Related Manuals for Leica PAULA
Summary of Contents for Leica PAULA
- Page 1 From Eye to Insight PAULA Personal AUtomated Lab Assistant Instructions for use Leica Microsystems CMS GmbH Instructions for use 11934164, Revision 1.0, 2018-06-15 •...
- Page 2 Coyprights Copyrights All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustra- tions (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH.
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Page 3: Table Of Contents
Contents Contents Important Notes about this Manual ....4 1.1 Text symbols and their meanings ......4 Intended Use............6 Safety notes ............8 3.1 General safety notes ..........8 3.2 Electrical Safety ............. 9 3.3 Ambient conditions ..........10 3.4 Transport and storage ......... -
Page 4: Important Notes About This Manual
1. Important notes about thIs manual 1. Important Notes about this Manual Caution! These Instructions for Use are an essential component of the product; they must be read carefully before the product is assembled, put into operation or used, and must be kept for later reference. These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument. - Page 5 1. Important notes about thIs manual Warning of hazardous electrical voltage! Risk of electrical shock! Warning of electromagnetic field Warning of hazardous laser radiation – take appro- priate safety precautions! Instructions for disposing of the instrument, its ac- cessories and consumables. Explanatory note Item not contained in all equipment configurations Manufacturing date, e.g.
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Page 6: Intended Use
Reasonably foreseeable misuse The following are prohibited: Using PAULA system for any purpose not in accordance with the Declaration of Conformity (e.g. using it as an in-vitro diagnostic product according to Council Directive 98/79/EC or as a medical product according to... - Page 7 (such as to the camera ports), since this radiation poses a hazard for the user (eye injury in particular). For use of the microscope with lasers, Leica Microsystems CMS GmbH offers special microscope variants with additional safety features. Laser couplings require corresponding safety devices that have to be inspect- ed and installed by trained personnel.
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Page 8: Safety Notes
It is not allowed to put the power supplies, PC, or monitor/tablet into the incubator. Only PAULA is appropriate for this application. -
Page 9: Electrical Safety
Use the original power supply only. Other power supplies must not be used. If the original power supply fails or is damaged, it must be replaced. Repair is not permitted. Original power supplies are available from your Leica branch office or Leica dealer. -
Page 10: Ambient Conditions
This instrument has been developed and tested in accordance with CISPR 11 Class B Caution! Fully equipped, PAULA ( digital imager without NUC PC) weighs more than 6.5 kg. For placing the imager inside the incubator the user has to make sure that the shelves are capable of holding this weight. -
Page 11: Transport And Storage
Dust and loose dirt particles can be removed with a soft brush or lint-free cotton cloth. 3.6 Disposal After the end of the product’s life, please contact Leica Service or Leica Sales on how to dispose of it. Please observe the national laws and ordinances which, for example, implement and ensure compliance with EU directive WEEE. -
Page 12: System Overview
Control of digital imager including image acquisition, t-lapse, and confluence check, browse and search function Optional apps* Transfection efficiency Cell Counting *Please check the Leica app store for recently released additional apps. 4.1 Digital Imager Fig. 1 Overview PAULA Scan opening Bar code reader... -
Page 13: Intel Nuc Pc
4. SyStem Overview Fig. 2 Dimensions of the digital imager (in mm): Fig. 3 Top View PAULA 4.2 Intel NUC PC Please refer to the instruction for use delivered with the Intel NUC PC. -
Page 14: Notes For The User
5. Notes for the user 5. Notes for the user The PAULA system is an easy to use and easy to maintain digital device. However we recommend to read the following notes in order to obtain a long life time span and best results. -
Page 15: Local Area Network Considerations
You can control PAULA either directly via a connected touch-monitor (no local area network LAN needed) or you can operate PAULA using tablets (Windows, Android or iOS tablets are supported). For this purpose PAULA (NUC PC) must be integrated into your local network. -
Page 16: Supported Control Devices
WiFi network. If you want to use a tablet to control PAULA this client needs to be in the same network as the Intel NUC PC. Use the set-up service of your tablet to connect the client (Windows, Android , or iOS tablet ) to the desired network. - Page 17 7. Supported control deviceS 7. Supported control devices You can control PAULA and the NUC-PC with a touch monitor, or an iOS, Android or Windows tablet. The minimum requirements for tablets are: Dimensions Android Windows iOS 10 and above Android Version 7 and above...
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Page 18: Getting Started
Some of our packing material has symbols indicating environment-friendly recycling. Before you start the set-up of PAULA please remove the transport lock at the rear side of the digital imager. 8.3 Placement of the Digital Imager The Digital Imager can be placed on a laboratory bench. -
Page 19: Connectivity Set-Up
Plug-in the power supply cord at the rear side of the NUC PC and connect the power supply to the mains Switch on NUC PC using the on/off switch at the front The status LED on the front side of the PAULA device gets green The device can be controlled either by touch monitor, iPAD, Android tablet, or Windows tablet. - Page 20 Please follow instructions for use of your touch monitor. Switch on NUC PC and the touch monitor An icon of PAULA App is displayed on the screen. Start App by double clicking on the icon Click on “PAULA NUC PC”...
- Page 21 Make sure that the LAN meets the minimum requirements for PAULA (-> 6.1). Turn on NUC PC Turn on your tablet, switch to App store and search for Leica Microsystems and download the PAULA App from the app store. PAULA app icon will be visible on your screen.
- Page 22 PAULA app on your tablet directly (-> 8.6). If the NUC PC does not have a permanent IP address please click on the Leica icon on the task bar of your desktop of your touch monitor. This starts the PAULA service.
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Page 23: Software Overview
Name of your PAULA The name of the connected PAULA is displayed The number reflects the last 3 digital of the serial number of your device If more than one PAULA is connected a pull-down list of all PAULA is provided... - Page 24 Set-up: field of view, help, user management, sign-in Field of view: you can switch between a large FOV and a higher resolution image of your specimen. If more than one PAULA is attached: Define display of the images Help tutorial with the most important steps User management, further configurations, and available optional apps (->...
- Page 25 (e.g. the outline of the confluence check on a particular image) If you use the auto-naming system of PAULA the following abbreviations used in the names help you to identify the type of data:...
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Page 26: Log-In
For security reasons, we recommend creating a new administrator account and deleting the default account. User accounts without administrator privileges can also be created (-> 9.2). Note: You can start PAULA app without logging in. But as “Guest” you have only limited access to features of PAULA. -
Page 27: User Management
If the logged-in user does not touch the system for 30 minutes the user is logged-out automatically and an- other user can log-in. During t-lapse no other user ( except administrator) can log-in. The system is locked for other users as long as the t-lapse is executed. If a guest is using PAULA other users can log in. -
Page 28: Create And Modify Accounts
9. User management 9.2 Create and modify accounts Switch to User Management Go to Users Create new user, assign administrator rights and delete current user Note: If you want to benefit from the notification function you need to provide your e-mail address and enable the notification service in your user account. -
Page 29: Workflow
The naming of the flask is based on the information provided above. You can always overwrite the default nam- ing of the image. The auto-naming system provided by PAULA allows easy traceability of results and convenient search for re- sults. Component of the auto-naming are following abbreviations. This makes it easy to identify the type of data in the Data View. -
Page 30: T-Lapse
10. WorkfloW 10.1.1 Using the barcode reader Barcodes on the flask/petri dish minimize identification mistakes and spelling errors. Flasks with printed bar- code are commercially available. Alternatively you can stick manually a barcode on the side of the flask. 10.2 T-lapse 10.2.1 Define t-lapse Open t-lapse control panel in the workflow bar... - Page 31 10. WorkfloW 10.2.2 Summary of t-lapse After acquisition of a t-lapse switch to Data View Activate flask list Double click on the flask of interest and select t-lapse of interest. The summary page of the t-lapse is displayed In case a cell based assay has been performed dur- ing acquisition of the t-lapse you can select the re- sult of the assay over time in the pull-down list You can switch to a list view of the individual images...
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Page 32: Cell Based Assays
Coverage of a specified scratched area. Not yet available Note: Not all of the above mentioned apps are currently available. You will be informed by PAULA if new apps are available. If you are not connected to the internet please ask your local sales representative. - Page 33 Use the slider to perform a fine tuning of the detec- tion. For final result press ”Validate and Save”. PAULA switches automatically to a large field of view if not activated yet to perform the confluence check. The image outline and result of the check are stored.
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Page 34: Data Management
10.3.2 Confluence Check over time PAULA allows you to perform cell based assays over time. The results are displayed as graphs and as table. Place PAULA in the incubator and allow to acclimate for at least one hour Place specimen on the stage and focus Define the t-lapse conditions (->10.2.1). -
Page 35: Licensing Of Optional Apps For Cell-Based Assays
11. Licensing of optionaL apps for ceLL-based assays Select Image Archive (archive as zip file), and define destination on NUC PC ( my window PC) or external drive (e.g. USB stick, external hard drive). The drive letter is displayed. Finally activate “ Export”. 11. -
Page 36: Trouble Shooting
There might be a temporarily high traffic on your local LAN.( many users) Your local LAN features only 2.4 GHz (not 5 GHz as recommended) For higher stability we recommend to use a permanent IP address for PAULA NUC PC ( please ask your local IT department for support) I cannot define when to notify me about the confluence status of my cells Please switch to user management and enable “notify me”... -
Page 37: Abbreviation And Glossar
13. AbbreviAtion And glossAr 13. Abbreviation and glossar Access point (AP) Device which allows wireless connection to a wired network. Build in routers Ethernet One of the most popular wired standards to connect digital devices to a network. Ethernet port Allows a device to connect to a network via Ethernet cable. -
Page 38: Care
14. Care 14. Care Caution! Unplug the power supply before performing cleaning and maintenance work! Protect electrical components from moisture! Microscopes in warm and warm-damp climatic zones require special care in order to prevent the build up of fungus. The microscope should be cleaned after each use, and the microscope optics should be kept painstakingly clean. -
Page 39: Ec Declaration Of Conformity
15. EC DEClaration of Conformity 15. EC Declaration of Conformity Hereby, Leica Microsystems CMS GmbH declares that the radio equipment type Paula is in compliance with Directive 2014/53/EU. The full text of the EU declaration of conformity is available at the following internet ad- dress: http://www.leica-microsystems.com/products/light-microscopes/life-science-research/inverted-microscopes... - Page 40 Leica Microsystems CMS GmbH | Ernst-Leitz-Strasse 17–37 | D-35578 Wetzlar (Germany) Tel. +49 (0) 6441 29-0 | F +49 (0) 6441 29-2599 www.leica-microsystems.com...