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6. Once the spacing is set correctly, tighten the set screw that secures the Holey Counter
Electrode in place. Once the set screw has been tightened, recheck the spacing to ensure
that there is no extra space. Otherwise, the high voltage field will not be correct.
Realign the Illumination LED and Camera
Because the Holey Counter electrode has different depth dimensions, the illumination LED
will not be pointing at the right location. This will result in poor illumination of the spray
from the chip. Therefore, the illumination LED position will need to be adjusted. This
procedure must be performed with the HPLC Chip Cube Interface not installed on the
LC/MS instrument. If this adjustment procedure is performed while the interface is
mounted on the instrument, the interface can be pushed off of the instrument by the I-
button reader. This results in the interface dropping on the floor which will damage the
interface.
1. Open the spray chamber and place on a flat surface.
2. Locate the set (grub) screw that secures the illumination LED assembly in place.
3. Using the supplied 0.9 mm Allen wrench (part number 8710-2624), loosen the set screw
slightly.
4. Use the spacing tool between the Holey counter electrode and the spray shield to make
visualizing the LED easier.
5. Rotate the LED assembly to where 1/3 of the beam is striking the edge of the Holey
Counter Electrode. See the figure below.
Page 18 of 33
This is a simulated
illumination spot showing
the desired alignment.
18 July 2011
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