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Fig. 3
Adjusting the
Aperture
The aperture iris diaphragm adjusts image resolution and contrast.
For fluorescence observation, push in the aperture iris diaphragm lever ③.
Both ND (neutral density) filter and small aperture diaphragm can help weaken the intensity of the
excitation light to delay color fading of the specimen
According to the objective in use, adjust the diaphragm image using the field iris diaphragm lever ③ so
that the field of view is circumscribed by the field iris diaphragm to exclude stray light.
ACCU-SCOPE
EXC-500 FLUORESCENCE MICROSCOPE SYSTEM
Iris Diaphragm (Fig. 3)
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
Centering the
Aperture
(Fig. 3)
Switch the light shutter ① to "●"close position to
turn off the light path.
Rotate the filter block turret to engage the G-
excitation mirror block or another into the light
path.
Switch the light shutter ① to"O" open position
to open the light path.
Rotate the 10× objective in the light path, and
place the centering plate (a white plate with a
cross) on the stage and bring into approximate
focus.
Move the cross of the centering plate to the
center of the field of view.
Pull out the aperture diaphragm lever ② to
adjust the aperture iris diaphragm to the
smallest diameter.
Pull out the field iris diaphragm lever ③ to adjust
the field iris diaphragm to the smallest diameter.
The image of aperture iris diaphragm can be
found on the centering plate.
Adjust the aperture iris diaphragm centering
screws ④ with attached wrench to superpose
the image of aperture iris diaphragm on the
cross of centering plate.
Iris Diaphragm
10
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