Setting The Volume - BRAND HandyStep S Operating Manual

The possible dispensing volumes of the HandyStep
These are found beneath the transparent clip on the backside of the
instrument. There are 9 half-integral stroke setting positions, from 1 to 5.
1.
Look for the desired dispensing volume (Volume) in the table.
2.
Use the stroke setting wheel to set to the proper stroke number
(Setting) for the volume that corresponds to the PD-Tip (Tip size).
3.
Dispensing steps: depending on the PD-Tip capacity (ml), the same
desired dispensing volume per step can be delivered a different
number of times (see example below). The smaller the number of
steps, the higher the accuracy of the dispensed volume.
Example:
0.1 0.5
2 10
1
1.5 3 15
2 4 20
2.5 5 25
3 6 30
3.5 7 35
4 8 40
4.5 9 45
5 10 50
Important!
Desired dispensing volume per step: 200 µl
PD-Tip sizes to use:
2.5 ml = stroke setting 4 = max. 11 dispensing steps
5 ml = stroke setting 2 = max. 24 dispensing steps
10 ml = stroke setting 1 = max. 49 dispensing steps
Tip size (ml)
1 1.25 2.5
20 25
30 37.5
40 50 100
50 62.5 125
60 75 150
70 87.5 175
80 100 200
90 112.5 225
100 125 250
Volume (µl)
The volumes listed in the table are the only options avail-
able. Since the first dispensing step must be discarded,
the number given in the table is always 1 step less.

Setting the Volume

5 10 12.5
50 100 200
75 150 300
200 400
250 500
300 600
350 700
400 800 1000 2000 4000
450 900 1125 2250 4500
500 1000 1250 2500 5000
S are listed in tables.
®
25 50
250 500 1000
375 750 1500
500 1000 2000
625 1250 2500
750 1500 3000
875 1750 3500
49
32
24
19
15
13
11
10
9
29