Cleaning The Instrument Casing; Flushing The Lda - Thermo Scientific Accela 600 Hardware Manual

5
Maintenance

Cleaning the Instrument Casing

Cleaning the Instrument Casing

Flushing the LDA

104 Accela 600 and 1250 Pump Hardware Manual
If the pump enclosure becomes dirty, clean it with a soft cloth lightly moistened with a mild
detergent solution. Do not use any type of abrasive pad, scouring powder, or solvent.
To maintain optimal performance of the LDA, do the following:
• If you use buffered mobile phases, flush the pump's LDA daily with a non-buffered
mobile phase. Flushing the LDA helps prevent particulates from sticking in the check
valves, which might cause pressure problems.
• If you use mobile phases that contain acetonitrile, flush the LDA monthly with
HPLC-grade methanol or IPA. This removes the aliphatic amine residue that can build
up on the sapphire check valve seats and cause the check valves to stick.
CAUTION Wear safety gloves when handling solvents.
 To flush a buffered aqueous mobile phase out of the LDA
1. Set up the system to pump LC/MS-grade deionized water through the LDA for
10 minutes.
2. Pump LC/MS-grade methanol or IPA through the LDA for 10 minutes.
3. Pump LC/MS-grade deionized water through the LDA for an additional 10 minutes.
 To flush buffered acetonitrile mobile phases out of the LDA
1. Set up the system to pump a non-buffered solvent that is miscible with the buffered
mobile phase through the LDA for 10 minutes.
2. Pump LC/MS-grade methanol or IPA through the LDA for an additional 10 minutes.
 To flush non-buffered acetonitrile mobile phases out of the LDA
Pump LC/MS-grade methanol or IPA through the LDA for10 minutes.
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