFisher Flex heads away from each other and other magnets at all times. Interference of the magnets on one another may cause serious damage to the magnets. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 71 80°C? A9: The heating block warms up about 10 degrees per minute, so it will take about 6 minutes. Q10: What should I do if the magnets set are dirty? Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 72 Q16: Is it always compulsory to use the transport locks? A16: The transport locks are only necessary when relocating the instrument. Q17: What should you do if the tip loading fails? Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 73 Frequently asked questions A17: Try first to manually stretch the tips both lengthwise and width wise to level the tip comb. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 74: Troubleshooting Guide

    However, this error may show up in the error log. The requested movement is not Cannot perform the requested movement. For example, the allowed head and the magnets cannot move down when the shield Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 75: Troubleshooting Guide

    Set the instrument to OFF and ON and try again. If the error mechanism is out of position appears during initialization or is otherwise repeated, contact service. Serial number already set Contact authorized technical service Invalid command parameter Contact authorized technical service. Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...
  • Page 76 It is recommended that you cleanup the program memory from unnecessary protocols about once a month to prevent this error from occurring. Code Warning Description Timer expired Contact authorized technical service. Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...

  • Page 77: Ordering Information

    KingFisher Flex 96 tip comb for PCR magnets 80 pcs 97002524 KingFisher Flex 96 tip comb for KF 96 magnets 100 pcs 97002534 KingFisher Flex 96 tip comb for DW magnets 100 pcs Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

  • Page 78: List Of Spare Parts

    Table 13. Codes for spare parts parts Code Item Quantity 2305290 Serial cable F9/F25 (for RS-232C port) N04001 USB A-B device cable 1.8 m* * Longer USB cables available from PC stores Thermo Scientific King Fisher Flex User Manual Thermo Fisher Scientific...
  • Page 79 Thermo Fisher Scientific Thermo Scientific King Fisher Flex User Manual...

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