Leica EM CPD300 Operating Manual
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Leica EM CPD300 Operating Manual

Critical point dryer
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From Eye to Insight
OPERATING MANUAL
EM CPD300
167180032 Version 12/18

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Summary of Contents for Leica EM CPD300

  • Page 1 From Eye to Insight OPERATING MANUAL EM CPD300 167180032 Version 12/18...
  • Page 2 Important Note Leica Mikrosysteme GmbH reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to con- tinuously improve the technology and manufacturing techniques used to provide our customers with excellent products.
  • Page 3 Leica EM CPD300 Operating Manual Leica EM CPD300 Serial Number: Date of purchase: For the instrument serial number, please refer to the name type label on the back of the instrument! Please read this instruction manual carefully before operating the instrument.
  • Page 4 Foreword This technical documentation is intended to provide essential information about the proper operation and servicing of the EM CPD300 critical point dryer. Service and operating staff must familiarize themselves with all components of the system before commissioning. Particular attention must be paid to the aspect of safety.

  • Page 5: Table Of Contents

    7.6 Preset of Cooling Temperature of the Sample Chamber ..........29 7.6.1 CO -Bottle Temperature / Pressure Function ............. 29 7.7 Starting the EM CPD300 auto procedure ................. 30 7.8 Time and timer function ..................... 30 8. Manual Operation of EM CPD300 ..................35 8.1 Screen descriptions ......................
  • Page 6 15. Service Notes .......................... 46 15.1 Gas-Out filter maintenance ....................46 15.2 General maintenance ......................47 16. User Maintenance ........................48 16.1 Poral filter replacement ..................... 48 16.2 Cleaning ..........................50 17. Warnings and Troubleshooting .................... 51 Page 3 Operating Manual EM CPD300 12/18...

  • Page 7: Introduction

    Introduction In order to ensure the safety of service technicians and operators, and to prevent any damage to the EM CPD300 critical point dryer, it is essential to carefully read this manual before beginning any work with the system. This Operating Manual is intended to help the user understand the system more completely, to use it within the specified limits of its working capabilities, and to maintain in accordance with its physical parameters.

  • Page 8: Safety

    Safety Warning! The EM CPD300 critical point dryer can be operated simply and safely, if the system is operated according to the operating manual. Safety concept of Leica Mikrosysteme GmbH The use in accordance with regulations of the EM CPD300 critical point dryer consists of critical point drying of biological or industrial samples with minimal deformation of the specimens for subsequent SEM analysis.
  • Page 9 General safety regulations Generally, the following safety regulations apply to the handling of the EM CPD300 critical point dryer:  Every user is responsible for her / his own health.  Only instructed users, authorized by the customer are allowed to work at or with the system.

  • Page 10: Installation And Warranty

    -Container is not equipped with a residual pressure valve. In order to prevent accidents, the CO -pressurized gas container must be securely fastened in an upright position. Screw the high-pressure gas hose onto the CO IN connection. Page 7 Operating Manual EM CPD300 12/18...
  • Page 11 Connect the other end of the high-pressure gas hose to the gas pressure container. The high-pressure gas hose and seal are included in the accessory set of the unit. Electrical Connection Check assigned connecting voltage of the unit. Connect power cable to wall socket. Page 8 Operating Manual EM CPD300 12/18...

  • Page 12: Warranty

    Warranty The EM CPD300 is covered by a WARRANTY in accordance with the conditions of sale. If functional errors should occur or if the components of the system sustain damage that is subject to warranty coverage during the warranty period, the manufacturer will repair or replace the faulty components following examination thereof.

  • Page 13: Method

    T (°C) = Critical Point = Triple Point A = Air drying (phase boundary crossing) B and C = Critical point drying (no phase boundary crossing) Page 10 Operating Manual EM CPD300 12/18...
  • Page 14 (C). After coating, the dried sample can be analyzed in a scanning electron microscope (SEM). Comparison between air and critical point drying: Critical point dried sample (Water flea) Air dried sample (Water flea) Page 11 Operating Manual EM CPD300 12/18...

  • Page 15: Description And Specification

    Critical point drying with the EM CPD300 is the optimal method to prevent the damaging effects of the above-mentioned forces by bringing the liquid to the gas phase without boundary crossing.

  • Page 16: Preparation Of Specimens

    Fix chemically with a suitable fixing agent. Wash out fixing agent with a suitable buffer solution. Dehydrate the chemically fixed specimen with acetone or ethanol. (Protocol examples are shown in the EM CPD300 Application booklet). Page 13 Operating Manual EM CPD300 12/18...

  • Page 17: Automatic Operation Of Em Cpd300

    Automatic Operation of EM CPD300 Screen descriptions Initialization screen: Touch screen after initialization. Main Screen: Dark gray buttons can be activated, light gray buttons are inactive! Page 14 Operating Manual EM CPD300 12/18...
  • Page 18 Programmable under programs. 12 Status display gas out speed. Programmable under programs. 13 Process start. (after defining program). 14 Timer function (see page 27). 15 Program name of activated program (see page 15). Page 15 Operating Manual EM CPD300 12/18...
  • Page 19 Sets exchange cycles. 12 cycles means one chamber volume is completely exchanged. Minimum is 12 cycles. 10 Sets heating speed for critical point. Three possibilities: slow, medium, fast. 11 Sets gas out speed. Possibilities: slow, medium, fast. Page 16 Operating Manual EM CPD300 12/18...
  • Page 20 Slow speed can be decreased up to 20% of its normal speed. 12 Scrolls programs from 1-10. 13 Confirms activated program. Switch to main screen. Page 17 Operating Manual EM CPD300 12/18...
  • Page 21 Filler/Holder Panel: Filler and holder panel. Status display of fillers and holders. Sets specific holder and fillers. Combination of holders and fillers depends on their volumes. Confirms filler and holder setting. Page 18 Operating Manual EM CPD300 12/18...

  • Page 22: Programming By Example

    Programming by example Press Programs Press Program Name Type Program name Page 19 Operating Manual EM CPD300 12/18...
  • Page 23 Press OK to confirm program name Activate stirrer Select stirrer speed Speed display will disappear after 4 sec. Page 20 Operating Manual EM CPD300 12/18...
  • Page 24 Activated stirrer symbol becomes green Set CO -IN speed Press Fillers Program Filler/Holder combination Example: 1/2 Holder with 1/6 and 1/3 Filler Page 21 Operating Manual EM CPD300 12/18...
  • Page 25 Press OK to confirm Filler/Holder settings Press Delay Set Delay time Example: Set to 2min Page 22 Operating Manual EM CPD300 12/18...
  • Page 26 Press OK to confirm Press Exchange Speed Set Exchange Speed Example: set to 5 Exchange Speed display will disappear after 4 sec. Page 23 Operating Manual EM CPD300 12/18...
  • Page 27 Press Exchange Cycles Set Exchange Cycles Example: set to 12 Page 24 Operating Manual EM CPD300 12/18...
  • Page 28 Press OK to confirm Set heating speed Example: from medium to fast Set Gas out speed Example: from fast to slow Page 25 Operating Manual EM CPD300 12/18...
  • Page 29 Select slow speed setting Example: Set to 100% slow Slow Gas out Speed display will disappear after 4 sec. Press OK to confirm Page 26 Operating Manual EM CPD300 12/18...
  • Page 30 Press Start to activate program Test Page 27 Operating Manual EM CPD300 12/18...

  • Page 31: Programming The Em Cpd300 Procedure

    Programming the EM CPD300 procedure Before placing the sample into the pressure chamber, program the EM CPD300 protocol. Switch to the program screen panel and follow the instructions under Program Panel (see page 17-25). Do not forget adjustment of the pressure threshold for the bottle empty function (see page 38-39).

  • Page 32: Preset Of Cooling Temperature Of The Sample Chamber

    20 °C, the cooling temperature of the chamber should be set to 15 °C. Recommended pressure chamber -Temperature (°C) cooling temperature (°C) Fully open the shut-off valve of the CO bottle. Page 29 Operating Manual EM CPD300 12/18...

  • Page 33: Starting The Em Cpd300 Auto Procedure

    Starting the EM CPD300 auto procedure After programming go back to the main menu and press start. Time and timer function Press timer function (1). The timer display pops up (2). Page 30 Operating Manual EM CPD300 12/18...
  • Page 34 Now you can change the local time (3)… …or set the timer with the keypad (4). The timer function is activated if watch symbol is green (5). Activated time fields become blue (6). Page 31 Operating Manual EM CPD300 12/18...
  • Page 35 In the main screen the green watch symbol beside the local time indication shows that time is activated (8). By activating the timer no programming or other changes are possible. To cancel timer settings, press stop (9) … Page 32 Operating Manual EM CPD300 12/18...
  • Page 36 …then pause (10)… …and cancel (11). Touch Process time field to check (in the main screen) the timer starting time (12)… Page 33 Operating Manual EM CPD300 12/18...
  • Page 37 …and touch starting time to check finishing time (13). Page 34 Operating Manual EM CPD300 12/18...

  • Page 38: Manual Operation Of Em Cpd300

    Manual Operation of EM CPD300 Screen descriptions Initialization screen: Touch screen after initialization Main Screen before start: Dark gray buttons can be activated, light gray buttons are inactive! Page 35 Operating Manual EM CPD300 12/18...
  • Page 39 11 Sets Gas out speed. Possibilities: slow, medium, fast. Slow speed can be decreased up to 20% of its normal speed. Adjustment under Settings / advanced slow Gas Out (see page 37). 12 Sets Time (see page 27). Page 36 Operating Manual EM CPD300 12/18...

  • Page 40: Filling The Chamber With Exchange Fluid

    (15°C factory setting). The cooling temperature depends on the bottle temperature. Please see sub chapter “7.6.1 CO2-Bottle Temperature / Pressure Function” to select the appropriate cooling temperature. The cooling unit automatically keeps the temperature at the preset cooling temperature or below. Page 37 Operating Manual EM CPD300 12/18...

  • Page 41: Filling The Co Into Sample Chamber

    This procedure of filling, mixing, and draining needs to be repeated several times (minimum 12 times) before heating to remove all the exchange fluid from the specimens. Multiple and, or larger specimens require more changes. Page 38 Operating Manual EM CPD300 12/18...

  • Page 42: Heating The Sample Chamber

    If this is not possible, it is recommended to keep the specimen in a desiccator until it is processed further. 10. Switching off the Unit Leave the sample chamber open. Leave the CO bottle closed. Switch off the main switch located on the unit. Page 39 Operating Manual EM CPD300 12/18...

  • Page 43: Setting Panel

    In the auto version the advanced slow Gas-out speed adjustment is accessible under program display (see page 15). System counts every complete run for maintenance advices (see page 40). Volume change. 10 Physical unit change. Page 40 Operating Manual EM CPD300 12/18...

  • Page 44: Co -Bottle Temperature / Pressure Function

    Example: If the bottle surface temperature is 22 °C the estimated CO temperature is 20 °C, the cooling temperature of the chamber should be set to 15 °C. Recommended pressure chamber -Temperature (°C) cooling temperature (°C) Page 41 Operating Manual EM CPD300 12/18...

  • Page 45: Adjustments Of Pressure Threshold For Bottle Empty Function

    The threshold for this function has to be adapted to the CO temperature. See list below. Green marked values indicate optimal working temperature and pressure range. Recommended Pressure of full CO -Temperature (°C) threshold for pressure Bottle (bar) (bar) Page 42 Operating Manual EM CPD300 12/18...
  • Page 46 45. The CO temperature can be estimated by measuring bottle surface with thermometer. CO temperature is then about 1-2 °C cooler then the bottle surface. Press „ Update “ to confirm. Page 43 Operating Manual EM CPD300 12/18...

  • Page 47: Software Update

    14. Software Update Insert USB stick with EM CPD300 software. Software modules will be automatically recognized by the system. Choose the module you want to update. Page 44 Operating Manual EM CPD300 12/18...
  • Page 48 After software update, press back. Page 45 Operating Manual EM CPD300 12/18...

  • Page 49: Service Notes

    15. Service Notes 15.1 Gas-Out filter maintenance After 100 runs are performed, the following note appears: Under Chapter 14 (Maintenance) the poral filter replacement is described. Page 46 Operating Manual EM CPD300 12/18...

  • Page 50: General Maintenance

    15.2 General maintenance After 500 runs a general maintenance is recommended! Page 47 Operating Manual EM CPD300 12/18...

  • Page 51: User Maintenance

    EM CPD300 has to be disconnected. 16.1 Poral filter replacement Remove front cover Use a 17 size wrench to open the nut in the valve block. Remove nut, which includes porous filter, from the valve block. Page 48 Operating Manual EM CPD300 12/18...
  • Page 52 Remove poral filter with spring from the nut. Page 49 Operating Manual EM CPD300 12/18...

  • Page 53: Cleaning

    Poral filter (75 - 100 µm) for CO Outlet (right). Reassemble poral filter in to the nut and screw nut in valve block. 16.2 Cleaning All surfaces can be cleaned with aqueous reagents or 60% ethanol and a clean cloth. Page 50 Operating Manual EM CPD300 12/18...

  • Page 54: Warnings And Troubleshooting

    17. Warnings and Troubleshooting The list below summarizes Warnings (W...). Regarding most of the displayed information, just follow the instructions; in the case of error messages, contacting your local Leica Service is required. Code Warning Cause Action Com connection 10E11...
  • Page 55 (RoHS directive) 2011/65/EU (RoHS Richtlinie) Vienna/Wien/Vienna, 21/07/2017 Dr. Paul Wurzinger Entwicklungsleiter/R&D Manager/Chef du service développment Leica Mikrosysteme GmbH · Hernalser Hauptstrasse 219 · A-1170 Wien T +43 1 48899 0 · F +43 1 48899 350 CONNECT www.leica-microsystems.com WITH US!
  • Page 56 CONNECT WITH US! Leica Mikrosysteme GmbH | Vienna, Austria T +43 1 486 8050-0 | F +43 1 486 8050-30 www.leica-microsystems.com...

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