Loop Injection With Manual Valve - Gilson PLC 2050 User Manual

Loop Injection with Manual Valve

use the six-way manual loop injection valve to manually load the sample into the sample loop
and manually inject it into the column. this action can be done at any time while the pump is
running.
Use the needle port plug attached to the handle assembly to shield yourself from possible
CAUTION
mobile phase spray from the needle port when the valve is turned from the INJECT to LOAD
position.
Rinse the valve with water after using buffer solutions to prevent crystals from forming,
NOTICE
which can cause scratches on the rotor seal of the valve.
the sample loop should be loaded when the system is stopped or during an equilibration
phase.
to inject the sample into the column:
1. rotate the manual injection valve handle to the LoaD position.
2. fit the large-bore syringe needle (supplied) on a syringe and fill it with the desired volume
of sample.
3. Pull the locking pin to unlock and remove the needle port plug.
Locking Pin
needle
Port
Figure 53
Manual Injection Valve - Needle Port
PLC 2050/2250/2500 PurifiCation SyStemS
needle
Port
Plug
| uSer'S GuiDe
55