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Related Manuals for Endress+Hauser analytikjena CyBio SELMA Series
Summary of Contents for Endress+Hauser analytikjena CyBio SELMA Series
- Page 1 Operating Manual CyBio SELMA Semi-automatic pipetting station...
- Page 2 Manufacturer Analytik Jena GmbH+Co. KG Konrad-Zuse-Straße 1 07745 Jena / Germany Phone: +49 3641 77 70 Fax: +49 3641 77 9279 Email: info@analytik-jena.com Technical Service Analytik Jena GmbH+Co. KG Konrad-Zuse-Straße 1 07745 Jena / Germany Phone: +49 3641 77 9449 Fax: +49 3641 77 767776 E-mail: service.lha@analytik-jena.com For a proper and safe use of this product follow the instructions.
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Page 3: Table Of Contents
CyBio SELMA Table of contents Table of contents 1 Basic information ..............................About this user manual ............................ Intended use ..............................Standards and directives ..........................2 Safety ..................................Safety labeling on the device ........................... Danger zone..............................Requirements for the operating personnel..................... Safety instructions for operation ........................ - Page 4 Table of contents CyBio SELMA Advanced functions ............................47 5.8.1 Tip replacement..............................47 5.8.2 Device settings ..............................48 5.8.3 Idle state................................49 5.8.4 Work with semi-automatic routines........................ 49 6 Troubleshooting ..............................52 Entry of incorrect values........................... 52 Operation only with inserted tip holder ......................52 Interrupt the pipetting routine with the STOP button ...................
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Page 5: Basic Information
CyBio SELMA Basic information Basic information About this user manual Content The operating manual describes the semi-automatic pipetting station CyBio SELMA. The device is intended to be operated by qualified specialist personnel observing this user manual. The manual provides information about the design and operation of the device and pro- vides operating personnel with the necessary know-how for safe handling of the device and its components. -
Page 6: Standards And Directives
Basic information CyBio SELMA The basic functions include the absorption and dispensing of liquid from and into reser- voirs, microplates, and their individual columns. The device is suitable to be operated by one person. You can equip the device exclusively with the following tip holders: ¡... -
Page 7: Safety
CyBio SELMA Safety Safety For your own safety and to ensure error-free and safe operation of the device, please read this chapter carefully before commissioning. Observe all safety instructions listed in this manual, as well as all messages and instruc- tions displayed by the control- and analysis software on the monitor. -
Page 8: Danger Zone
Safety CyBio SELMA Warning sym- Meaning Comment Warning of a danger Warning of mechanical hazard from moving point equipment parts. Warning of crushing Warning of crushing in the area of the tip mounting Danger zone Fig. 2 Hazard area 1 STOP button 2 Movement area of the pipetting head 3 Tip mounting 4 Tray with two working positions... -
Page 9: Requirements For The Operating Personnel
CyBio SELMA Safety Immediately release the rotary knob in the case of maloperation (manual opera- ¡ tion). The rotary knob will return to its mid-range position. Turn the knob in clock- wise direction in order to restore the pipetting head to zero position. ¡... -
Page 10: Safety Instructions For Maintenance And Repair
Safety CyBio SELMA The sound condition of the protection and safety equipment must be checked regu- ¡ larly. Any defects must be corrected as soon as they occur. ¡ Protective and safety equipment must never be removed, modified or switched off during operation. -
Page 11: Chemical Resistance
CyBio SELMA Safety 2.5.1 Chemical resistance Aggressive substances may damage the device. Although the materials used are resis- tant to most of the commonly used substances, material damage from aggressive sub- stances cannot be completely excluded. ¡ Before using any aggressive substances, e.g., bases, acids, or organic solutions: Check whether the materials are resistant to this. -
Page 12: Technical Description
Technical description CyBio SELMA Technical description Device configuration The semi-automatic pipetting station CyBio SELMA is used for the quick and precise pro- cessing of microplates with 96 or 384 wells in the ANSI/SLAS standard. The device has a pipetting head with 96 or 384 plungers that work simultaneously, with which it aspi- rates and dispenses liquids via the proven tip sealing system. -
Page 13: Control Elements
CyBio SELMA Technical description The device is mainly operated via the touchscreen, which can be folded upwards. There- fore, you can work sitting or standing. Two buttons on the support foot of the device al- low you to choose between "pipetting" ("pip" button) and "pipetting with saved values" ("move + pip"... -
Page 14: Fig. 5 Direction Of Rotation And Travel
Technical description CyBio SELMA The pipetting head is not moved here, only the plungers. Push button "move + pip" You can run pipetting routines repeatedly using the "move + pip" button. In contrast to the "move + pip" button, the "pip" button also triggers the vertical move- ment of the pipetting head. -
Page 15: Tray
CyBio SELMA Technical description Menu H: -0.0mm Priming Pipetting Sample Dilution Repeat Dispensing Serial Dilution Device Settings Change Tips Fig. 6 Button layout 1 Information about the current pipetting 2 Current height of the pipetting head step in the menu 3 Working area with various control ele- ments and information Tray The tray has two working positions. -
Page 16: External Power Supply Unit
Technical description CyBio SELMA Fig. 8 Guide groove I and II External power supply unit The power supply unit is a wide range power supply unit. The power is supplied on the primary side at a power outlet with 100 to 240 V ±10 % (1.7 A max); 50/60 Hz. On the secondary side, the power supply unit provides a voltage of 24 V (2.5 A max.). -
Page 17: Device Versions
CyBio SELMA Technical description Fig. 10 Device rear 1 Type plate 2 Cover flaps or connectors 3 On/off switch (O/I) 4 Service interface 5 DC input Depending on the device version, two cover caps or a cover flap and a connector are at- tached to the rear side. -
Page 18: Scope Of Delivery
Technical description CyBio SELMA Scope of delivery The scope of delivery includes: Pipetting station with moving tray ¡ Power supply unit ¡ Country-specific power cord ¡ ¡ Box with CyBio TipTray (in standard quality) ¡ Operating instructions ¡ Optional accessories Function With the pipetting station CyBio SELMA, you can process microplates semi-automati- cally in the ANSI/SLAS standard. -
Page 19: The Pipetting Cycle
CyBio SELMA Technical description The Pipetting Cycle A pipetting cycle always consists of: Aspiration (absorption of liquid) ¡ Dispensing (dispensing of liquid) ¡ Blowout (ejection of residual volume) ¡ ¡ Return the plungers to the zero position When the plunger has returned to the zero position, the next pipetting cycle can begin. Aspiration and dispensing of liquid is accomplished by plunger motion. - Page 20 Technical description CyBio SELMA Pipetting When pipetting, the device aspirates an exact volume of liquid without an additional overstroke. The device delivers the volume with a blowout into the target cavity. Zero-position The plunger starts in the zero position above the source cavity. The pipette tip is immersed in the liquid.
- Page 21 CyBio SELMA Technical description Return to the initial position / zero position After the blowout, the plate returns the plunger to the zero position. The pipetting cycle is finished. Reverse pipetting With reverse pipetting, the device aspirates the liquid with an additional overstroke vol- ume.
- Page 22 Technical description CyBio SELMA Dispense additional volume After aspiration, a small additional volume is dispensed initially. This step ensures that the freeplay has been overcome and the subsequent step dis- penses the nominal volume correctly. Dispense nominal volume (1) The nominal volume is dispensed into the target cavity. The plate moves the plunger down, compressing the air cushion in the chan- nel.
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Page 23: 3.10 System Precision
CyBio SELMA Technical description Fig. 12 Overcoming the freeplay after aspirating 3.10 System precision The device works on the principle of air displacement. This means that the plungers, mechanically connected to a common drive, move respectively in an air space created by the pipette tips and the internal device seals. -
Page 24: Installation And Commissioning
Installation and commissioning CyBio SELMA Installation and commissioning Installation conditions 4.1.1 Ambient conditions Climatic conditions The requirements for the ambient conditions at the installation location are set out in the specifications. If required, ensure that the room is temperature-controlled. Observe the following instructions when selecting the installation site: This laboratory device is designed for inside use. -
Page 25: Commissioning
CyBio SELMA Installation and commissioning Commissioning Set up and connect the device NOTICE Damage to the electronics due to condensation Significant temperature differences can lead to the formation of condensation which can damage the device’s electronics. ¡ After long-term storage or transport in a colder environment, allow the device to ac- climatize at room temperature for at least one hour before switching it on. -
Page 26: Function Tests
Installation and commissioning CyBio SELMA } Switch on the device at the rear side of the device. Fig. 14 Device rear 1 Type plate 2 Cover flaps or connectors 3 On/off switch (O/I) 4 Service interface 5 DC input Initialize the device Each time the device is switched on, the start screen appears briefly on the touchscreen. -
Page 27: Leak Test
CyBio SELMA Installation and commissioning NOTICE! Vacuum-packed microplates should be unpacked at least 1 day before mea- surements are taken. ¡ Cover foil for microplates ¡ Shakers for microplates ¡ Centrifuge for microplates Use a microplate that fits the pipetting head. Reagents for function tests: p-nitrophenol ¡... -
Page 28: Precision Test
Installation and commissioning CyBio SELMA } After 30 minutes, move tips from the solution. } Check the liquid levels of all pipette tips. A ruler for visual connection of the level point on the foremost and rearmost pipette makes reading easier. } Document all peaks whose level has changed. -
Page 29: Accuracy Test
CyBio SELMA Installation and commissioning } Mask the microplate. } Dispense the remaining volume into the storage vessel with a blowout. } Mix or centrifuge the solutions. Apply the settings from the table below for both pro- cesses. Device 96-well microplate 384-well microplate 700 rpm orbital shaker 15 min... - Page 30 Installation and commissioning CyBio SELMA } Place a reservoir of demineralized water at working position 1 of the tray. } Pre-wet the pipette tips with the pipetting mode Priming. Settings: Volume = Maxi- mum volume, Cycles ≥ 3 } Run the accuracy test in the pipetting mode Reverse Pipetting. } Weigh an empty, capped microplate.
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Page 31: Operation
CyBio SELMA Operation Operation WARNING Warning of biohazard The device handles biological and biochemical substances that are potentially patho- genic. ¡ Wear personal protective equipment when handling these substances. ¡ Observe all instructions and specifications in the safety data sheets. Observe na- tional regulations when handling these substances. -
Page 32: Operate The Device Via Touchscreen
Operation CyBio SELMA Operate the device via touchscreen Operate the software (general) If parameters are to be entered, the touchscreen shows you the general input window. The input window shows a keypad. You can use the displayed keys to enter digits, let- ters, and special characters. -
Page 33: Priming
CyBio SELMA Operation The main menu The Menu display contains all the buttons for the basic pipetting modes. You can also use this menu to access the extended functionalities. Menu H: -0.0mm Priming Pipetting Repeat Sample Dilution Dispensing Serial Dilution Device Settings Change Tips Fig. 16... - Page 34 Operation CyBio SELMA } In the Priming Options menu, set the following parameters: Cycles and Blowout. Cycles Blowout ü The input window appears after pressing the respective key. } Enter the desired values and apply with Apply. If you press the Default button after making a change, the device automati- cally reverts to the default values for all parameters.
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Page 35: Pipetting
CyBio SELMA Operation Model CyBio SELMA CyBio SELMA CyBio SELMA CyBio SELMA 96/25 µl 96/60 µl 96/250 µl 96/1000 µl CyBio SELMA CyBio SELMA 384/25 µl 384/60 µl Values Area Stan- Area Stan- Area Stan- Area Stan- dard dard dard dard Volume 0.01 25.0 0.10 60.0 0.1 …... - Page 36 Operation CyBio SELMA } Set the absorption and dispensing speed of the liquid in the Speed menu. Confirm the value with Apply. High The following values apply for the CyBio SELMA 96/250 µl model: ¡ Slow: 40 µl/s Medium Medium: 120 µl/s ¡ Fast: 180 µl/s ¡...
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Page 37: Diluting
CyBio SELMA Operation Model CyBio SELMA CyBio SELMA CyBio SELMA CyBio SELMA 96/25 µl 96/60 µl 96/250 µl 96/1000 µl CyBio SELMA CyBio SELMA 384/25 µl 384/60 µl Values Area Stan- Area Stan- Area Stan- Area Stan- dard dard dard dard Volume 0.01 25.0 0.10 60.0 0.1 …... - Page 38 Operation CyBio SELMA } Set the absorption and dispensing speed of the liquid in the Speed menu. Confirm the value with Apply. High The following values apply for the CyBio SELMA 96/250 µl model: ¡ Slow: 40 µl/s Medium Medium: 120 µl/s ¡ Fast: 180 µl/s ¡...
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Page 39: Dispensing
CyBio SELMA Operation } Press the Pipetting button on the touchscreen or the "pip" button on the left support foot of the device. ü The pipetting process is finished. The following table shows the possible settings in the pipetting mode Sample Dilution for all device models, with a value range and default settings respectively. - Page 40 Operation CyBio SELMA } From the main menu, select pipetting mode using the corresponding key. Menu H: -0.0mm Priming Pipetting Repeat Sample Dilution Dispensing Serial Dilution Device Settings Change Tips } In the Repeat Dispensing menu, set the following parameters: Options, Dispensing volume, Dispensing steps and Speed.
- Page 41 CyBio SELMA Operation NOTICE Pipetting head can hit the upper table In order to be able to aspirate or dispense liquids at the lower working position of the tray, the travel area of the pipetting head must be free. ¡ For pipetting at the lower working position: slide the upper table all the way to the left.
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Page 42: Reverse Pipetting
Operation CyBio SELMA Relationships between the parameters: CyBio SELMA 96/25 µl Constraint Number of dispensing steps x dispensing vol- ume ≤ 24 μl Automatic calculation of the aspirated vol- Aspirated volume = number of dispensing steps x dispensing volume + 1 μl CyBio SELMA 96/60 µl Constraint Number of dispensing steps x dispensing vol- ume ≤ 58 μl... - Page 43 CyBio SELMA Operation } From the main menu, select pipetting mode using the corresponding key. Menu H: -0.0mm Priming Pipetting Repeat Sample Dilution Dispensing Serial Dilution Device Settings Change Tips } In the Reverse Pipetting menu, set the following parameters: Aspirate Options, Aspirate volume, Dispensing volume and Speed.
- Page 44 Operation CyBio SELMA – Press the Pipetting button on the touchscreen or the "pip" button on the left sup- port foot of the device. NOTICE Pipetting head can hit the upper table In order to be able to aspirate or dispense liquids at the lower working position of the tray, the travel area of the pipetting head must be free.
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Page 45: Serial Dilution
CyBio SELMA Operation Serial dilution With serial dilution, the device absorbs a defined volume column by column. Next, the device dispenses the volume absorbed again column by column into a specific volume provided. The liquids are then mixed. Preparation With serial dilution, the device uses a tip holder that is equipped with only eight tips (96 channels) or 16 tips (384 channels) in a column. - Page 46 Operation CyBio SELMA – Place the target plate on the tray. Position the gap to be filled under the pipette tips. – Set the required dispensing height of the pipetting head. – Press the Pipetting button on the touchscreen or the "pip" button on the left sup- port foot of the device.
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Page 47: Advanced Functions
CyBio SELMA Operation Model CyBio SELMA CyBio SELMA CyBio SELMA CyBio SELMA 96/25 µl 96/60 µl 96/250 µl 96/1000 µl CyBio SELMA CyBio SELMA 384/25 µl 384/60 µl Mixing 0 … 0 … 0 … 0 … 250 cycles Blowout 0.7 … 1.0 … 7.0 … 70.0 16.0 …... -
Page 48: Device Settings
Operation CyBio SELMA 5.8.2 Device settings You can adjust the basic settings of the device. The basic settings include: Function Meaning Standby Time until the activation of idle state of the device. During the idle state, the design elements flash. Vertical speed Vertical speed of the pipetting head during the function Motion &... -
Page 49: Idle State
CyBio SELMA Operation } If necessary, get the following values h ere: Plunger cycles, total volume, Z-axis cycles, and total Z-axis travel. } The display switches back to the Device Settings sub-menu with Continue. Name Unit Value area Standard Idle state 0…180... - Page 50 Operation CyBio SELMA ü The device automatically carries out the liquid handling steps previously carried out manually with the saved parameter sets and set pipetting heights. Create and save a routine Options Speed Volume Load Save Start Menu Change Tips } Select a pipetting mode.
- Page 51 CyBio SELMA Operation The saved heights are fixed. However, the liquid level in the reservoirs normally de- ¡ creases as the method progresses. If you are pipetting large volumes or repeating a routine frequently, the liquid level will decrease quickly. There is a risk that the de- vice will draw air bubbles into the pipette tip during aspiration.
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Page 52: Troubleshooting
Troubleshooting CyBio SELMA Troubleshooting The device displays malfunctions. If the malfunctions are caused by the operator, opera- tion can usually be continued after the fault has been eliminated. In the event of malfunctions, check all possible sources of error. If the malfunction per- sists even after the error has been corrected, or if problems other than those described occur, contact the customer service department of Analytik Jena or the authorized ser- vice partner. -
Page 53: Error Handling
CyBio SELMA Troubleshooting } Press the Continue button. ü The plungers return to the zero position and the device is ready for operation again. Error handling Device cannot be activated If the device cannot be switched on, check the following points: ¡... -
Page 54: Power Failure
Troubleshooting CyBio SELMA Error by vertical drive. ¡ ¡ Error on aspiration. ¡ Error on dispensing. ¡ Error on blowing out. Error on piston motion to zero-position. ¡ Error on piston reference motion. ¡ Unknown error. ¡ ¡ System error ¡... -
Page 55: Maintenance And Care
CyBio SELMA Maintenance and care Maintenance and care The operator may not undertake any service or maintenance work to this device and its components other than that specified in these instructions. Observe the information in the "Safety instructions" section for all maintenance work. Compliance with the safety instructions is a prerequisite for the error-free operation of the device. -
Page 56: Service The Plunger Sealing System
Maintenance and care CyBio SELMA Service the plunger sealing system Tip holder NOTICE Risk of plunger damage Direct contact of the tip holder with the plungers can damage the plungers. ¡ Never insert a tip holder without pipette tips into the device. A tip holder inserted with no pipette tips installed will void any warranty claims. - Page 57 CyBio SELMA Maintenance and care } Reinsert the tip holder and tighten using the menu command. Change Tips. ü The sealing mat is cleaned.
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Page 58: Transport And Storage
Transport and storage CyBio SELMA Transport and storage Transport When transporting the device, observe the safety instructions in the "Safety instructions" section. Avoid the following during transport: Impact and vibration ¡ Risk of damage due to shock, impact or vibration! ¡... -
Page 59: Moving The Device In The Laboratory
CyBio SELMA Transport and storage Fig. 18 Attach transport locks to the device } Place the transport lock around the tip holder and the tray on the front of the device as shown in the image. } Grab the device behind the pipetting head, lift it, and place it in the lower PE-mold. CAUTION! Risk of injury when lifting the device. -
Page 60: Storage
Transport and storage CyBio SELMA Fig. 19 Lift the device Storage NOTICE Risk of device damage due to environmental conditions Environmental influences and condensation can destroy individual components of the device. ¡ Only store the device in air-conditioned rooms. ¡ Ensure that the atmosphere is free of dust and corrosive vapors. If the device is not installed immediately after delivery or not required for longer periods, it should be stored in its original packaging. -
Page 61: Specifications
CyBio SELMA Specifications Specifications General characteristics Designation/type CyBio SELMA Dimensions (W x H x D) 307 x 480 x 325 mm ¡ 307 x 520 x 325 mm ¡ (for model CyBio SELMA 96/1000 µl) Mass (depending on model) 18 to 20 kg Mass with accessories and packaging 23 to 25 kg Channels 96/384 Pipetting head Motorized height adjustment (in Z-direction) Plate positions Storage capacity >10 Parameter sets for each pipetting mode... - Page 62 Specifications CyBio SELMA Model CyBio SELMA Pipetting head 96 Channel head (1000 µl) 96/1000 µl Volume area 10 μl to 1000 μl Can be selected in 0.1 µl step Precision (CV) >25 to 100 μl ≤2 %; >100 to 1000 μl ≤1 % Tip types 1000 µl Deep Well Model CyBio SELMA 384/25 µl Pipetting head 384 Channel head (25 µl) Volume range* 0.5 μl to 25 μl...
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Page 63: Disposal
CyBio SELMA Disposal Disposal The operator is responsible for correct disposal of biological samples in accordance with statutory regulations. Dispose of consumables that are contaminated with hazardous substances in accor- dance with the applicable national and international regulations on safety and envi- ronment. - Page 64 List of figures CyBio SELMA List of figures Fig. 1 Safety labeling on the device..........................Fig. 2 Hazard area ................................Fig. 3 Device configuration ............................12 Fig. 4 Device rear................................13 Fig. 5 Direction of rotation and travel........................... 14 Fig. 6 Button layout................................
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