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6 Static Needle Holder Installation and Use
With the upgraded HPLC-Chip Interface, a second top plate has been included to provide users
an alternative to the HPLC Chip Cube Interface so that "classic" Nanospray emitters can be
used. This top plate includes a universal needle holder, top plate, and optimized grounding
path for the needle holder.
The needle holder is designed to accommodate the standard 5 cm fixed length Nanospray
emitters. Any needles used with this holder should have tip sizes of 5 to 10 µm. Smaller tips
can be used however the user must lower the capillary voltage significantly relative to the
voltages used with the HPLC Chips. The only adjustment users should have to make is in one
axis (up or down). The best position vertically must be finalized by the user. No other position
adjustments are required by the user.
The static needle holder can be used with integrated nano columns/sprayers, static needles
(no HPLC flow), and commercially available Nanospray emitters (New Objective, Proxeon
(ThermoFisher)).
Use of Proxeon (Thermo Fisher) Nanospray Emitters
This section is still under development.
Page 32 of 33
18 July 2011
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