Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for Endress+Hauser Analytik Jena Biometra TRIO Series
Summary of Contents for Endress+Hauser Analytik Jena Biometra TRIO Series
- Page 1 Operating manual BiometraTRIO Triple Powered PCR thermal cycler...
- Page 2 Biometra GmbH (Analytik Jena AG) Manufacturer Rudolf-Wissell-Str. 30 37079 Göttingen Germany Phone + 49 551 50 68 6-0 + 49 551 50 68 6-66 Email Info@analytik-jena.com Biometra GmbH Service Rudolf-Wissell-Str. 30 37079 Göttingen Germany Phone + 49 551 50 68 6 -12, -14 or -16 + 49 551 50 68 6-66 Email Service@analytik-jena.com...
-
Page 3: Table Of Contents
Biometra TRIO Manual Contents Contents Basic Information ......................9 User manual notes ...................... 9 Intended use ......................10 Warranty and liability....................10 Safety instructions ......................11 General notes ......................11 Safety markings on the Biometra TRIO ..............11 Technical condition ....................11 Requirements for the operating personnel ............... - Page 4 Contents Biometra TRIO Manual Incubation Mode ....................... 36 Creating, editing and saving programs ................ 39 Programming modes....................39 Create a new program/Use program template ............40 Assign program names ..................... 42 Set heated lid temperature ..................42 Set preheating mode ....................43 Edit parameter or step ....................
- Page 5 Biometra TRIO Manual Contents 11.8 Contact ........................94 Adaptation of programs ....................95 Short Manual ........................96 Fault removal ......................... 96 14.1 Forgotten password ....................100 14.2 Slow heating and cooling ..................100 14.3 Auto restart ......................100 14.4 Auto restart due to unrecognized power failure ............100 14.5 Mirror programs to several thermal cyclers ............
- Page 6 Contents Biometra TRIO Manual Figures Figure 1 Biometra TRIO Thermal Cycler ..............15 Figure 2 Biometra TRIO front view ................21 Figure 3 Biometra TRIO rear view ................21 Figure 4 Biometra TRIO lid open button ..............22 Figure 5 Biometra TRIO keyboard ................
- Page 7 Biometra TRIO Manual Contents Figure 52 Biometra TRIO beeper configuration screen ..........74 Figure 53 Network settings screen, IP settings via static ......... 75 Figure 54 Network settings screen, IP settings via DHCP ........75 Figure 55 Biometra TRIO display brightness settings screen ........76 Figure 56 Biometra TRIO touch screen calibration screen ........
- Page 8 Contents Biometra TRIO Manual Version 03_19...
-
Page 9: Basic Information
Biometra TRIO Basic Information Basic Information User manual notes The Biometra TRIO is intended for operation by qualified specialist personnel observ- ing this operating manual. For the Biometra TRIO, there are 3 different models available. In the text below these devices are collectively called Biometra TRIO. -
Page 10: Intended Use
Basic Information Biometra TRIO Symbol for waste disposal in accordance with WEEE directive: Do not dispose of in household waste CE marking China RoHS marking Intended use The Biometra TRIO is an end-point thermal cycler. It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PCR reac- tion. -
Page 11: Safety Instructions
Biometra TRIO Safety instructions Safety instructions General notes For your own safety and to ensure error-free and safe operation of the Biometra TRIO, please read this section carefully before commissioning. Besides the safety instructions in this user manual and the local safety regulations that apply to the operation of the device, the general applicable regulations regarding acci- dent prevention, occupational health and safety and environmental protection have to be observed and complied with. -
Page 12: Requirements For The Operating Personnel
Safety instructions Biometra TRIO The following has to be observed: The operator must only operate the device in a sound and operationally safe condi- tion. The technical condition must always comply with the legal requirements and regulations. Prior to every use the device must be checked for damage and sound condition. ... -
Page 13: Safety Instructions: Protection Against Explosion And Fire
Biometra TRIO Safety instructions The use of oil between the samples and the sample block is not necessary to achieve an improved heat exchange. However, if you still want to use oil, you should use mineral oil. Do not use silicone oil. ... -
Page 14: Handling Of Samples, Auxiliary And Operating Materials
Safety instructions Biometra TRIO 2.6.4 Handling of samples, auxiliary and operating materials The operator is responsible for the selection of substances used in the process as well as for their safe handling. This is particularly important for radioactive, pathogenic, infectious, poisonous, corrosive or otherwise dangerous substances. For details contact the safety officer responsible for your location. -
Page 15: Function And Setup
Biometra TRIO Function and setup Function and setup Field of Applications Developed in 1983 by Kary Mullis PCR (Polymerase Chain Reaction) is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. The Biometra TRIO is an end-point Thermal Cycler and intend- ed to amplify nucleic acids by repeated cycles of heating and cooling using DNA- Polymerases in a PCR reaction. -
Page 16: Special Features
Function and setup Biometra TRIO The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure occurs during the run the instrument will continue the run when the power returns. In case of long- term power failure (longer than 30 minutes) the instrument keeps the sample block at 4°C(freeze step) and the user can decide to repeat the run with the same samples or to discard them. -
Page 17: User-Specific Quick Start Function
Biometra TRIO Function and setup graphical programming mode. This makes the creation of new programs or editing existing programs fast and easy. 3.3.4 User-specific quick start function Many other thermal cyclers offer a so-called "List of latest programs used". In the list of latest programs used a certain number of PCR programs is collected and offered for quick start. -
Page 18: Glp Compliance
Function and setup Biometra TRIO 3.3.7 GLP compliance The software can manage up to 90 user accounts. By default three different user- levels are available: Administrator, user with generic rights and user with limited rights. However, in a comfortable menu the administrator can configure the settings for each user individually by activating or inactivating single rights. -
Page 19: Installation And Commissioning
Biometra TRIO Installation and commissioning Installation and commissioning Content of delivery Thermal Cycler Mains connector Manual Short Manual Please keep the original packaging material for return shipment in case of servicing. The Biometra TRIO Thermal Cycler shipping box provides a specially developed system with form blocks for a safe transport of this electronic device. -
Page 20: Operation Voltage
Installation and commissioning Biometra TRIO Make sure that the main supply voltage is in accordance with the label above the power connection (see chapter "Operation Voltage" p. 20). Connect the Biometra TRIO Thermal Cycler to a grounded socket. ... -
Page 21: Control Elements
Biometra TRIO Control elements Control elements The Biometra TRIO front view Figure 2 Biometra TRIO front view The Biometra TRIO rear view At the Biometra TRIO backside the Ethernet port, mains plug and mains switch are located. Figure 3 Biometra TRIO rear view Version 03_19... -
Page 22: High Performance Smart Lid (Hpsl)
Control elements Biometra TRIO High Performance Smart Lid (HPSL) To achieve optimum pressure on the tubes the Biometra TRIO Thermal Cycler is equipped with a height adjustable heated lid. After the samples have been placed in the block close the lid. Turn the wheel clockwise Close the lid: until you hear a clicking noise. -
Page 23: The Biometra Trio Software
Biometra TRIO Control elements The Biometra TRIO Software The Biometra TRIO features a 7" color touch screen and a completely new software as user interface. 5.5.1 Keyboard For some applications names, addresses or passwords have to be defined. For these purposes the Biometra TRIO software offers a keyboard to enter characters, digits and special characters. -
Page 24: Keypad
Control elements Biometra TRIO ELETE SINGLE CHARACTER ELETE ALL CHARACTERS ORWARD Note: For some functions, the number of maximum characters is limited. The user names, passwords and program names can have maximal 13 characters and user ini- tials maximal 3 characters. 5.5.2 Keypad In some screens of the Biometra TRIO software digits have to be entered. -
Page 25: Cursor Keys
Biometra TRIO Control elements 5.5.3 Cursor keys The Biometra TRIO software uses lists, tables or graphical representations. Whenever the number of entries becomes higher than the maximum number of steps or entries that can be displayed, the software will show cursor keys for navigation. CROLL UP CROLL RIGHT CROLL DOWN... - Page 26 Control elements Biometra TRIO Command Icon ONFIRM ANCEL OGIN SER MANAGEMENT EW USER SER MANAGEMENT ON/OFF ELETE USER DIT USER ROTOCOL IZARD OLYMERASE OLYMERASE ELETE OLYMERASE EMPERATURE PTIMISATION (TOS) DIT PRIMER CONCENTRATION DIT PRIMER ALCULATE PRIMER AVE ANNEALING TEMPERATURE ROTOCOL IZARD NCUBATION TART INCUBATION...
- Page 27 Biometra TRIO Control elements Command Icon ROGRAMS EW PROGRAM PEN TEMPLATE OPY PROGRAM COPY ALL PROGRAMS ELETE PROGRAM DIT PROGRAM TART PROGRAM HOOSE BLOCK START PROGRAM HOOSE BLOCK START PROGRAM TOP PROGRAM AVE PROGRAM AVE AS AUSE PROGRAM ONTINUE PROGRAM KIP STEP RAPHICAL VIEW PREADSHEET VIEW...
- Page 28 Control elements Biometra TRIO Command Icon NSERT STEP ELETE STEP EMPERATURE INCREMENT IME INCREMENT EATING RATE REHEATING REHEATING OOLS ETTINGS ATE AND TIME UTOMATIC USER LOGOUT EEPER ETWORK ISPLAY BRIGHTNESS ISPLAY DARKER ISPLAY BRIGHTER OUCHSCREEN CALIBRATION ACTORY SETTINGS OCUMENTATION Version 03_19...
- Page 29 Biometra TRIO Control elements Command Icon UN LOG FILE UN LOG FILE OVERVIEW AVE RUN LOG FILE TO AVE ALL RUN LOG FILES IEW RUN LOG FILE IEW PROGRAM OWER OGFILE OG FILE SELF TEST RROR LOG FILE XTENDED SELF TEST TART EXTENDED SELF TEST...
-
Page 30: Operating
Operating Biometra TRIO Operating Power On Self-Test After the instrument is switched on the Biometra TRIO Thermal Cycler passes an initial self- test (power on self-test). While the instrument passes the test the start screen is shown Figure 7 Biometra TRIO start screen For the power on self-test a log file is created and stored in the Thermal Cycler memory (see chapter "Power-On-Logfile"... -
Page 31: Log In
Biometra TRIO Operating Figure 8 Biometra TRIO log in screen Log in After starting the Biometra TRIO Thermal Cycler the log in screen is displayed (see chapter "Log in Screen" p. 30). To log in as existing user press L OGIN OGIN In the next screen press the button to selected a user. -
Page 32: Home Screen
Operating Biometra TRIO Touch the input box P and enter the password using the Biometra TRIO key- ASSWORD board (see chapter "Keyboard" p. 23). The default password for the administrator Ad- min is "Admin". Please note that the password query is case sensitive. Figure 10 Biometra TRIO enter password screen Note: If a user directory is not password protected the software skips the enter pass-... -
Page 33: Block Status
Biometra TRIO Operating Command Function Chapter Viewing, running and editing programs See chapter 7 ROGRAMS Automatic creation of PCR programs See chapter 10 ROTOCOL IZARD Shows the current block status. If a block is Free a See chapter 6.5 LOCK touch on the button leads to a user- specific list of the and chapter 6.6 previously used programs... -
Page 34: Quick Start Function
Operating Biometra TRIO Button Block Status Color Function Free Green Quick start function Running Yellow Program view Pause Blue Program view Quick start function Note: Programs can only be started by users with the right S acti- TART STOP PROGRAMS vated (see chapter "Edit user"... -
Page 35: Figure 13 Biometra Trio Quick Start Screen
Biometra TRIO Operating Figure 13 Biometra TRIO quick start screen To switch between the sample blocks choose the number of the sample block. AMPLE BLOCK AMPLE BLOCK AMPLE BLOCK Select and touch a program in the table for quick start. Press the [S ] button to TART start the selected program. -
Page 36: Incubation Mode
Operating Biometra TRIO Figure 14 Biometra TRIO multiblock selection query If necessary, the program can be edited before or a new program can be created using a template (see chapter "Create a new program/Use program template" p. 40). Note: For programs including a TOS step (see chapter "Set Temperature Optimisation Step (TOS)"... -
Page 37: Figure 15 Biometra Trio Incubation Mode Screen
Biometra TRIO Operating Note: To set the time to endless use the following button: NDLESS TIME To select a sample block press: AMPLE BLOCK AMPLE BLOCK AMPLE BLOCK To start the incubation press the [ ] button. START INCUBATION TART INCUBATION Figure 15 Biometra TRIO incubation mode screen During incubation the following commands can be used:... - Page 38 Operating Biometra TRIO The button [ ] for multiblock selection is only available if CHOOSE BLOCK STOP PROGRAM more than one sample block is running. Note: To stop the incubation, use the corresponding command. Do not switch off the instrument while the incubation is running. The instrument will react like after a pow- er failure and will restart the incubation step when it is switched on next time.
-
Page 39: Creating, Editing And Saving Programs
Biometra TRIO Creating, editing and saving programs Creating, editing and saving programs Programming modes The Biometra TRIO software offers spreadsheet programming or graphical program- ming. Use the following buttons to toggle between the two modes (see Figure 16 and Figure 17). RAPHICAL VIEW PREADSHEET VIEW The following screenshots show the spreadsheet and graphical programming mode. -
Page 40: Create A New Program/Use Program Template
Creating, editing and saving programs Biometra TRIO Both programming modes show the same information but in different ways. In the spreadsheet mode all parameters are summarized in a table. In the graphical mode for some parameters symbols are displayed (see table below). Toggle to the spreadsheet mode to view the settings for these parameters. -
Page 41: Figure 18 Biometra Trio Program Overview Screen
Biometra TRIO Creating, editing and saving programs Figure 18 Biometra TRIO program overview screen 1. In the program overview screen press the following button: EW FROM TEMPLATE 2. The program template screen opens (see Figure 18). Search for a program tem- plate using the cursor keys and touch the button to select it. -
Page 42: Assign Program Names
Creating, editing and saving programs Biometra TRIO gram preview table. By using the program preview function the template can be checked before it is opened. 3. Press the [ ] button to open a program template. OPEN TEMPLATE PEN TEMPLATE Figure 20 Biometra TRIO program template screen Assign program names... -
Page 43: Set Preheating Mode
Biometra TRIO Creating, editing and saving programs Figure 21 Biometra TRIO lid temperature setting screen NOTICE The heated lid automatically switches off at a temperature difference of >75 °C be- tween the block and the heated lid. At these low block temperatures, sample conden- sation on the tube lid is no longer to be expected. -
Page 44: Edit Parameter Or Step
Creating, editing and saving programs Biometra TRIO Edit parameter or step The software offers two different options to edit program steps or single parameters: 1. In the programming spreadsheet or the graphical view single parameters can be activated and values entered. 2. -
Page 45: Set Cycles
Biometra TRIO Creating, editing and saving programs Figure 22 Biometra TRIO edit step screen Note: To program a step at least the temperature and the hold time must be defined. To set the temperature touch the corresponding input box and enter a value between 3.0°C and 99.0°C. -
Page 46: Set Temperature Increment
Creating, editing and saving programs Biometra TRIO Figure 23 PCR program with loop In this example the instrument first executes step 1 to step 4, then repeats step 2 to step 4 for 34 times, performs the final extension step at 72°C and holds the tempera- ture indefinitely at 16°C. -
Page 47: Adjust Ramp Rate
Biometra TRIO Creating, editing and saving programs 7.6.4 Adjust ramp rate The average ramp rate can be adjusted for each step. The ramp rate adjustment can be useful if PCR programs are transferred from slower Thermal Cyclers the Biometra TRIO or for special PCR applications like for example telomerase PCR. The average ramp rate specifies the speed at which the selected step is reached with. -
Page 48: Figure 24 Biometra Trio Temperature Optimisation Step Screen
Creating, editing and saving programs Biometra TRIO Figure 24 Biometra TRIO Temperature Optimisation Step screen Version 03_19... -
Page 49: Insert Step
Biometra TRIO Creating, editing and saving programs Insert step To insert a new step, select a program step in the Biometra TRIO programming screen (see Figure 16 and Figure 17) and press the [ ] button. INSERT STEP NSERT By using the [I ] button the E function (see chapter "Edit parameter or NSERT... -
Page 50: Figure 25 Program Overview Screen
Creating, editing and saving programs Biometra TRIO Note: If a new program is to be saved, the first free storage location in the directory of the currently logged in user is automatically selected. However, the storage location can be changed using the cursor keys above and below the lists. If the selected storage location is already in use, the stored program will not be overwritten;... -
Page 51: Figure 26 Quick Selection Screen
Biometra TRIO Creating, editing and saving programs Figure 26 Quick selection screen Before saving the program there is still the possibility of changing the program name. To do this, press the [S ] button and enter the new program name using the Bi- AVE AS ometra TRIO keyboard (see section "Assign program names"... -
Page 52: Starting, Copying And Deleting Programs
Starting, Copying and Deleting programs Biometra TRIO Starting, Copying and Deleting programs Start program Note: Programs can only be started by users with the right S acti- TART STOP PROGRAMS vated (see chapter "Edit user" p. 81). Additionally the right R defines, EAD ALL PROGRAMS if a user is allowed to read all programs or if the access is limited to own programs. -
Page 53: Copy Program
Biometra TRIO Starting, Copying and Deleting programs For the selected program a preview opens with general information on the program and a list of the steps (see Figure 28). The program preview can be used to check the program prior to start. To start the selected program press: TART ROGRAM Figure 28... -
Page 54: Figure 30 Biometra Trio Program Overview Screen
Starting, Copying and Deleting programs Biometra TRIO NABLE A USER TO EDIT COPY AND DELETE OWN PROGRAMS NABLE A USER TO EDIT COPY AND DELETE ALL PROGRAMS OF OTHER USERS To copy a program, touch the corresponding buttons to select a user directory and a stored program in the Biometra TRIO program overview screen (see Figure 18). -
Page 55: Copy All Programs
Biometra TRIO Starting, Copying and Deleting programs Copy all programs Note: Own programs can only be copied and saved by users with the right activated. Programs of others can be copied and saved RITE DELETE OWN PROGRAMS with the right W activated (see chapter "Edit user"... -
Page 56: Delete Program
Starting, Copying and Deleting programs Biometra TRIO Delete program Note: Own programs can only be deleted by users with the right W RITE DELETE OWN activated. Programs of others can be deleted with the right W PROGRAMS RITE DELETE ALL activated (see chapter "Edit user"... -
Page 57: Figure 33 Biometra Trio Security Query
Biometra TRIO Starting, Copying and Deleting programs NABLE A USER TO EDIT COPY AND DELETE ALL PROGRAMS OF OTHER USERS To delete all programs, touch the corresponding button to select a user directory in the Biometra TRIO program overview screen (see Figure 30). If the number of user direc- tories is higher than can be maximal displayed in the list, the buttons above and below the user can be used to scroll up and down. -
Page 58: Running, Pausing, Continuing And Stopping Programs
Running, pausing, continuing and stopping programs Biometra TRIO Running, pausing, continuing and stopping programs To switch between the view for the sample blocks during run select the number of the sample block in the corresponding screen. AMPLE BLOCK AMPLE BLOCK AMPLE BLOCK Display during run After a program is started (see chapter "Display during run"... -
Page 59: Pause Program
Biometra TRIO Running, pausing, continuing and stopping programs Figure 35 Biometra TRIO run screen in spreadsheet view. The following parameters are summarized in the headline table: Program Name Estimated End Remaining Time Sample Block Temperature Lid Temperature ... -
Page 60: Continue Program
Running, pausing, continuing and stopping programs Biometra TRIO Spreadsheet view Graphical view Figure 36 Biometra TRIO run screen during program pause Note: To press the pause button requires manual interaction. The Biometra TRIO software also allows to program a pause step (see chapter "Edit parameter or step" p. -
Page 61: Stop Program
Biometra TRIO Running, pausing, continuing and stopping programs Stop Program Note: Programs can only be stopped by users with the right S acti- TART STOP PROGRAMS vated (see chapter "Edit user" p. 81). NABLE A USER TO START OR STOP PROGRAMS To stop a program press the following button in the Biometra TRIO run screen (see Figure 34). -
Page 62: Protocol Wizard
Protocol Wizard Biometra TRIO Protocol Wizard The protocol wizard is a helpful tool for automatic creation of PCR programs and pri- mer annealing temperature calculation. To start the protocol wizard press the protocol wizard button in the Biometra TRIO home screen (see chapter "Home Screen" p. 32). ROTOCOL IZARD 10.1 Select Polymerase... -
Page 63: Figure 40 Biometra Trio Screen For Editing Program Templates For Polymerases
Biometra TRIO Protocol Wizard OLYMERASE OLYMERASE In the next screen (see Figure 40) touch the corresponding input boxes to enter a name for the polymerase and set parameters for all requested program steps. With two exceptions for most program steps the temperature and the holdtime must be defined: ... -
Page 64: Delete Polymerase
Protocol Wizard Biometra TRIO Figure 41 Biometra TRIO for saving program templates for polymerases Note: If a new or edited polymerase is to be saved, the first free storage place in the polymerase list is pre-selected. However, the storage place can be modified by using the cursor key function above and below the list. -
Page 65: Protocol Wizard Parameter Screen
Biometra TRIO Protocol Wizard 10.4 Protocol Wizard parameter screen For automatic calculation of PCR programs in the protocol wizard parameter screen (see Figure 43) some parameters have to be set by the user: 1. Touch the corresponding checkbox to select between two step and three step pro- grams. -
Page 66: Primer Annealing Temperature Calculator
Protocol Wizard Biometra TRIO The next screen lists the all steps of the program that will be created by the software: Figure 44 Biometra TRIO protocol wizard view parameter screen Press the [ ] button to enter the Biometra TRIO programming screen CREATE PROGRAM (see chapter "Creating, editing and saving programs"... -
Page 67: Figure 46 Biometra Trio Primer Annealing Temperature Calculator Screen
Biometra TRIO Protocol Wizard ometra TRIO protocol wizard parameter screen (see Figure 43) to access the primer annealing temperature calculator screen (see Figure 44). In the annealing temperature calculator screen enter the sequences for the forward and reverse primer using the button below the input fields. The sequence length is counted by the software and displayed in brackets left to the input fields. -
Page 68: Figure 47 Biometra Trio Screen For Primer Concentration Adjustment
Protocol Wizard Biometra TRIO Figure 47 Biometra TRIO screen for primer concentration adjustment Press the [ ] button to toggle to the primer annealing temperature calculator EDIT PRIMER screen (see Figure 47). DIT PRIMER Or press the [ ] button to calculate the theoretical primer annealing tem- CALC PRIMER perature (T... - Page 69 Biometra TRIO Protocol Wizard = ((w*A + x*T)*2) + ((y*G + z*C)*4) where w, x, y and z are the number of the bases A, T, G and C in the primer se- quence, respectively. 2. The average T value is calculated from the T values for both primer sequences 3.
-
Page 70: Tools
Tools Biometra TRIO Tools The Biometra TRIO software provides the following tools: Define basic instrument settings See chapter 11.1 ETTINGS Tool for the user management See chapter 11.2 ANAGEMENT Access to stored log files See chapter 11.3 OCUMENTATION Execute extended self-test See chapter 11.4 XTENDED (SINF) -
Page 71: Set Date And Time
Biometra TRIO Tools Set date and time See chapter 11.1.1 ATE AND Switch automatic user logout on or off See chapter 11.1.2 UTOMATIC USER and time settings LOGOUT Switch beeper on or off See chapter 11.1.3 EEPER Define network settings or list net- See chapter 11.1.4 ETWORK work users... -
Page 72: Automatic User Logout
Tools Biometra TRIO Figure 50 Biometra TRIO date and time screen Activate the corresponding input box and use the keypad on the right side of the date and time screen to enter digits. Additionally the following keys might be helpful to fill out the input boxes: ELETE SINGLE CHARACTER ELETE ALL CHARACTERS... -
Page 73: Configure Beeper
Biometra TRIO Tools UTOMATIC USER LOGOUT If the automatic user logout is switched ON the time after that the user is automatical- ly logged out can be set. You can enter values between 30 minutes and 60 minutes. Figure 51 Biometra TRIO automatic user logout screen Note: The standard setting is automatic user logout OFF. -
Page 74: Network
Tools Biometra TRIO Figure 52 Biometra TRIO beeper configuration screen 11.1.4 Network The N menu item can be used to configure basic settings or show a list of net- ETWORK work users. Press the corresponding button: ETWORK SETTINGS ETWORK USER Note: The network settings can only be configured by users with the right S Network settings YSTEM... - Page 75 Biometra TRIO Tools Figure 53 Network settings screen, IP settings via static Figure 54 Network settings screen, IP settings via DHCP Note: The network cable must be at least performance class Cat 5e and the cable con- figuration has to be STP. The software stores the users who access the Biometra TRIO via the network and lists Network users them in chronological order in a table.
-
Page 76: Display Brightness
Tools Biometra TRIO Copy PCR protocols from the terminal to a selected thermal cycler Read device-specific parameters Read out thermal cycler error messages 11.1.5 Display brightness Note: The display brightness can only be edited by users with the right “S YSTEM ”... -
Page 77: Factory Settings
Biometra TRIO Tools To calibrate the touch screen follow the instructions and touch the screen at the center of the displayed circle. The procedure is repeated three times at different positions of the display. If at the end of the procedure a small cross is shown within the circle the calibration is successful. -
Page 78: User Management
Tools Biometra TRIO 11.2 User Management The Biometra TRIO thermal cycler manages up to 90 individual user accounts. The user management allows to create new users and to set rights for users. The user management is a complex tool. Please follow the general guidelines: 1. - Page 79 Biometra TRIO Tools Figure 59 Biometra TRIO configure user management screen If the user management is switched off, all new users have all rights and can access all software functions. With the user management switched on, the Biometra TRIO Ther- mal Cycler supports 3 different user groups with pre-defined rights: Administrator User with generic...
-
Page 80: Create User
Tools Biometra TRIO User with generic rights User with limited rights User defined If more than 8 user accounts are available you might need to scroll the table up or down to get your desired user account (see Figure 53). CROLL UP CROLL DOWN To select a user account press on the corresponding line in the table. -
Page 81: Edit User
Biometra TRIO Tools Figure 60 Biometra TRIO create user screen Note: The password protection is optional. However, if no password is used, your ac- count containing all of your programs might be modified or deleted by unauthorized persons. Note: The language settings are user specific. For each user the language setting be- comes saved by the instrument. - Page 82 Tools Biometra TRIO Figure 61 Biometra TRIO user data screen Note: For the administrator “Admin” the user name and initials “ADM” are fixed and cannot be modified. It is highly recommendable to change the password for the ad- ministrator „Admin“ after the first system start und to create a backup file. Directories, programs users and right settings can be restored from a backup file.
- Page 83 Biometra TRIO Tools Figure 62 Biometra TRIO user properties screen Right Function Provides access to several system settings like date and time, screen saver, beeper, network YSTEM CONFIGURATION settings, display brightness, touch screen calibration and factory settings Allows to modify the user name, user initials, password, language setting and user rights DIT USER Allows to create new user accounts.
-
Page 84: Delete User
Tools Biometra TRIO 11.2.3 Delete user Note: User accounts can only be deleted by users with the right D activated ELETE (see chapter "Edit user" p. 81). NABLE A USER TO DELETE A USER To delete a user select the user account in the user management screen (see chapter "User Management"... -
Page 85: Run-Logfile
Biometra TRIO Tools Figure 64 Biometra TRIO documentation screen Press the corresponding button to access the desired function. 11.3.1 Run-Logfile The Biometra TRIO Thermal Cycler lists up to 6 Run-Logfiles for the latest programs. Figure 65 Biometra TRIO Run-Logfile overview screen The Run-Logfiles are sorted by date and time. - Page 86 Tools Biometra TRIO In the Run-Logfile screen the following information is listed: Run-Logfile name Serial number Cycler Log time and date Block type Start and end time of the program run Block serial number ...
-
Page 87: Power-On-Logfile
Biometra TRIO Tools Figure 67 Biometra TRIO View program screen Use the following buttons to toggle between the overview screen, view Run-Logfile screen and view program screen. VERVIEW OGFILES OGFILE ROGRAM To export Run-Logfiles to USB press: AVE ALL OGFILES TO Select a logfile from the list and press the following button in the Run-Logfile overview screen (see Figure 66). -
Page 88: Extended Self-Test-Logfile
Tools Biometra TRIO Figure 68 Biometra TRIO Power-On-Logfile screen 11.3.3 Extended Self-Test-Logfile During the Extended Self-Test the Biometra TRIO Thermal Cycler checks several func- tions and components like cooler, thermal tracking, heating and cooling rate, refrig- eration, heated lid and regulation (see figure below). Press on the corresponding button to see the results for a specific test summarized in tabular format. -
Page 89: Error-Logfile
Biometra TRIO Tools 11.3.4 Error-Logfile The Biometra TRIO Thermal Cycler records all errors by time and date. The latest error is shown in position in the Error Log table. Additionally the error code and error mes- sage are listed. Figure 70 Biometra TRIO Error-Logfile screen 11.4 Extended Self-Test NOTICE... - Page 90 Tools Biometra TRIO Test Function Checks, if the sample block reaches the set gradient temperatures. RADIENT Tests, if the heated lid reaches the set temperature and if it holds the EATED LID temperature for a longer time. Regulation Tests, if the sample block is controlled properly. not available for Biometra TRIO In certain intervals the Biometra TRIO software will automatically recommend to exe- cute the Extended Self-Test.
- Page 91 Biometra TRIO Tools Figure 72 Biometra TRIO Extended Self-Test screen During the test the software provides a progress overview. If a test is already passed the result “Failed” or “Passed” is displayed, for the currently performed test “Running” is shown and all other tests are not finished yet. Note: The Extended Self-Test will take approximately 30 minutes to finish and the instrument is blocked, no other program can be started while the test is running.
-
Page 92: Service Info File (Sinf)
Tools Biometra TRIO 11.5 Service Info File (SINF) The Service Info File is a helpful tool for remote failure diagnosis by the Biometra ser- vice department. Figure 74 Biometra TRIO create Service Info File screen To create a Service Info File connect a USB stick to the Biometra TRIO Thermal Cycler and press: NFO FILE TO The Service Info File can be send by e-mail to the Biometra service department. -
Page 93: Cycler Info
Biometra TRIO Tools Figure 75 Biometra TRIO backup file screen To load a backup file select a backup from the list and press: OAD BACKUP FILE Backup files that are no longer used can be deleted by using the functions ELETE ALL ACKUP ILES... -
Page 94: Contact
Tools Biometra TRIO Figure 76 Biometra TRIO cycler info screen 11.8 Contact Shows contact details for the Biometra service department. Figure 77 Biometra TRIO contact details screen Version 03_19... -
Page 95: Adaptation Of Programs
Biometra TRIO Adaptation of programs Adaptation of programs If programs are exchanged between different thermal cycler models or of the sample block is exchanged programs might need to be adapted prior to start or during editing. The following table provides an overview for possible adaptations: Cause Example Adjustment... -
Page 96: Short Manual
Short Manual Biometra TRIO Short Manual WARNING Before initial operation check the correct operating voltage is set on the voltage selec- tion switch on the underside of the device. Do not open the housing! Danger of elec- tric shock! CAUTION The sample block and the heated lid can reach high temperatures during operation. - Page 97 Biometra TRIO Short Manual Press [I ] to incubate the sample block at a constant temperature NCUBATE Press [T ] to access the system settings, user management, documentation, OOLS backup tool or self-test functions Press [L ] to log out OGOUT Important note: It depends on your rights settings if you are allowed to create, delete, Edit, save and copy pro-...
- Page 98 Short Manual Biometra TRIO Use the cursor keys at the top of the screen to switch between program steps. The number of the currently activated step is displayed. Touch the input box “T ” and enter a temperature (°C). EMPERATURE ...
- Page 99 Biometra TRIO Short Manual Save program Copy/Delete programs To copy programs press the button [P ] in the Biometra TRIO home screen. ROGRAMS To copy all programs located in a user directory select a user directory and press the button [C ].
-
Page 100: Forgotten Password
Fault removal Biometra TRIO Fault removal 14.1 Forgotten password Passwords can be changed by the systems administrator(s) and by users with the acti- vated right E (see chapter "Edit user" p. 81). If the administrator Admin has forgotten the password and no one else has the right E use the function DIT USER (see chapter "Factory settings"... -
Page 101: Mirror Programs To Several Thermal Cyclers
Biometra TRIO Fault removal 14.5 Mirror programs to several thermal cyclers Using the backup function, complete thermal cyclers can be mirrored. Copy complete users (via user management) and their programs from one thermal cycler to the other without having to create each individual user or program. The prerequisite is that both cyclers have the same software version (see chapter 11.7). -
Page 102: Maintenance And Repair
Maintenance and Repair Biometra TRIO Maintenance and Repair 15.1 Factory calibration This instrument is supplied fully calibrated by the manufacturer before delivery, using equipment traceable to national standards. The Biometra Quality Management- System is certified by BSI for BS EN ISO 9001:2015 and the used measurement system is tested by an accredited testing laboratory in accordance with EN ISO 17025 at pre- scribed intervals and gets regularly calibrated. -
Page 103: Disinfecting The Device
Biometra TRIO Maintenance and Repair Only wipe the housing of the Biometra TRIO with a soft clean cloth which may be slightly wetted with a commercial neutral detergent, if necessary. Clean the ventilation slits on the underside and at the rear of the device with a vacuum cleaner. -
Page 104: Service
Service Biometra TRIO Service Should you have any problems with this unit, please contact our service department or your local Biometra dealer. Note: If you would like to send the unit back to us, please read the following return instructions. 16.1 Instructions for return shipment WARNING Risk of damage to health due to improper decontamination! Perform a professional... -
Page 105: Packing Of The Biometra Trio Thermal Cycler
Biometra TRIO Service 16.2 Packing of the Biometra TRIO Thermal Cycler Biometra uses an extra designed packaging system with inserts of foamed material. NOTICE The Thermal Cycler is only protected from transport damage if the original transport box is used and the packing instructions are followed. Biometra is not responsible for transport damage by improper packing. -
Page 106: Disposal
Disposal Biometra TRIO Disposal The operator of the Biometra TRIO must dispose of any waste materials that occur during measurements (sample materials) in accordance with statutory and local regu- lations. At the end of its service life, the Biometra TRIO and all its electronic components must be disposed of as electronic waste in accordance with valid regulations. -
Page 107: Technical Specifications
Biometra TRIO Technical specifications Technical specifications Order Number 846-x-070-720 846-x-070-723 846-x-070-724 Name Biometra TRIO 30 Biometra TRIO 48 Biometra TRIO combi Capacity 3 blocks for 30 x 0.5ml 3 blocks for 48 x 0.2ml 3 combi blocks for 18 x tubes each tubes 0.5ml tubes**... - Page 108 Technical specifications Biometra TRIO Order Number 846-x-070-720 846-x-070-723 846-x-070-724 Working conditions 15 °C to 35 °C, 70 % air humidity, max 2000 m NN Dimensions (WxDxH) 30 cm x 41 cm x 25 cm Weight 17.3 kg * Measured inside the block ** Capacity increases to 35 x 0.5 ml tubes by use of small cap tubes 1 The network cable must be at least performance class Cat 5e and the cable configuration has to be STP x = 2 for 230 V, 4 for 115 V, 5 for 100 V...
-
Page 109: Declaration Of Conformity
Biometra TRIO Declaration of Conformity Declaration of Conformity Version 03_19... -
Page 110: Index
Index Biometra TRIO Index Auto restart 99 Graphical Programming 39 Backup 91 Heated lid temperature 42 Block status 33 Heating/Cooling rate 46 Care 101 Insert or delete step 48 Clean the housing 102 Loop 45 Commands 25 Name 42 Contact 93 New 40 Decontamination 102 Overview 51...
Need help?
Do you have a question about the Analytik Jena Biometra TRIO Series and is the answer not in the manual?
Questions and answers