Introduction To The Kingfisher Flex; Intended Use; Principle Of Operation; Consumables - Thermo Scientific KingFisher Flex Brief User Manual

Introduction to the KingFisher Flex

This Brief User Manual is for the KingFisher Flex instrument. It aims to give you a short introduction
on installing the instrument and Thermo Scientific™ BindIt™ Software as well as starting a
purification protocol from the internal software.
The complete user manuals for the KingFisher Flex instrument and BindIt Software are provided on
CD, or USB, or are available to download from www.thermofisher.com. Read the user manuals in its
entirety before operating the instrument.

Intended use

The KingFisher Flex magnetic particle processor (Figure 1) is intended for laboratory use by trained
personnel. The instrument is intended for automated transfer and processing of magnetic particles
in a microplate scale. Use for self-testing is excluded. It is recommended that Good Laboratory
Practices (GLP) is followed to guarantee reliable analyses.

Principle of operation

The KingFisher Flex magnetic particle processor (Figure 1) is designed for automated transfer and
processing of magnetic particles in microplate format.
The patented technology of the KingFisher Flex system is based on the use of magnetic rods
covered with a disposable, specially designed tip comb and plates. The instrument functions without
any dispensing or aspiration parts or devices.
Before the run samples and reagents, including magnetic particles, are dispensed manually into the
plates according to the corresponding instructions. The protocol that is selected by the user via the
keyboard and display has already been preloaded into the onboard software. BindIt Software can be
used to create and run protocols.
Transparent lid
Sliding door (open)
Figure 1. KingFisher Flex magnetic particle processor

Consumables

For details and ordering information on plastic consumables, such as plates, tip combs, etc. used with
the KingFisher Flex instrument, refer to "List of accessories and consumables" in the KingFisher Flex
User Manual (Cat. No. N07669).

Reagents

There is a wide selection of optimized Thermo Scientific™ KingFisher™ Pure Kits available for
purification of DNA or RNA. A large variety of starting materials can be used, such as blood, cells,
tissues or cell-free body fluids. The DNA or RNA purified with the KingFisher Pure Kits is of high
quality and free of proteins, nucleases, and other contaminants or inhibitors. For more details, visit
our website at http://www.thermoscientific.com/kingfisher.
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