Leica VT1200 Instructions For Use Manual
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Leica VT1200 Instructions For Use Manual

Vibrating-blade microtome
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Instructions for Use
VT1200 / VT1200 S
Vibrating-blade
microtome
Leica VT1200 / VT1200 S V 1.5, English - 07/2016
Order No. 14 0481 80101 RevH
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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Summary of Contents for Leica VT1200

  • Page 1 Instructions for Use VT1200 / VT1200 S Vibrating-blade microtome Leica VT1200 / VT1200 S V 1.5, English - 07/2016 Order No. 14 0481 80101 RevH Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 Leica reserves the right to change technical following thorough investigation in this field. We specifications, as well as manufacturing pro- are under no obligation to update the present cesses, without prior notice.

  • Page 4: Table Of Contents

    5.4.4 Installing and removing the blade holder ................34 5.4.5 Inserting the blade ........................35 5.4.6 Adjusting the clearance angle ....................35 Routine daily maintenance and switching off the instrument – VT1200/VT1200 S ..36 Instructions for Use V 1.5 RevH – 07/2016...
  • Page 5 Contents Operating the VibroCheck ...................... 37 Using the VibroCheck with the VT1200 ................... 37 Using the VibroCheck with the VT1200 S ................39 Malfunctions: Meaning and Troubleshooting ..............41 Error messages and troubleshooting ..................41 Replacing the main fuse ......................46 Cleaning and Maintenance ....................

  • Page 6: Important Information

    Important Information Symbols in the text and their meanings Environmental protection symbol of the China RoHS directive. Warnings The number in the symbol indicates appear in a gray box and are marked by the "Environment-friendly Use Period" a warning triangle of the product in years.

  • Page 7: Qualification Of Personnel

    Intended use/improper use The Leica VT1200 and VT1200 S are used for sectioning in the fields of medi- cine, biology and industry, and are especially designed for sectioning fixed or unfixed fresh tissue in buffer.

  • Page 8: Safety

    Safety These Instructions for Use includes important information related to the operating safety and maintenance of the instrument. The Operating Manual is an important part of the product, and must be read carefully prior to startup and use and must always be kept near the instrument.

  • Page 9: Transport, Unpacking And Setting Up

    The instrument must be set up so that the main power switch on its right side (item 7 in Fig. 14) is easily accessible at any time. Because the weight of the instrument is approx. 56 kg, carrying the instrument requires 2 persons (1 carrying handle per person). Leica VT1200 / VT1200 S...

  • Page 10: Instrument Characteristics

    Instrument characteristics Technical data for the VT1200 General data: Sectioning frequency (±10 %) ......................85 Hz (± 10 %) Amplitude....................from 0 - 3 mm, in 0.05 mm increments Sectioning speed (± 10 %) ........................ 0.01 - 1.5 mm/s Return speed (± 10 %) ..........................2.5 mm/s Total vertical specimen stroke ....................20 mm (motorized)

  • Page 11: Technical Data For The Vt1200 S

    Instrument Characteristics 3.1.1 Technical data for the VT1200 S General data: Sectioning frequency (± 10 %): ........................85 Hz (± 10 %) Amplitude: ....................... from 0 - 3 mm, in increments of 0.05 mm Sectioning speed (± 10 %): ........................0.01 - 1.5 mm/s Return speed (±...

  • Page 12: General Overview - Vt1200/Vt1200 S

    Instrument characteristics General overview – VT1200/VT1200 S Fig. 4, Microscope Fig. 3, Magnifier Fiber optic light guide Cold light source Fig. 5 Cutting head Blade holder Fig. 2, Basic instrument Fig. 8, Control panelm VT1200 Fig. 7, VT1200 control Fig. 6,...
  • Page 13 1 c m in height in height Fig. 9 for specimens 2 cm for specimens 2 cm in height in height Blades for the blade holder Sapphire blade Directional Directional Cyanoacrylate adhesive Cyanoacrylate adhesive Leica VT1200 / VT1200 S...

  • Page 14: Vt1200 Control Panel

    LED lights up. The Leica VT1200 is a semiautomatic microtome with a vibrating blade. Before each cut, a man- ual feed to the desired section thickness must be carried out using the setting dial for section thickness.

  • Page 15: Vt1200 S Control Panel

    (MAN) sectioning mode (SINGLE) is possible. The Leica VT1200 S is a fully automatic microtome with vibrating blade. It can be operated in automatic as well as semiautomatic sectioning mode. In semiautomatic sectioning mode, a manual feed to the desired section thickness must be carried out before each cut.

  • Page 16: Installation

    Installation Standard scope of delivery for the VT1200 VT1200 basic instrument ............... 14 0481 42065 1 control panel ................14 0481 43395 1 toolset: - 1 Allen key, size 3.0 ..............14 0194 04764 - 1 Allen key, size 6.0 ..............14 0222 04141 - 1 cryo-manipulator ..............

  • Page 17: Standard Scope Of Delivery For The Vt1200 S

    Installation 4.1.1 Standard scope of delivery for the VT1200 S VT1200 S basic instrument ............. 14 0481 42066 1 control panel ................14 0481 43396 1 toolset: - 1 Allen key, size 3.0 ..............14 0194 04764 - 1 Allen key, size 6.0 ..............14 0222 04141 - 1 cryo-manipulator ..............

  • Page 18: Packing And Setting Up The Instrument

    Installation Packing and setting up the instrument Ensure that the instrument is standing on a work surface that is as free of vibrations as possible. Before each transport, the handles must be screwed onto the instrument using the provided screws (see Fig.

  • Page 19: Before Commissioning The Instrument

    7. Use the power switch (7) to switch on the instrument. To make it easier to insert the speci- men, adjust the specimen receptacle to its lowest position, and adjust the blade holder to its rearmost position during the first reference run. Fig. 14 Leica VT1200 / VT1200 S...

  • Page 20: Working With The Instrument

    Working with the Instrument Description of the typical application The Leica VT1200 / VT1200 S is a microtome with a vibrating blade and is predominately used for sectioning fixed and unfixed specimens during neurological research. • To simplify the process of inserting the speci-...

  • Page 21: Control Elements On The Vt1200 Control Panel

    Working with the Instrument Control elements on the VT1200 control panel Caution: To practice the key functions, always make sure there are no blades installed! Do not install the blades until you are familiar with all of the key functions.

  • Page 22: Fine-Adjusting The Blade-To-Specimen Proximity And Feeding To The Section Thickness

    Working with the Instrument 5.2.2 Fine-adjusting the blade-to-specimen proximity and feeding to the section thickness The rotary knob 2 for moving the specimen receptacle vertically can be used both for fine-adjusting the blade-to-specimen proximity and for adjust- ing the desired section thicknesses. The step size: 1, 10 or 100 µm can be selected by pressing the STEP SIZE key.

  • Page 23: Selecting The Sectioning Parameters

    "Blade back" or "Blade forward" key. The µm display is then reset to "0". To start a new sectioning process, use the "Blade back" key to move the blade to the beginning of the specimen, set the desired section thickness and restart the sectioning process. Leica VT1200 / VT1200 S...

  • Page 24: Control Elements On The Vt1200 S Control Panel

    Working with the Instrument Control elements on the VT1200 S control panel The Leica VT1200 S is a fully automatic microtome with a vibrating blade that can be operated in semiautomatic or automatic sectioning mode. Key / setting dial Semiautomatic sectioning mode Automatic sectioning mode...
  • Page 25 The following functions keys be- Toggling from MAN to when these keys are pressed. come enabled again in automatic AUTO mode: • Cutting window edges that have already been set • Section thickness (AUTO FEED) and continuous stroke (CONT) Leica VT1200 / VT1200 S...
  • Page 26 Working with the Instrument Key / setting dial Semiautomatic sectioning mode Automatic sectioning mode The blade feed rate can be ad- ditto justed from 0.01 to 1.5 mm/s: 0.01 - 0.1 in 0.01 mm/s increments, 0.10 - 0.5 in 0.02 mm/s increments, 0.50 - 1.5 in 0.10 mm/s increments.
  • Page 27 When the DOWN key is pressed, ditto the specimen receptacle auto- matically moves quickly into the lowest position. (The LED in the DOWN key lights up when the lower end position is reached.) The ∑µm display is set to "0". Leica VT1200 / VT1200 S...
  • Page 28 Working with the Instrument Key / setting dial Semiautomatic sectioning mode Automatic sectioning mode If the DOWN key is pressed a ditto second time while the specimen receptacle is moving downwards, the specimen receptacle comes to a stop and the current posi- tion appears on the ∑µm display (lowest position = 0, uppermost position = 20,000 µm).
  • Page 29 LED in the key lights up. Not possible. Advance the blade to the end of the specimen using the "Blade forward" key and press "2nd cutting window edge" until the LED in the key lights up. Leica VT1200 / VT1200 S...
  • Page 30 Working with the Instrument Key / setting dial Semiautomatic sectioning mode Automatic sectioning mode Only single stroke (SINGLE) is pos- Toggles between single (SINGLE) sible. If an attempt is made to switch and continuous stroke (CONT). to continuous stroke (CONT), an The corresponding LED lights up audible warning signal sounds.
  • Page 31 If the AUTO FEED key is pressed, one feed motion takes place according to the value programmed in automatic mode. If the key is pressed more than once, several feed motions take place. Leica VT1200 / VT1200 S...
  • Page 32 Working with the Instrument Key / setting dial Semiautomatic sectioning mode Automatic sectioning mode  Rotary knob 2  Rotary knob 2 Mode: Choice between AUTO Mode: Choice between AUTO and and MAN with rotary knob 1; for MAN with rotary knob 1; for the semiautomatic sectioning mode, automatic sectioning mode, AUTO MAN must be selected.
  • Page 33 If you want to call up the parameters of a certain user (for example, user 3), press the Menu key and select user 3. Then confirm by pressing the Menu key 2x. The parameters stored under user 3 are now enabled. Leica VT1200 / VT1200 S...

  • Page 34: Installing The Accessories

    Working with the Instrument Installing the accessories 5.4.1 Installing the ice tray and buffer tray • A lever (2), which needs to be pushed forward, is located on the underside of the ice tray (1). • Now push the tray onto the dovetail holder (3) from the front.

  • Page 35: Installing The Double-Walled Buffer Tray

    • Screw the manipulator (6) onto the specimen plate, place it in the buffer tray and adjust it to the desired position. • The specimen plate is held in place in the buf- fer tray magnetically. Fig. 18 Leica VT1200 / VT1200 S...

  • Page 36: Installing And Removing The Blade Holder

    Working with the Instrument 5.4.4 Installing and removing the blade holder Always remove the blade BEFORE in- stalling or removing the blade holder! For quality and service reasons, the blade holder (7) is available as a com- plete unit only. •...

  • Page 37: Inserting The Blade

    (8) and rotate it to the desired clearance angle mark. 12 - 15° 13 - 18° 14 - 21° For 15°, the effective clearance angle is "0". The most commonly used setting is 18° (13). Fig. 22 Leica VT1200 / VT1200 S...

  • Page 38: Routine Daily Maintenance And Switching Off The Instrument - Vt1200/Vt1200 S

    Working with the Instrument Routine daily maintenance and switching off the instrument – VT1200/VT1200 S After all daily procedures have been finished, perform the following: • Switch off the main switch on the side of the instrument. • Place the magnifier cover on the magnifier.

  • Page 39: Operating The Vibrocheck

    Operating the VibroCheck Using the VibroCheck with the VT1200 The following instructions must be adhered to exactly. Noncompliance can cause serious damage to the instrument. We recommend using the VibroCheck after each blade change to check the optimum position of the blade and minimize the vertical vibration.
  • Page 40 Operating the VibroCheck When LED on VC is green and LED in RUN/ STOP is yellow, the blade starts to vibrate with the amplitude set. Speed = 0, amplitude can be changed at any time. On the (5-digit) display, the deviation in height amplitude is shown in µm (e.g.

  • Page 41: Using The Vibrocheck With The Vt1200 S

    Operating the VibroCheck Using the VibroCheck with the VT1200 S The following instructions must be adhered to exactly. Noncompliance can cause seri- ous damage to the instrument. We recommend using the VibroCheck after each blade change for checking the optimum posi- tion of the blade and important sectioning parameters.
  • Page 42 Operating the VibroCheck The deviation of the height amplitude is shown on the display in µm (1). This value can be ei- ther positive or negative. A number (e.g. –0.3) appears in the ∑µm display (2). This means a counterclockwise rotation (because of the "-") by a turn of 0.3 and reduces the height ampli- tude to a minimum.

  • Page 43: Malfunctions: Meaning And Troubleshooting

    - Inform service! not be moved away from. Error 26 - "X-Stop" limit switch is not - Inform service! reached. After all error messages, the instrument MUST be switched off, then on again using the main switch. Leica VT1200 / VT1200 S...
  • Page 44 Malfunctions: Meaning and Troubleshooting Error No. / Error Troubleshooting Comment INF No. Error 27 - "Bottom" limit switch of - Check whether an ob- the Z-axis is not reached stacle is blocking the during initialization or travel path of the ice tray. operation.
  • Page 45 Use a new blade present before a blade or blade holder. calibration is canceled - Controllers have different automatically. software versions. Have the software updated. - VibroCheck is defective. Have it checked by ser- vice. Leica VT1200 / VT1200 S...
  • Page 46 VibroCheck. - VibroCheck is defective. - Inform service! - VibroCheck blade search - No blade and/or blade - VT1200 drives to the low- Error 53 not successful. holder swung out or not est Z position using the installed.
  • Page 47 VT connection cable: Check is not working. loose or the instrument is the control unit and power connected to the power plug. supply improperly or not at Replace the power fuses. all. The power fuses are defec- tive. Leica VT1200 / VT1200 S...

  • Page 48: Replacing The Main Fuse

    Malfunctions: Meaning and Troubleshooting Replacing the main fuse Caution: Always unplug the power supply before replacing the fuses! • Remove the fuse housing on the right side of the instrument above the power switch. To do so, push an appropriate tool (small screwdriver) into the slots on the left and right (Fig.

  • Page 49: Cleaning And Maintenance

    Cleaning the blades Always wipe the blade from the back of the blade (sapphire blade) to the cutting edge. NEVER wipe in the opposite direction - risk of injury! Clean using an alcohol-based solution or acetone. Leica VT1200 / VT1200 S...

  • Page 50: Ordering Information For Optional Accessories, Consumables And Spare Parts

    Ordering Information for Optional Accessories, Consumables and Spare Parts Ice tray ......................14 0481 42010 Buffer tray Buffer tray, assembly (plastic) ..............14 0481 42089 Buffer tray, assembly (metal) ..............14 0481 42084 Buffer tray, double-walled, assembly ............14 0481 44837 Lids for covering the buffer trays made of plastic or metal ....................

  • Page 51: Extension Accessories For Standard Specimens

    Ice tray • Removable • Drawer design • Remains stable on the stage when preparing specimen • Integrated hand rests for use with Leica VT1200/VT1200 S. Order No..........14 0481 42010 Fig. 36 9.1.1 Buffer trays Buffer tray, assembly (plastic) •...

  • Page 52: Specimen Plate

    Ordering Information for Optional Accessories, Consumables and Spare Parts Buffer tray, double-walled, incl. integrated hand rest, assembly • Buffer tray, double-walled • Magnetic holder for the specimen plate • Specimen plate, non-directional • Clamp for holding the hose in position in the buffer tray •...

  • Page 53: Vibrocheck

    Can be adjusted using the adjusting screw on the blade holder. Order No..........14 0481 42075 Fig. 44 Leica VT1200 / VT1200 S...

  • Page 54: Blades

    Ordering Information for Optional Accessories, Consumables and Spare Parts 9.1.4 Blades Sapphire blade, knife angle of 22° The blade can be reground. Order no..........14 0216 39372 Fig. 45 9.1.5 Microscope, assembly - Microscope support - S6 Stereozoom microscope - 2 eyepieces, 10x23 B, adjustable - Protective objective glass - Bearing cylinder with 3 screws - Dust cover, large...

  • Page 55: Installing The Magnifier Support Or Microscope Support

    • The microscope height can be adjusted using the setting dials for height adjustment (7, Fig. 50a) and adapted to the respective specimen. Fig. 49 Adapter for installa- tion of a fiber-optic light guide Fig. 50b Fig. 50a Leica VT1200 / VT1200 S...

  • Page 56: Fiber-Optic Illumination, Cold Light Source

    Fig. 52 Order no. 14 0502 30028 Leica CLS 100 cold light source • Serves as a light source for the fiber-optic illumination. 100-120 V, 50/60 Hz Order No. 14 0502 30214 230-240 V, 50/60 Hz Order No. 14 0502 30215 240 V, 50/60 Hz Order No.

  • Page 57: Cyanoacrylate Adhesive

    Fig. 54 9.1.11 Julabo FL300 – recirculating cooler/chiller Recirculating cooler/chiller for connecting to the double-walled buffer tray in the Leica VT1000 S and VT1200/VT1200 S. Selectable temperature range: –20 °C to +40 °C. Recommended cooling medium: Antifrogen N (14 0481 45443)

  • Page 58: Warranty And Service

    Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted.

  • Page 59: Decontamination Certificate (Master)

    Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
  • Page 60 Such equipment must not be returned without the written agreement of Leica Biosystems. Decontamination Certificate (Master) The equipment has been prepared to ensure safe handling/transportation. Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer.
  • Page 62 Leica Biosystems Nussloch GmbH Heidelberger Straße 17-19 D- 69226 Nussloch Phone: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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