Description - Gilson PLC 2050 User Manual

Description

the low temperature, evaporative light-scattering (eLS) detector integrated in the
PLC Purification System detects compounds in the eluent of the liquid purification
chromatography system. it is capable of monitoring eluent flow rates from 100 μL/min. to
5 mL/min.
eLS detection is a nearly universal technique that can detect any analyte less volatile
than the mobile phase. unlike other modes of detection, such as uV, it is not dependent
on the absorption of radiation, nor do the absorption characteristics of the solvent affect
detection. thus, solvents which absorb uV radiation can be used. as the solvent is completely
evaporated, a gradient can be performed to optimize the separation.
eLSD is a three step process:
1. nebulization is achieved with a venture nebulizer.
2. evaporation is achieved with a heated tube. this is an adjustable parameter.
3. Light diffusion detection is measured with a photodiode.
Liquid
nebulizer
inlet
Gas
inlet
nebulization
Chamber
Figure 1
ELSD Components
Large
Black
nut
Small
Black
nut
Siphon
Shield
Figure 3
Shield Installed over ELSD Components
PLC 2050/2250/2500 PurifiCation SyStemS PLC 2050/2250/2500 PurifiCation SyStemS
Shield
holder
nebulizing
inclined
Gas tubing
Support
Figure 2
eLSD installed on PLC
nebulization
Chamber
Shield
holder
Drain tube
| uSer'S GuiDe | uSer'S GuiDe
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