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Summary of Contents for Endress+Hauser analytikjena qTOWER3 84
- Page 1 Operating Manual qTOWER³ 84 / qTOWER³ 84 G Real-Time PCR Thermal Cycler...
- Page 2 Manufacturer Analytik Jena GmbH+Co. KG Konrad-Zuse-Straße 1 07745 Jena / Germany Phone: +49 3641 77 70 Fax: +49 3641 77 9279 Email: info@analytik-jena.com Technical Service Biometra GmbH Rudolf-Wissell-Straße 30 37079 Göttingen / Germany Phone: +49 551 50 68 60 Fax: + 49 551 50 68 666 Email: service@analytik-jena.com For a proper and safe use of this product follow the instructions.
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Page 3: Table Of Contents
qTOWER³ 84 / qTOWER³ 84 G Table of contents Table of contents 1 Notes on this operating manual........................... 2 Intended use ................................. 3 Safety instructions ..............................Safety markings ..............................Requirements for the operating personnel..................... Safety instructions – transport and installation....................Safety instructions – operation........................3.4.1 General safety instructions for operation......................3.4.2 Safety instructions –... - Page 4 Table of contents qTOWER³ 84 / qTOWER³ 84 G 9.1.3 Moving the device in the laboratory........................ 32 Storage ................................33 10 Disposal ................................34 11 Specifications................................ 35 11.1 Technical data..............................35 11.2 Ambient conditions ............................37 11.3 Standards and directives ..........................37...
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Page 5: Notes On This Operating Manual
qTOWER³ 84 / qTOWER³ 84 G Notes on this operating manual Notes on this operating manual This operating manual describes the following thermocycler models: ¡ qTOWER³ 84 qTOWER³ 84 G ¡ In the text below, these devices are collectively called qTOWER³ 84, or simply ' d evice' . Differences between the models are explained in the corresponding section. -
Page 6: Intended Use
Intended use qTOWER³ 84 / qTOWER³ 84 G Intended use NOTICE The device is intended for general laboratory use. The device may only be used for the applications described in these operating instruc- tions. The manufacturer does not accept liability for any other use. The device is a thermocycler which is licensed for real-time PCR experiments that am- plify DNA via polymerase chain reaction (PCR) while using fluorescence spectroscopy for the highly sensitive detection of target sequences. - Page 7 qTOWER³ 84 / qTOWER³ 84 G Intended use LOGAN, Julie; EDWARDS, Kristin; SAUNDERS, Nick (ed.): Real-Time PCR – Current Tech- nology and Application. Norfolk UK: Caister Academic Press, 2009...
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Page 8: Safety Instructions
Safety instructions qTOWER³ 84 / qTOWER³ 84 G Safety instructions For your own safety and to ensure error-free and safe operation of the device, please read this chapter carefully before commissioning. Observe all safety instructions listed in this user manual and all messages and displayed by the control and analysis software on the monitor. -
Page 9: Safety Instructions - Transport And Installation
qTOWER³ 84 / qTOWER³ 84 G Safety instructions When using the device, observe the appropriate laboratory diligence and cleanliness ¡ to prevent contamination of the device. This reduces the risk of the user being con- taminated with potentially infectious material and the risk of cross-contamination of the samples. -
Page 10: Safety Instructions - Protection Against Explosion And Fire
Safety instructions qTOWER³ 84 / qTOWER³ 84 G Do not touch hot sample tubes or plates and do not open them or boiling liquid may ¡ escape! 3.4.2 Safety instructions – protection against explosion and fire The device must not be operated with flammable, explosive or volatile substances. ¡... -
Page 11: Safety Instructions - Maintenance And Repair
qTOWER³ 84 / qTOWER³ 84 G Safety instructions Safety instructions – maintenance and repair Maintenance of the device is always carried out by the Analytik Jena customer service department or specialist personnel trained and authorized by Analytik Jena. Unautho- rized maintenance can damage the device. The operator may only carry out the tasks listed in the chapter "Maintenance and care". -
Page 12: Design And Function
Design and function qTOWER³ 84 / qTOWER³ 84 G Design and function Design, connections and control elements The device combines a PCR thermocycler with a patented fluorescence photometer. The lock with handle and the LED for status display are located on the front side of the device. -
Page 13: Fig. 3 Back
qTOWER³ 84 / qTOWER³ 84 G Design and function The mains connection and the power switch are located on the device rear. The interface for connection to the PC is also located on the device rear. Fig. 3 Back 1 Ventilation for fluorescence spectrome- 2 Ventilation grille on the thermal cycler 3 Service connection 4 Power connection 5 Fuse holder for device fuses... -
Page 14: Function
Design and function qTOWER³ 84 / qTOWER³ 84 G Fig. 5 Mains and connection cable Only use the mains cable supplied or a mains cable with the same specifications. Function 4.2.1 Fluorescence spectrometer The detector unit for a thermal block with 384 wells is a patented 16-channel epi-fluo- rescence photometer with fiber multiplexer and a mechanical scanning device. -
Page 15: Fig. 7 Schematic Representation Of The Light Beam Path Through A Filter Color Module
qTOWER³ 84 / qTOWER³ 84 G Design and function Measuring head The fluorescent dyes are specifically excited in the reaction mix thanks to the light and emit light of a longer wavelength. Using the lenses in the shuttle system, the emitted light is bundled and passed back to the color modules via the optical fibers. Photomultiplier (PMT) In the color modules, the light passes the beam divider followed by two emission filters and is then transferred to the photomultiplier (CPM) for detection. -
Page 16: Pcr Thermocycler
Design and function qTOWER³ 84 / qTOWER³ 84 G 4.2.2 PCR thermocycler The thermal block with 384 wells is made of aluminum that conducts heat very well. This achieves a high temperature homogeneity and uniformity in combination with heating rates of up to 4 °C/s and cooling rates of up to 2 °C/s . This powerful thermal block is suitable for high-throughput applications in particular. -
Page 17: Type Plate
qTOWER³ 84 / qTOWER³ 84 G Design and function Type plate The type plate is located on the rear of the device. It provides the following information shown in the figure: Manufacturer and address ¡ Protection class of the housing ¡ ¡ Safety symbols (Caution: Observe the accompanying documents! ¡... -
Page 18: Installation
Installation qTOWER³ 84 / qTOWER³ 84 G Installation Installation location requirements Ambient conditions The climate conditions for the installation location are listed in the technical specifica- tions (→ "Ambient conditions" 37). If required, ensure that the room is tempera- ture-controlled. Installation location require- This laboratory device is designed for indoor use. ¡... -
Page 19: Installation
qTOWER³ 84 / qTOWER³ 84 G Installation Electrical connection require- Operating voltage 100 / 115 / 230 V (AC) ments Line frequency 50 to 60 Hz Power consumption ≤ 850 W Device fuse 2 x 10 AT / 250 V Installation WARNING Risk of electric shock! Check that the mains connection conditions match those indicated on the type plate on the rear of the device. Before connecting the device to the mains network set the correct operating voltage at the voltage switch on the underside of the device. -
Page 20: Fig. 8 Voltage Selection Switch On The Bottom Of The Device
Installation qTOWER³ 84 / qTOWER³ 84 G Fig. 8 Voltage selection switch on the bottom of the device } Open the lid of the device. To do so, press in the red handle on the front until the lock clicks open. Fold back the upper part of the device. } Remove the transport lock and put it aside for later transports. -
Page 21: Operation
qTOWER³ 84 / qTOWER³ 84 G Operation Operation Switching the device on and off Switching on the device } Switch on the device via the mains switch on the rear of the device. ü The device is initialized. During this time, the status LED on the front side of the device flashes. -
Page 22: Fig. 9 Position A1 On The Sample Block
Operation qTOWER³ 84 / qTOWER³ 84 G WARNING Risk of eye injury! The rapid heating of the thermal block can result in the explosive vaporization of liquids when the thermal block is opened during the PCR run. Always wear safety goggles dur- ing operation. CAUTION Hot surfaces The thermal block, the samples, and the heated lid reach high temperatures. -
Page 23: Error Messages
qTOWER³ 84 / qTOWER³ 84 G Error messages Error messages The following chapter describes possible errors of the device. The error correction mea- sures that can be performed by the customer are limited to those listed in the following section. NOTICE If you cannot eliminate errors yourself, please contact customer service. If an error occurs, the software outputs error codes assigned to the following errors: Error code Cause... -
Page 24: Maintenance And Care
Maintenance and care qTOWER³ 84 / qTOWER³ 84 G Maintenance and care WARNING Risk of electric shock! Do not touch! Prior to commencing any maintenance or cleaning work, switch off the device and un- plug the power plug. The following maintenance and care tasks can be performed by the customer: Cleaning and disinfection of the housing and sample block ¡... -
Page 25: Cleaning The Sample Block
qTOWER³ 84 / qTOWER³ 84 G Maintenance and care Cleaning the sample block WARNING Risk of eye injury! Wear safety goggles when blasting the sample block with compressed air. Dust or reagent residue in the sample block can cause an increase in the background sig- nal. -
Page 26: Replacing Fuses
Maintenance and care qTOWER³ 84 / qTOWER³ 84 G Only disinfection agents containing ethanol or isopropanol are approved. The use of ¡ disinfection agents with ingredients or concentrations that differ from the recom- mended disinfection agents invalidates all warranty claims regarding damage to the device or non-effectiveness. If the device must be sent back to Analytik Jena for servicing, first perform decon- ¡... -
Page 27: Installing Color Modules
qTOWER³ 84 / qTOWER³ 84 G Maintenance and care Installing color modules Depending on the delivery state of the device, or when purchasing additional color mod- ules, it may be necessary to install the color modules in the device. The top device hood must be removed for this purpose. NOTICE The lens of the flourescence spectrometer is very sensitive, and can become damaged due to improper installation. - Page 28 Maintenance and care qTOWER³ 84 / qTOWER³ 84 G The pivotable holder for the color modules is now accessible. } Note the code on the color module and the position in which the color module is to be installed. Remove the old color module or dummy ele- ment (dummy module).
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Page 29: Fig. 10 Selection Window For Color Modules. Note: With Software Version 1.2 And Higher, Only The Color Module
qTOWER³ 84 / qTOWER³ 84 G Maintenance and care } Open the lid and fasten the four outer screws on the inside of the lid. } Close the lid again. ü The color modules are installed. Registering color modules in To use the color modules correctly in the software, these must be registered in the soft- the software ware. - Page 30 Maintenance and care qTOWER³ 84 / qTOWER³ 84 G ü The color module is available in the software. Removing a color module A color module that is not required can be removed from the software. } Mark the color module in the Color modules list and click on Delete. ü...
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Page 31: Transport And Storage
qTOWER³ 84 / qTOWER³ 84 G Transport and storage Transport and storage Transport NOTICE Use suitable packaging material and transport locks! Unsuitable packaging material may cause damage to the device! Only transport the de- vice in its original packaging and with the transport lock in place! Information on proper packaging is included with the device. -
Page 32: Return
Transport and storage qTOWER³ 84 / qTOWER³ 84 G 9.1.2 Return WARNING Risk of damage to health due to improper decontamination! Perform a professional and documented decontamination of the device before returning it to Analytik Jena. The decontamination report is available from the customer service department when registering the return. -
Page 33: Storage
qTOWER³ 84 / qTOWER³ 84 G Transport and storage Storage NOTICE Environmental influences and condensate formation can destroy individual components of the device! The device must only be stored in air-conditioned rooms. The atmosphere must be low in dust and free from aggressive vapors. If the device is not installed immediately after delivery or not required for prolonged pe- riods, it should be stored in its original packaging. -
Page 34: Disposal
Disposal qTOWER³ 84 / qTOWER³ 84 G Disposal The operator of the device must dispose the waste materials that occur during mea- surements (sample materials) in accordance with the statutory and local regulations. At the end of its service life, the device and all its electronic components must be dis- posed of as electronic waste in accordance with applicable regulations. -
Page 35: Specifications
qTOWER³ 84 / qTOWER³ 84 G Specifications Specifications 11.1 Technical data General data Dimensions (height x width x depth) 59 cm x 27.5 cm x 27.5 cm, 70 cm x 27.5 cm x 50 cm when opened Mass 30 kg Noise level 45 dB Safety circuits Sensor-based monitoring of the closing ¡ and locking of the sample chamber Overtemperature protection in the ¡... - Page 36 Specifications qTOWER³ 84 / qTOWER³ 84 G Fluorescence spectrometer Measuring principle Fiber-optic shuttle system with 16-fold scan- ner and color modules for the excitation and emission filters Light source 4 long-life, high-performance LEDs (RGBW) Color modules ¡ 12 color-, FRET and protein modules ¡ 6 positions in the device Detector Highly sensitive PMT (photomultiplier tube) Color modules...
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Page 37: 11.2 Ambient Conditions
qTOWER³ 84 / qTOWER³ 84 G Specifications 11.2 Ambient conditions Operation Transport, storage Temperature range +15 °C to +35 °C -10 °C to +55 °C Max. humidity 70 % 10 % to 30 % Use desiccant! Max. permissible height 2000 m Air pressure 0.7 to 1.06 bar Work environment Only suitable for operation in rooms 11.3 Standards and directives Protection class and protection The device is protection class I. - Page 38 Table of figures qTOWER³ 84 / qTOWER³ 84 G Table of figures Fig. 1 Front view................................12 Fig. 2 Open device ................................12 Fig. 3 Back..................................13 Fig. 4 Voltage selection switch on the bottom of the device ..................13 Fig. 5 Mains and connection cable..........................14 Fig.
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