Using The Transilluminator - Endress+Hauser Analytik Jena UVP Transilluminator Instruction Manual

UVP Transilluminators
If you are not using the transilluminator with an imaging system darkroom, do not operate the unit
without securing the cover. If the cover is missing, a UV Blocking Faceshield must be worn to avoid
UV exposure to the skin. UV Blocking Eyewear should be worn even with the cover in place to avoid
accidental UV exposure.

Using the Transilluminator

Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to protect the
filter surface from cuts and scratches. It is recommended that gloves be worn to avoid contact with gel
and staining agents.
Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter. If
using the transilluminator with an imaging system, the system's main power is required to be in the ON
position.
When using a transilluminator with multiple UV wavelengths, dial the knob to the appropriate
wavelength setting.
When using the Variable Intensity models, use the variable intensity settings as follows:

High: allows for UV excitation of fluorophores on gels for routine photography and for
excitation of gels with low sample concentration

Medium: Excellent for viewing and quick single-band excision

Low: Allows for positioning and preparation of gels, excising multiple bands and focusing for
photography
The UV Blocking Cover, optionally installed during setup, is adjustable to varying angles to provide
easier access to the filter surface. To adjust the angle, rotate the Phillips head screws (located on the
left sides of the cover's hinges) clockwise until enough tension has been placed on the hinge to hold the
cover at the desired angle.
After viewing/photographing the sample, turn the transilluminator off.
Permanently Installing the Safety Cover
Your UVP transilluminator includes a UV-blocking safety shield which can be installed temporarily (as
described elsewhere in this manual) or permanently, if desired. The following instructions will explain
the process of permanently installing the UVP-supplied UV blocking cover onto the UVP
transilluminator.
To complete this procedure, the following tools and parts will be required:
 Phillips-head screwdriver
 5/16" nut driver
 3/8" narrow walled nut driver

(4) hollow 3/8" nuts (supplied with safety cover)
CAUTION: This procedure requires a moderate level of technical competence. If you are not comfortable
working with electronics, tools and/or related components, contact Analytik Jena for assistance.
Note:
The transilluminator images shown in this procedure may differ in appearance from your
transilluminator. However, the procedure is the same.
(Optional)
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