Loop Injection With Automatic Valve - Gilson PLC 2050 User Manual

Loop Injection with Automatic Valve

use the six-way automatic loop injection valve to manually load the sample in the sample
loop and inject it automatically in the column. refer to
on page 93 when using the GX-241 Liquid Handler as an autosampler.
the sample loop should be loaded when the system is stopped or during an equilibration
phase. in both cases, the red LeD Load light will be activated.
Figure 51
Automatic Load and Injection Diagrams
to inject the sample in the column:
1. fill a syringe with the desired volume of sample.
2. Place the syringe in the Luer female fitting in the top position of the injection valve
(Port 1).
3. Load the sample into the sample loop. any surplus will flow through the waste tubing
(Port 2).
● When the injection mode Valve Injection is selected in the method edition (refer to the
Gilson Glider Prep Software (GGP) User's Guide), the sample is automatically injected
before the elution phase starts, as indicated by the green LeD Inj. light. the sample is
pushed into the fluid path and is pushed into the chromatography column by the
solvents used.
54 |
oPeration PLC 2050/2250/2500 PurifiCation SyStemS
Figure 52
Injection Mode Setting
pLC with autosampler Configuration