Endress+Hauser Analytik Jena SpeedMill PLUS Operating Manual
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Endress+Hauser Analytik Jena SpeedMill PLUS Operating Manual

Homogenizer
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Operating Manual
SpeedMill PLUS
Homogenizer

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Summary of Contents for Endress+Hauser Analytik Jena SpeedMill PLUS

  • Page 1 Operating Manual SpeedMill PLUS Homogenizer...
  • Page 2 Manufacturer Analytik Jena GmbH Konrad-Zuse-Strasse 1 07745 Jena / Germany Phone: +49 3641 77 70 Fax: +49 3641 77 9279 Email: info@analytik-jena.com Technical Service Analytik Jena GmbH Konrad-Zuse-Strasse 1 07745 Jena / Germany Phone: +49 3641 77 7407 Fax: +49 3641 77 9279 Email: service@analytik-jena.com For a proper and safe use of this product follow the instructions.

  • Page 3: Table Of Contents

    SpeedMill PLUS Table of contents Table of contents 1 Basic information................................About this user manual ............................ Intended use ..............................2 Security .................................... Safety labeling on the device ........................... Requirements for the operating personnel..................... Safety instructions for transport and commissioning..................Safety instructions: during operation ......................2.4.1 General ................................
  • Page 4 Table of contents SpeedMill PLUS 7 Transport and storage ..............................33 Preparing devices for transport ........................33 Transport................................34 Storage ................................34 8 Disposal ................................... 35 9 Technical data ................................. 36...

  • Page 5: Basic Information

    SpeedMill PLUS Basic information Basic information About this user manual Content This user manual describes the following homogenizer: ¡ SpeedMill PLUS The device is intended to be operated by qualified specialist personnel observing the op- erating manual. The operating manual provides information about the design and operation of the de- vice and provides operating personnel the necessary know-how for the safe handling of the device and its components.
  • Page 6 Basic information SpeedMill PLUS The device can reduce both hard and soft source material. Even very resistant source materials like bones, cartilage, chitinous exoskeleton of insects or ticks can be homoge- nized completely and reproducibly in a short period of time. The homogenizer may only be used for the methods described in these operating in- structions.

  • Page 7: Security

    SpeedMill PLUS Security Security For your own safety and to ensure error-free and safe operation of the device, please read this chapter carefully before commissioning. Observe all safety instructions listed in this user manual and all messages and infor- mation displayed on the monitor by the control and analysis software. Safety labeling on the device Warning and mandatory action labels have been attached to the device and must al- ways be observed.

  • Page 8: Safety Instructions: During Operation

    Security SpeedMill PLUS Risk of damage to health due to improper decontamination! Perform a profes- ¡ sional and documented decontamination of the device before returning it to An- alytik Jena. The decontamination report is available from Service when register- ing the return. Service must refuse acceptance of contaminated devices. The sender may be liable for damage caused by inadequate decontamination of the device.

  • Page 9: Handling Of Auxiliary And Operating Materials And Samples

    SpeedMill PLUS Security plate of the equipment. Do not replace the removable power cable of the device with a power cable that does not meet the specifications (with no protective ground conductor). Extensions of the supply cable are not permitted! ¡...

  • Page 10: Behavior During Emergencies

    Security SpeedMill PLUS Behavior during emergencies ¡ If there is no immediate risk of injury, switch off the device and the connected system components immediately in hazardous situations or in the event of an accident and/or disconnect the power plugs from the power outlets.

  • Page 11: Function And Design

    SpeedMill PLUS Function and design Function and design The SpeedMill PLUS homogenizer can mechanically reduce source material intended for subsequent isolation and purification of DNA, RNA or proteins. As the samples only heat up to a minimal degree, continuous operation is possible. The device can reduce both hard and soft source material.

  • Page 12: Figure 2 Interior View (With Sample Holder)

    Function and design SpeedMill PLUS Figure 2 Interior view (with sample holder) 1 Lid 2 Sample holder The homogenizer is a standalone device. No PC with control and monitoring software is required. Sample holder The sample holder has three components. The three components are inserted in se- quence in the sample chamber.

  • Page 13: Figure 3 Sample Holder, Disassembled

    SpeedMill PLUS Function and design Figure 3 Sample holder, disassembled 1 Base plate 2 Holder for reaction vessels 3 Closure and upper cover Analytik Jena provides special tube fasteners as optional accessories for reaction vessels with mandrels (such as innuSPEED Lysis Tube Q). The tube fasteners replace the stan- dard sample holder.

  • Page 14: Figure 5 Terminals And Switches On The Rear

    Function and design SpeedMill PLUS Terminals and switches on the rear of the device Figure 5 Terminals and switches on the rear 1 Lid 2 Ventilation opening 3 Power connection 4 Mains fuse 5 Power switch 6 Touch sensor switch 7 COM interface (for service) The switch on the rear of the device can be used to active/deactivate the touch sensor.

  • Page 15: Figure 6 Control Elements

    SpeedMill PLUS Function and design Control elements The keyboard, the display and the touch sensor are located on the front side of the ho- mogenizer. Figure 6 Control elements 1 Screen 2 Keyboard 3 Touch sensor with LED status display The keys have the following functions: ▴▾◂▸...

  • Page 16: Installation And Commissioning

    Installation and commissioning SpeedMill PLUS Installation and commissioning Installation conditions Ambient conditions ¡ This laboratory device is designed for inside use. Do not use the device in wet and damp environments. Keep the device surface clean ¡ and dry. The installation site must be free of drafts, dust and caustic fumes. ¡...

  • Page 17: Commissioning

    SpeedMill PLUS Installation and commissioning Commissioning DANGER Risk of electric shock The device is intended for use at 115/230 V. Operating the device with incorrect voltage can result in electric shocks and destruction of the device. ð The operating voltage can be set via the fuse holder. The operating voltage is indi- cated in the viewing window of the fuse holder.
  • Page 18 Installation and commissioning SpeedMill PLUS Switching the device off } When the homogenization process has finished, the device can be switched off via the power switch. } Only switch off the device after the cooling period (at least 30 s) has elapsed. The device may overheat otherwise.

  • Page 19: Operation

    SpeedMill PLUS Operation Operation Preparing the samples Observe the following instructions when preparing the samples and operating the ho- mogenizer: ¡ Hazardous substances such as strong acids or alkaline solutions must not be han- dled by the device, as these can damage both the device and the reaction vessels. Combustible liquids or substances that can form explosive compounds must also not be handled by the device.

  • Page 20: Inserting Samples In The Sample Holder

    Operation SpeedMill PLUS Preparing samples with other reaction vessels CAUTION Risk of injury from broken glass Glass vessel can break during homogenization. ¡ Only use plastic reaction vessels. Observe the following when selecting reaction vessels: Use plastic reaction vessels with screw-on caps. ¡...
  • Page 21 SpeedMill PLUS Operation } Insert the holder. Press the holder down until its edge lies flush with the edge of the sample chamber. } Load the holder with reaction vessels. Balance the reaction vessels. } Place the closure on the pin. } Close the sample holder.

  • Page 22: Removing Samples

    Operation SpeedMill PLUS Removing samples } Press the closure down and turn the closure counterclockwise by 90°. NOTICE! Do not attempt to pull the closure off toward the top before unlocking. } Remove the closure. } Remove the reaction vessels with the samples. Homogenizing the samples } Cool the sample holder down to the desired temperature.

  • Page 23: Interval Mode

    SpeedMill PLUS Operation } Enter the Prep time duration as "Minutes:Seconds". – Switch from minutes to seconds with the ◂▸ arrow keys. – Enter the time in increments with the ▴▾ arrow keys. } Start homogenization with [enter]. ü The time is displayed running backwards. The following text appears: Press esc to stop.
  • Page 24 Operation SpeedMill PLUS } Enter the next interval step. } After entering the interval step: Select one of the following options with the arrow keys: – Go back to the previous interval step with Back. – Accept the protocol with Accept. –...

  • Page 25: Quick Mode

    SpeedMill PLUS Operation 5.5.3 Quick mode Quick mode lets you start homogenization immediately without making any settings. You can set the homogenization time for Quick mode in the Settings menu (→ "Operat- ing mode presets"  25). If no settings are made, Quick mode uses the time from the last homogenization.

  • Page 26: Quick Mode Settings

    Operation SpeedMill PLUS } Select Continuous mode and confirm via [enter] } Set the homogenization time Prep time with the arrow keys and confirm via [enter]. } Answer the question Use as default time?. Move the cursor to the yes op- tion with the arrow keys for this.

  • Page 27: Managing Protocols

    SpeedMill PLUS Operation Managing protocols Protocols are homogenization processes in Interval mode. These can contain one or more intervals that are repeated multiple times. The user can create their own protocols. ¡ The device is supplied with a selection of preset protocols. ¡...

  • Page 28: Starting Protocols

    Operation SpeedMill PLUS } Select one of the following two folders with the arrow keys: – pre-programmed: Folder with preinstalled protocols – user-defined: Folder with user-defined protocols } Confirm your selection with [enter]. ü The list of saved protocols appears. } Select a protocol.

  • Page 29: Editing Protocols

    SpeedMill PLUS Operation After the protocol has finished, Program finished is displayed. } Confirm via [enter]. } Press [enter], to start the protocol once more. } Or: Press [esc] to return to the main menu. 5.7.4 Editing protocols The user can only change their own protocols. Preset protocols saved in the pre-pro- grammed folder cannot be edited by the user.

  • Page 30: Preset Protocols

    Operation SpeedMill PLUS } Confirm the security query via [enter]. ü The protocol is deleted. Preset protocols The device is supplied with preset protocols. Various source materials can be optimally homogenized with the aid of these protocols. The protocol names have been selected in accordance with the source materials and the hardness (MH: medium hard, VH: very hard).

  • Page 31: Maintenance And Care

    SpeedMill PLUS Maintenance and care Maintenance and care WARNING Risk of electric shock ¡ Always switch off the device and disconnect the power plug before performing maintenance work. Power to the device is only disconnected by disconnecting the power plug. After the device is switched off, some parts are still live.

  • Page 32: Switching The Operating Voltage

    Maintenance and care SpeedMill PLUS } Pull out the fuse block. } Replace the faulty mains fuse. Use the following fuses: 2  x T 4 A / 250 V. } Reinsert the fuse holder. WARNING! Insert the fuse block in the correct direction. The marking for the de- sired operating voltage must point to the right.

  • Page 33: Transport And Storage

    SpeedMill PLUS Transport and storage Transport and storage Preparing devices for transport NOTICE Risk of device damage due to unsuitable packaging material ¡ Only transport the device and its components in the original packaging. ¡ Empty the device completely and attach all transport locks before transporting the device.

  • Page 34: Transport

    Transport and storage SpeedMill PLUS Transport When transporting the device, observe the safety instructions in the "Safety instructions" section. Avoid the following during transport: ¡ Impact and vibration Risk of damage due to shock, impact or vibration! Large temperature fluctuations ¡...

  • Page 35: Disposal

    SpeedMill PLUS Disposal Disposal The operator is responsible for correct disposal of biological samples in accordance with statutory regulations. At the end of its service life, the device and its electronic components must be dis- posed of as electronic waste in accordance with the applicable regulations.

  • Page 36: Technical Data

    Technical data SpeedMill PLUS Technical data System parameters Homogenization time 30 s to 4 min (dependent on source material type) Oscillation speed 50 Hz Acceleration time none Deceleration time none Volume (max.) 74 dB Application parameters Sample capacity Parallel processing of up to 20 samples Sample cooling Passively cooled sample holder, storage until -80 °C Program parameters Homogenization time...
  • Page 37 SpeedMill PLUS Table of figures Table of figures Figure 1 Front view................................11 Figure 2 Interior view (with sample holder) ........................12 Figure 3 Sample holder, disassembled ..........................13 Figure 4 Tube fasteners with innuSPEED Lysis Tube Q (with mandrels) ................ 13 Figure 5 Terminals and switches on the rear........................

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