Endress+Hauser Analytik Jena Biometra TRIO Operating Manual
  1. Manuals
  2. Brands
  3. Endress+Hauser Manuals
  4. Laboratory Equipment
  5. Analytik Jena Biometra TRIO
  6. Operating manual
Endress+Hauser Analytik Jena Biometra TRIO Operating Manual

Endress+Hauser Analytik Jena Biometra TRIO Operating Manual

Multi-block thermal cycler three pcr devices in one
Hide thumbs Also See for Analytik Jena Biometra TRIO:
Table of Contents

Advertisement

Quick Links

Download this manual
Operating Manual
Biometra TRIO
Multi-Block Thermal Cycler
Three PCR Devices in One

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Analytik Jena Biometra TRIO and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Endress+Hauser Analytik Jena Biometra TRIO

Summary of Contents for Endress+Hauser Analytik Jena Biometra TRIO

  • Page 1 Operating Manual Biometra TRIO Multi-Block Thermal Cycler Three PCR Devices in One...
  • Page 2 Biometra GmbH (Analytik Jena GmbH) Manufacturer Rudolf-Wissell-Str. 30 37079 Göttingen  Germany Phone + 49 551 50 68 6-0 + 49 551 50 68 6-66 Email Info@analytik-jena.com Biometra GmbH Service Rudolf-Wissell-Str. 15 37079 Göttingen  Germany Phone + 49 3641 777407 Email Service@analytik-jena.com http://www.analytik-jena.com...

  • Page 3: Table Of Contents

    Biometra TRIO Contents Contents Basic Information ....................... 9 User manual notes ......................9 Intended use ........................10 Safety instructions ....................11 General notes ........................11 Safety markings on the Biometra TRIO ................11 Technical condition ......................12 Requirements for the operating personnel ..............12 Safety instructions: transport and installation ...............
  • Page 4 Contents Biometra TRIO Log in ..........................35 Home Screen........................36 Block status ........................37 Quick start function ......................38 Incubation Mode......................41 Creating, editing and saving programs ..............43 Programming modes ....................... 43 Create a new program/Use program template .............. 45 Assign program names....................
  • Page 5 Biometra TRIO Contents 11.3.3 Extended Self-Test-Logfile ..................... 94 11.3.4 Error-Logfile ........................95 11.4 Extended Self-Test ......................95 11.5 Service Info File (SINF) ....................98 11.6 Backup ..........................98 11.7 Cycler Info ........................99 11.8 Contact........................... 100 Adaptation of programs ..................101 Short Manual ......................
  • Page 6 Figures Biometra TRIO Figures Fig. 1 Biometra TRIO Thermal Cycler ................17 Fig. 2 Biometra TRIO front view ..................24 Fig. 3 Biometra TRIO rear view ...................24 Fig. 4 Biometra TRIO lid open button .................25 Fig. 5 Biometra TRIO keyboard ..................26 Fig. 6 Biometra TRIO keypad ..................27 Fig.
  • Page 7 Biometra TRIO Figures Fig. 50 Biometra TRIO date and time screen ............... 78 Fig. 51 Biometra TRIO automatic user logout screen ..........79 Fig. 52 Biometra TRIO beeper configuration screen ............ 80 Fig. 53 Network settings screen, IP settings via static ..........81 Fig.
  • Page 8 Figures Biometra TRIO...

  • Page 9: Basic Information

    Biometra TRIO Basic Information Basic Information User manual notes The Biometra TRIO is intended for operation by qualified specialist personnel observing this operating manual. For the Biometra TRIO, there are 3 different models available. In the text below these devices are collectively called Biometra TRIO. Differences are explained in the corre- sponding sections.

  • Page 10: Intended Use

    Basic Information Biometra TRIO Intended use The Biometra TRIO is an end-point thermal cycler. It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PCR reaction. The thermal cycler is developed as product for general laboratory use. The Biometra TRIO combines modern design with user-friendly software.

  • Page 11: Safety Instructions

    Biometra TRIO Safety instructions Safety instructions General notes For your own safety and to ensure error-free and safe operation of the Biometra TRIO, please read this section carefully before commissioning. Besides the safety instructions in this user manual and the local safety regulations that apply to the operation of the device, the general applicable regulations regarding acci- dent prevention, occupational health and safety and environmental protection have to be observed and complied with.

  • Page 12: Technical Condition

    Safety instructions Biometra TRIO Keep ventilation slits clear Keep the ventilation slits on the underside and at the rear of the device clear. Make sure that there are no objects underneath the device (e.g., paper) that may be sucked against the ventilation slot and thus inter- fere with ventilation.

  • Page 13: Safety Instructions - Operation

    Biometra TRIO Safety instructions Safety instructions – operation 2.6.1 General The operator of the Biometra TRIO must make sure before each commissioning that the condition of the device including the safety equipment is sound. The following has to be observed: ...

  • Page 14: Handling Of Samples, Auxiliary And Operating Materials

    Safety instructions Biometra TRIO  The electrical components must be checked regularly by a qualified electrician. Any defects, such as loose connections, faulty or damaged cables, must be repaired with- out delay.  Before opening the device it must be switched off at the power switch and the mains plug must be disconnected from the mains outlet! ...

  • Page 15: Behavior During Emergencies

    Biometra TRIO Safety instructions  Only use original spare parts, wear parts and consumables. These have been tested and guarantee safe operation. Behavior during emergencies In case of danger or accidents, immediately switch off the Biometra TRIO using the main switch on the equipment back plate.

  • Page 16: Function And Setup

    Function and setup Biometra TRIO Function and setup Field of Applications Developed in 1983 by Kary Mullis PCR (Polymerase Chain Reaction) is now a common and often indispensable technique used in medical and biological research labs for a va- riety of applications. The Biometra TRIO is an end-point Thermal Cycler and intended to amplify nucleic acids by repeated cycles of heating and cooling using DNA-Polymerases in a PCR reaction.

  • Page 17: Special Features

    Biometra TRIO Function and setup Fig. 1 Biometra TRIO Thermal Cycler The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure oc- curs during the run the instrument will continue the run when the power returns. In case of long- term power failure (longer than 30 minutes) the instrument keeps the sample block at 4°C(freeze step) and the user can decide to repeat the run with the same sam- ples or to discard them.

  • Page 18: High Performance Smart Lid

    Function and setup Biometra TRIO 3.3.2 High Performance Smart Lid The Biometra TRIO heated lid has been optimized to heat twice as fast as with older models and thus helps to shorten protocol run times. Moreover the heated lid serves for two additional essential functions: It prevents the formation of condensed water at the reaction tube portion located above the block surface level and it ensures reliable con- tact between samples and thermal block by applying constant pressure.

  • Page 19: Temperature Optimisation Step (Tos)

    Biometra TRIO Function and setup The Biometra TRIO software offers several pre-installed program templates for different applications. The program templates provide a general protocol structure and can be easily adapted for the current experiment. The protocol wizard offers the comfort to create specific PCR programs by just a few in- puts.

  • Page 20: Order Numbers

    Function and setup Biometra TRIO used sealing method thanks to the HPSL technology (see section 3.3.2) always the same pressure is applied to the consumables serving for absolutely reproducible conditions. The Biometra TRIO Thermal Cycler is not limited to reagents from a specific vendor and can be used with any kind of polymerase.

  • Page 21: Installation And Commissioning

    Biometra TRIO Installation and commissioning Installation and commissioning Content of delivery  Thermal Cycler  Mains connector  Manual  Short Manual Please keep the original packaging material for return shipment in case of servicing. The Biometra TRIO Thermal Cycler shipping box provides a specially developed system with form blocks for a safe transport of this electronic device.

  • Page 22: Installation Conditions

    Installation and commissioning Biometra TRIO Installation Conditions 4.4.1 General setup  Place the Biometra TRIO Thermal Cycler on a stable surface in a dry, safe environ- ment. For details see working conditions in table "Technical specifications" (see sec- tion 18 p. 114 ). ...

  • Page 23: Installation When Used In A Network

    Biometra TRIO Installation and commissioning  The display contrast can be adjusted to local light conditions. 4.4.2 Installation when used in a network When using the thermal cycler together with the Biometra TSuite thermal cycler man- agement computer software, please follow these instructions for connection in a net- work: ...

  • Page 24: Control Elements

    Control elements Biometra TRIO Control elements The Biometra TRIO front view Fig. 2 Biometra TRIO front view The Biometra TRIO rear view At the Biometra TRIO backside the Ethernet port, mains plug and mains switch are lo- cated. Fig. 3 Biometra TRIO rear view...

  • Page 25: High Performance Smart Lid (Hpsl)

    Biometra TRIO Control elements High Performance Smart Lid (HPSL) To achieve optimum pressure on the tubes the Biometra TRIO Thermal Cycler is equipped with a height adjustable heated lid. After the samples have been placed in the block close the lid. Turn the wheel clockwise Close the lid: until you hear a clicking noise.

  • Page 26: The Biometra Trio Software

    Control elements Biometra TRIO The Biometra TRIO Software The Biometra TRIO features a 7" color touch screen and a completely new software as user interface. 5.5.1 Keyboard For some applications names, addresses or passwords have to be defined. For these pur- poses the Biometra TRIO software offers a keyboard to enter characters, digits and spe- cial characters.

  • Page 27: Keypad

    Biometra TRIO Control elements Shift Delete single character Delete all characters Back Forward Note: For some functions, the number of maximum characters is limited. The user names, passwords and program names can have maximal 13 characters and user initials maximal 3 characters. 5.5.2 Keypad In some screens of the Biometra TRIO software digits have to be entered.

  • Page 28: Cursor Keys

    Control elements Biometra TRIO 5.5.3 Cursor keys The Biometra TRIO software uses lists, tables or graphical representations. Whenever the number of entries becomes higher than the maximum number of steps or entries that can be displayed, the software will show cursor keys for navigation. Scroll up Scroll right Scroll down...
  • Page 29 Biometra TRIO Control elements Command Icon Confirm Cancel Login User management New user User management ON/OFF Delete user Edit user Protocol Wizard New Polymerase Edit Polymerase Delete Polymerase Temperature Optimi- sation Step (TOS) Edit primer concent- ration Edit primer Calculate primer Save annealing tem- perature Ta Protocol Wizard Info...
  • Page 30 Control elements Biometra TRIO Command Icon Start incubation Programs New program Open template Copy program/copy all programs Delete program Edit program Start program Choose block start program Choose block start program Stop program Done Save program Save as Pause program Continue program Skip step Graphical view...
  • Page 31 Biometra TRIO Control elements Command Icon Edit step Insert step Delete step Temperature incre- ment Time increment Heating rate Preheating OFF Preheating ON Tools Settings Date and time Automatic user lo- gout Beeper Network Display brightness Display darker Display brighter Touchscreen calibra- tion Factory settings...
  • Page 32 Control elements Biometra TRIO Command Icon Documentation Run log file Run log file overview Save run log file to Save all run log files to USB View run log file View program Power-on-Logfile Log file self-test Error log file Extended self-test Start extended self- test Stop extended self-...
  • Page 33 Biometra TRIO Control elements Command Icon Contact...

  • Page 34: Operating

    Operating Biometra TRIO Operating Power On Self-Test After the instrument is switched on the Biometra TRIO Thermal Cycler passes an initial self- test (power on self-test). While the instrument passes the test the start screen is shown Fig. 7 Biometra TRIO start screen For the power on self-test a log file is created and stored in the Thermal Cycler memory (see section "Power-On-Logfile"...

  • Page 35: Log In

    Biometra TRIO Operating Fig. 8 Biometra TRIO log in screen Log in After starting the Biometra TRIO Thermal Cycler the log in screen is displayed (see sec- tion "Log in Screen" p. 34). To log in as existing user press Login. Login In the next screen press the button to selected a user.

  • Page 36: Home Screen

    Operating Biometra TRIO left to bottom right by the date and time of the latest user log in. If more than 6 user ac- counts are available you might need to use the cursor keys Left or Right to turn the page (see section "List of used commands and icons"...

  • Page 37: Block Status

    Biometra TRIO Operating Fig. 11 Biometra TRIO home screen Command Function Section Programs Viewing, running and editing programs See section "Creating, editing and sav- ing programs" p. 43 Protocol Automatic creation of PCR programs See section "Protocol Wizard" p. 68 Wizard Block Shows the current block status.

  • Page 38: Quick Start Function

    Operating Biometra TRIO Block temperature Block status Remaining run time / pause time Fig. 12 Biometra TRIO block status/quick start button The following table summarizes the different button colors and sample block status. When the button is pressed, it depends on the sample block status which function is ac- tivated by the Biometra TRIO software.

  • Page 39: Fig. 13 Biometra Trio Quick Start Screen

    Biometra TRIO Operating Enable a user to start or stop programs The Biometra TRIO software offers a quick start function for previously used programs. To use the quick start function, press the block status button in the log in screen (see section "Log in Screen"...

  • Page 40: Fig. 14 Biometra Trio Multiblock Selection Query

    Operating Biometra TRIO Sample block 3 Select and touch a program in the table for quick start. Press the Start button to start the selected program. Start on block x To start a program on multiple sample blocks press the choose block start program but- ton.

  • Page 41: Incubation Mode

    Biometra TRIO Operating Incubation Mode Note: Incubations can only be started or stopped by users with the right Start/stop pro- grams activated (see section "Edit user" p. 88). Enable a user to start or stop programs The incubation mode allows to incubate samples at a constant temperature for a de- fined time.

  • Page 42: Fig. 15 Biometra Trio Incubation Mode Screen

    Operating Biometra TRIO Fig. 15 Biometra TRIO incubation mode screen During incubation the following commands can be used: Stop Incubation Choose block Pause Incubation Continue Incubation Skip Step The button choose block stop program for multiblock selection is only available if more than one sample block is running.

  • Page 43: Creating, Editing And Saving Programs

    Biometra TRIO Creating, editing and saving programs Creating, editing and saving programs Programming modes The Biometra TRIO software offers spreadsheet programming or graphical program- ming. Use the following buttons to toggle between the two modes (see Fig. 16 and Fig. 17).

  • Page 44: Fig. 17 Biometra Trio Graphical Programming Screen

    Creating, editing and saving programs Biometra TRIO Fig. 17 Biometra TRIO graphical programming screen Both programming modes show the same information but in different ways. In the spreadsheet mode all parameters are summarized in a table. In the graphical mode for some parameters symbols are displayed (see table below).

  • Page 45: Create A New Program/Use Program Template

    Biometra TRIO Creating, editing and saving programs Create a new program/Use program template Note: New programs can only be created by users with the right Write/delete own pro- grams activated (see section "Edit user" p. 88). Programs of other users can only be modified by users with the right Write/delete all programs activated.

  • Page 46: Fig. 19 Biometra Trio Program Template "Blank

    Creating, editing and saving programs Biometra TRIO Note: The Biometra TRIO offers several program templates for different purposes. You may use a template and modify the program as desired or you can select Blank. Blank is program with no pre-defined steps (see Fig. 19). Spreadsheet programming Graphical programming Fig.

  • Page 47: Assign Program Names

    Biometra TRIO Creating, editing and saving programs Assign program names Each template has a specific name which is superimposed in the programming screen. To enter a new name touch the input box Name and enter a new name using the Bi- ometra TRIO virtual keyboard (see section Keyboard""...

  • Page 48: Set Preheating Mode

    Creating, editing and saving programs Biometra TRIO Set preheating mode The heated lid can be set to preheating On or Off using the corresponding buttons: Preheating Off Preheating On If the preheating mode is activated the heated lid is heated up to the target temperature while the sample block is held constant at 25°C.

  • Page 49: Fig. 22 Biometra Trio Edit Step Screen

    Biometra TRIO Creating, editing and saving programs Time increment (e.g. for long range PCR). Increases the set time See section "Set time incre- with each cycle by the defined increment. The increment can be ment" p. 51 set from 0s to 240s. Average ramp rate between steps.

  • Page 50: Set Cycles

    Creating, editing and saving programs Biometra TRIO Next step The currently activated step is high-lighted in the program overview table (see Fig. 22). Note: By navigating between steps it is not necessary to confirm the settings for each single step and to leave the edit step screen but you can fill in the parameters for several steps and confirm the settings after all steps are programmed.

  • Page 51: Set Temperature Increment

    Biometra TRIO Creating, editing and saving programs 7.6.2 Set temperature increment For some applications, like for example touch down PCR, temperature increments are used. The temperature is increased or decreased with each cycle by the set value. To program a temperature increment press the following button (see Fig. 22) and fill in a value between +20°C and -20°C in the corresponding input box.

  • Page 52: Set Temperature Optimisation Step (Tos)

    Creating, editing and saving programs Biometra TRIO 7.6.5 Set Temperature Optimisation Step (TOS) For optimisation of new PCR protocols very often gradient enabled Thermocyclers are used. The Biometra TRIO offers no gradient function but by the temperature optimisa- tion step (TOS) function programs making use of the three blocks and delivering differ- ent annealing temperatures at the defined step can be created.

  • Page 53: Delete Step

    Biometra TRIO Creating, editing and saving programs Insert Step By using the Insert Step button the Edit Step function (see section "Edit parameter or step" p. 48) is activated. Set the parameters for the new step. Note: The new step is inserted at the selected program step. If for example step 3 is se- lected, the new step will be inserted at step 3.

  • Page 54: Fig. 25 Program Overview Screen

    Creating, editing and saving programs Biometra TRIO location number for these programs will increase by +1 and the new program is saved at the selected location. Fig. 25 Program overview screen There is also the option of selecting a user directory or a program location using the quick select function.

  • Page 55: Fig. 26 Quick Selection Screen

    Biometra TRIO Creating, editing and saving programs Fig. 26 Quick selection screen Before saving the program there is still the possibility of changing the program name. To do this, press the Save as button and enter the new program name using the Bi- ometra TRIO keyboard (see section "Assign program names"...

  • Page 56: Starting, Copying And Deleting Programs

    Starting, Copying and Deleting programs Biometra TRIO Starting, Copying and Deleting programs Start program Note: Programs can only be started by users with the right Start/stop programs acti- vated (see section "Edit user" p. 88). Additionally the right Read all programs defines, if a user is allowed to read all programs or if the access is limited to own programs.

  • Page 57: Fig. 28 Biometra Trio Program Overview Screen With Program Preview

    Biometra TRIO Starting, Copying and Deleting programs Touch the corresponding buttons to select a user directory and a stored program. If the number of user directories or programs is higher than can be maximal displayed in the list, the buttons above and below the user and program list can be used to scroll up and down.

  • Page 58: Copy Program

    Starting, Copying and Deleting programs Biometra TRIO Copy program Note: Own programs can only be copied and saved by users with the right Write/delete own programs activated. Programs of others can be copied and saved with the right Write/delete all programs activated (see section "Edit user" p. 88). Enable a user to edit, copy and delete own programs Enable a user to edit, copy and delete...

  • Page 59: Copy All Programs

    Biometra TRIO Starting, Copying and Deleting programs Fig. 30 Biometra TRIO program overview screen Copy all programs Note: Own programs can only be copied and saved by users with the right Write/delete own programs activated. Programs of others can be copied and saved with the right Write/delete all programs activated (see section "Edit user"...

  • Page 60: Delete Program

    Starting, Copying and Deleting programs Biometra TRIO Note: The programs will be copied into the selected user directory beginning at the first free storage place. Fig. 31 Biometra TRIO program overview screen Delete program Note: Own programs can only be deleted by users with the right Write/delete own pro- grams activated.

  • Page 61: Delete All Programs

    Biometra TRIO Starting, Copying and Deleting programs Fig. 32 Biometra TRIO security query Note: If a program is deleted, all other following programs in the user directory will be moved to a lower storage place. The storage place number for these programs will de- crease by -1.

  • Page 62: Fig. 33 Biometra Trio Security Query

    Starting, Copying and Deleting programs Biometra TRIO Fig. 33 Biometra TRIO security query...

  • Page 63: Running, Pausing, Continuing And Stopping Programs

    Biometra TRIO Running, pausing, continuing and stopping programs Running, pausing, continuing and stopping programs To switch between the view for the sample blocks during run select the number of the sample block in the corresponding screen. Sample block 1 Sample block 2 Sample block 3 Display during run After a program is started (see section "Display during run"...

  • Page 64: Pause Program

    Running, pausing, continuing and stopping programs Biometra TRIO Fig. 35 Biometra TRIO run screen in spreadsheet view. The following parameters are summarized in the headline table:  Program Name  Estimated End  Remaining Time  Sample Block Temperature  Lid Temperature ...

  • Page 65: Continue Program

    Biometra TRIO Running, pausing, continuing and stopping programs Spreadsheet view Graphical view Fig. 36 Biometra TRIO run screen during program pause Note: To press the pause button requires manual interaction. The Biometra TRIO soft- ware also allows to program a pause step (see section "Edit parameter or step" p. 48), then the pause step is automatically started by the instrument.

  • Page 66: Stop Program

    Running, pausing, continuing and stopping programs Biometra TRIO Stop Program Note: Programs can only be stopped by users with the right Start/stop programs acti- vated (see section "Edit user" p. 88). Enable a user to start or stop programs To stop a program press the following button in the Biometra TRIO run screen (see Fig. 34).

  • Page 67: Fig. 38 Biometra Trio Security Query

    Biometra TRIO Running, pausing, continuing and stopping programs Fig. 38 Biometra TRIO security query The Biometra TRIO Thermal Cycler will immediately stop the running sample block(s).

  • Page 68: Protocol Wizard

    Protocol Wizard Biometra TRIO Protocol Wizard The protocol wizard is a helpful tool for automatic creation of PCR programs and primer annealing temperature calculation. To start the protocol wizard press the protocol wiz- ard button in the Biometra TRIO home screen (see section "Home Screen" p. 36). Protocol Wizard 10.1 Select Polymerase The protocol wizard uses polymerase-specific program templates to create PCR pro-...

  • Page 69: Fig. 40 Biometra Trio Screen For Editing Program Templates For Polymerases

    Biometra TRIO Protocol Wizard New Polymerase Edit Polymerase In the next screen (see Fig. 40) touch the corresponding input boxes to enter a name for the polymerase and set parameters for all requested program steps. With two excep- tions for most program steps the temperature and the holdtime must be defined: ...

  • Page 70: Delete Polymerase

    Protocol Wizard Biometra TRIO Fig. 41 Biometra TRIO for saving program templates for polymerases Note: If a new or edited polymerase is to be saved, the first free storage place in the pol- ymerase list is pre-selected. However, the storage place can be modified by using the cursor key function above and below the list.

  • Page 71: Protocol Wizard Parameter Screen

    Biometra TRIO Protocol Wizard 10.4 Protocol Wizard parameter screen For automatic calculation of PCR programs in the protocol wizard parameter screen (see Fig. 43) some parameters have to be set by the user: 1. Touch the corresponding checkbox to select between two step and three step pro- grams.

  • Page 72: Fig. 44 Biometra Trio Protocol Wizard View Parameter Screen

    Protocol Wizard Biometra TRIO Next The next screen lists the all steps of the program that will be created by the software: Fig. 44 Biometra TRIO protocol wizard view parameter screen Press the create program button to enter the Biometra TRIO programming screen (see section "Creating, editing and saving programs"...

  • Page 73: Primer Annealing Temperature Calculator

    Biometra TRIO Protocol Wizard 10.5 Primer Annealing Temperature Calculator Based on the primer sequences the Biometra TRIO protocol wizard can calculate the ). Press the calc Ta button in the Biometra theoretical primer annealing temperature (T TRIO protocol wizard parameter screen (see Fig. 43) to access the primer annealing temperature calculator screen (see Fig.

  • Page 74: Fig. 47 Biometra Trio Screen For Primer Concentration Adjustment

    Protocol Wizard Biometra TRIO Fig. 47 Biometra TRIO screen for primer concentration adjustment Press the edit primer button to toggle to the primer annealing temperature calculator screen (see Fig. 47). Edit primer Or press the calc. primer button to calculate the theoretical primer annealing tempera- ture (T Calc.
  • Page 75 Biometra TRIO Protocol Wizard 1. For each primer the melting temperature is calculated using the following equation according to Wallace et al. = ((w*A + x*T)*2) + ((y*G + z*C)*4) where w, x, y and z are the number of the bases A, T, G and C in the primer se- quence, respectively.

  • Page 76: Tools

    Tools Biometra TRIO Tools The Biometra TRIO software provides the following tools: Settings Define basic instrument settings See section 11.1 User Management Tool for the user management See section 11.2 Documentation Access to stored log files See section 11.3 Extended Self-Test Execute extended self-test See section 11.4 Service Info File (SINF)

  • Page 77: Set Date And Time

    Biometra TRIO Tools Date and Time Set date and time See section "Set Date and Time" p. 77 Automatic user Switch automatic user logout on or off and See section "Automatic user logout" p. 78 time settings logout Beeper Switch beeper on or off See section "Config.

  • Page 78: Automatic User Logout

    Tools Biometra TRIO Fig. 50 Biometra TRIO date and time screen Activate the corresponding input box and use the keypad on the right side of the date and time screen to enter digits. Additionally the following keys might be helpful to fill out the input boxes: Delete single character Delete all characters...

  • Page 79: Config. Beeper

    Biometra TRIO Tools Automatic user logout On Automatic user logout Off If the automatic user logout is switched ON the time after that the user is automatically logged out can be set. You can enter values between 30 minutes and 60 minutes. Fig.

  • Page 80: Network

    Tools Biometra TRIO Fig. 52 Biometra TRIO beeper configuration screen 11.1.4 Network The Network menu item can be used to config. basic settings or show a list of network users. Press the corresponding button: Network settings Network user Note: The network settings can only be configured by users with the right System con- Network settings figuration activated (see section "Edit user"...

  • Page 81: Fig. 53 Network Settings Screen, Ip Settings Via Static

    Biometra TRIO Tools Go to the next input field Fig. 53 Network settings screen, IP settings via static Fig. 54 Network settings screen, IP settings via DHCP Note: The network cable must be at least performance class Cat 5e and the cable config- uration has to be STP.

  • Page 82: Display Brightness

    Tools Biometra TRIO  Live monitoring of PCR runs on network-integrated thermal cyclers- Starting and stopping PCR protocols are stored on the thermal cycler  Notification system for monitoring status changes on selected blocks of a thermal cy- cler  Store PCR protocols on the terminal. This can be stored on a PC using a USB stick ...

  • Page 83: Touch Screen Calibration

    Biometra TRIO Tools 11.1.6 Touch screen calibration Note: The touch screen can only be calibrated by users with the right System configura- tion activated (see section "Edit user" p. 88). Enable a user to config. the system configuration To calibrate the touch screen follow the instructions and touch the screen at the center of the displayed circle.

  • Page 84: User Management

    Tools Biometra TRIO Fig. 57 Biometra TRIO security query By using the factory settings tool all user accounts and programs will be deleted! Note: Use a backup file (see section "Backup" p. 98) to restore the system. 11.2 User Management The Biometra TRIO thermal cycler manages up to 90 individual user accounts.

  • Page 85: Fig. 58 Biometra Trio User Management Screen

    Biometra TRIO Tools Fig. 58 Biometra TRIO user management screen In the next screen activate the corresponding checkbox to config. the Biometra TRIO user management: Fig. 59 Biometra TRIO config. user management screen If the user management is switched off, all new users have all rights and can access all software functions.

  • Page 86: Create User

    Tools Biometra TRIO Administrator User with generic User with limited rights rights System configuration Edit user Create user Delete user Write/delete all programs Write/delete own programs Read all programs Start/stop programs # An administrator can create users of all groups and modify their rights settings. A user with generic rights is only allowed to create users with generic or limited rights but no administrator.

  • Page 87: Fig. 60 Biometra Trio Create User Screen

    Biometra TRIO Tools Enable a user to create a new user An administrator can create users of all groups and modify their rights settings. A user with generic rights is only allowed to create users with generic or limited rights but no administrator.

  • Page 88: Edit User

    Tools Biometra TRIO 11.2.2 Edit user Note: User rights can only be edited by users with the right Edit User activated. Enable a user to edit user rights To edit the user properties select a user account in the user management screen and press the button: Edit user account In the next screen, the user data and the language settings can be changed.

  • Page 89: Fig. 62 Biometra Trio User Properties Screen

    Biometra TRIO Tools rights are highlighted blue, deactivated rights are shown in grey. The settings for each user will be stored by the Biometra TRIO software and are shown in the Biometra TRIO user management screen (see section "User Management" p. 84). Fig.

  • Page 90: Delete User

    Tools Biometra TRIO User data 11.2.3 Delete user Note: User accounts can only be deleted by users with the right Delete User activated (see section "Edit user" p. 88). Enable a user to delete a user To delete a user select the user account in the user management screen (see section "User Management"...

  • Page 91: Run-Logfile

    Biometra TRIO Tools Fig. 64 Biometra TRIO documentation screen Press the corresponding button to access the desired function. 11.3.1 Run-Logfile The Biometra TRIO Thermal Cycler lists up to 6 Run-Logfiles for the latest programs. Fig. 65 Biometra TRIO Run-Logfile overview screen The Run-Logfiles are sorted by date and time.

  • Page 92: Fig. 66 Biometra Trio Run-Logfile Screen

    Tools Biometra TRIO In the Run-Logfile screen the following information is listed:  Run-Logfile name  Serial number Cycler  Log time and date  Block type  Start and end time of the program run  Block serial number ...

  • Page 93: Power-On-Logfile

    Biometra TRIO Tools Fig. 67 Biometra TRIO View program screen Use the following buttons to toggle between the overview screen, view Run-Logfile screen and view program screen. Overview Run-Logfiles View Run-Logfile View Program To export Run-Logfiles to USB press: Save all Run-Logfiles to USB Select a logfile from the list and press the following button in the Run-Logfile overview screen (see Fig.

  • Page 94: Extended Self-Test-Logfile

    Tools Biometra TRIO  Last Power-down by date and time  Table with failure messages by number, date, time, error code and message Fig. 68 Biometra TRIO Power-On-Logfile screen 11.3.3 Extended Self-Test-Logfile During the Extended Self-Test the Biometra TRIO Thermal Cycler checks several func- tions and components like cooler, thermal tracking, heating and cooling rate, refrigera- tion, heated lid and regulation (see fig.

  • Page 95: Error-Logfile

    Biometra TRIO Tools 11.3.4 Error-Logfile The Biometra TRIO Thermal Cycler records all errors by time and date. The latest error is shown in position in the Error Log table. Additionally the error code and error message are listed. Fig. 70 Biometra TRIO Error-Logfile screen 11.4 Extended Self-Test NOTICE...

  • Page 96: Fig. 71 How To Load Plastic Ware For Extended Self-Test

    Tools Biometra TRIO Test Function Heating and Checks, if the specified average heating and cooling rate is reached. cooling rate Refrigeration The cooling sink and fans are checked to work properly together. Gradient Checks, if the sample block reaches the set gradient temperatures. Heated lid Tests, if the heated lid reaches the set temperature and if it holds the temperature for a longer time.

  • Page 97: Fig. 72 Biometra Trio Extended Self-Test Screen

    Biometra TRIO Tools Fig. 72 Biometra TRIO Extended Self-Test screen During the test the software provides a progress overview. If a test is already passed the result “Failed” or “Passed” is displayed, for the currently performed test “Running” is shown and all other tests are not finished yet. Note: The Extended Self-Test will take approximately 30 minutes to finish and the in- strument is blocked, no other program can be started while the test is running.

  • Page 98: Service Info File (Sinf)

    Tools Biometra TRIO 11.5 Service Info File (SINF) The Service Info File is a helpful tool for remote failure diagnosis by the Biometra service department. Fig. 74 Biometra TRIO create Service Info File screen To create a Service Info File connect a USB stick to the Biometra TRIO Thermal Cycler and press: Info file to USB The Service Info File can be send by e-mail to the Biometra service department.

  • Page 99: Cycler Info

    Biometra TRIO Tools Fig. 75 Biometra TRIO backup file screen To load a backup file select a backup from the list and press: Load backup file Backup files that are no longer used can be deleted by using the functions Delete all Backup Files Delete Backup File 11.7 Cycler Info...

  • Page 100: Contact

    Tools Biometra TRIO System/Cycler Info Fig. 76 Biometra TRIO cycler info screen 11.8 Contact Shows contact details for the Biometra service department. Fig. 77 Biometra TRIO contact details screen...

  • Page 101: Adaptation Of Programs

    Biometra TRIO Adaptation of programs Adaptation of programs If programs are exchanged between different thermal cycler models or of the sample block is exchanged programs might need to be adapted prior to start or during editing. The following table provides an overview for possible adaptations: Cause Example Adjustment...

  • Page 102: Short Manual

    Short Manual Biometra TRIO Short Manual WARNING Before initial operation check the correct operating voltage is set on the voltage selec- tion switch on the underside of the device. Do not open the housing! Danger of electric shock! CAUTION The sample block and the heated lid can reach high temperatures during operation. Both the sample block and the heated lid can burn you.
  • Page 103 Biometra TRIO Short Manual  Press Quick start for the user specific quick start of programs  Press Programs to edit, save, copy or run programs  Press Incubate to incubate the sample block at a constant temperature  Press Tools to access the system settings, user management, documentation, backup tool or self-test functions ...
  • Page 104 Short Manual Biometra TRIO  Define the heated lid status and temperature. Touch the input box “Lid” and switch the heated lid on or off. If the heated lid is switched on enter a temperature between 30 °C and 110 °C. ...
  • Page 105 Biometra TRIO Short Manual  To insert a step select a program step in the programming screen and press Insert. The edit step screen opens. Enter the desired parameters and confirm the settings by pressing the button  To delete a step select a program step and press Delete. In the security query confirm to delete the step by pressing the button ...
  • Page 106 Short Manual Biometra TRIO Spreadsheet view Graphical view  To pause the active program press the button Pause. The color of the highlighted step changes to blue and in the field “Step” the message Pause is shown.  To continue a paused program press the button Continue. ...

  • Page 107: Fault Removal

    Biometra TRIO Fault removal Fault removal 14.1 Forgotten password Passwords can be changed by the systems administrator(s) and by users with the acti- vated right Edit User (see section "Edit user" p. 88). If the administrator Admin has for- gotten the password and no one else has the right Edit user use the function Factory settings (see section "Factory settings"...

  • Page 108: Mirror Programs To Several Thermal Cyclers

    Fault removal Biometra TRIO 14.5 Mirror programs to several thermal cyclers Using the backup function, complete thermal cyclers can be mirrored. Copy complete us- ers (via user management) and their programs from one thermal cycler to the other without having to create each individual user or program. The prerequisite is that both cyclers have the same software version (see section 11.7).

  • Page 109: Maintenance And Repair

    Biometra TRIO Maintenance and Repair Maintenance and Repair 15.1 Factory calibration This instrument is supplied fully calibrated by the manufacturer before delivery, using equipment traceable to national standards. The Biometra Quality Management-System is certified by BSI for BS EN ISO 9001:2015 and the used measurement system is tested by an accredited testing laboratory in accordance with EN ISO 17025 at prescribed inter- vals and gets regularly calibrated.

  • Page 110: Disinfecting The Device

    Maintenance and Repair Biometra TRIO  Only wipe the housing of the Biometra TRIO with a soft clean cloth which may be slightly wetted with a commercial neutral detergent, if necessary.  Clean the ventilation slits on the underside and at the rear of the device with a vac- uum cleaner.

  • Page 111: Service

    Biometra TRIO Service Service Should you have any problems with this unit, please contact our service department or your local Biometra dealer. Note: If you would like to send the unit back to us, please read the following return in- structions.

  • Page 112: Packing Of The Biometra Trio Thermal Cycler

    Service Biometra TRIO 16.2 Packing of the Biometra TRIO Thermal Cycler Biometra uses an extra designed packaging system with inserts of foamed material. NOTICE The Thermal Cycler is only protected from transport damage if the original transport box is used and the packing instructions are followed. Biometra is not responsible for transport damage by improper packing.

  • Page 113: Disposal

    Biometra TRIO Disposal Disposal The operator of the Biometra TRIO must dispose of any waste materials that occur dur- ing measurements (sample materials) in accordance with statutory and local regula- tions. At the end of its service life, the Biometra TRIO and all its electronic components must be disposed of as electronic waste in accordance with valid regulations.

  • Page 114: Technical Specifications

    Technical specifications Biometra TRIO Technical specifications Order Number 846-x-070-720 846-x-070-723 846-x-070-724 Name Biometra TRIO 30 Biometra TRIO 48 Biometra TRIO combi Capacity 3 blocks for 30 x 0.5ml 3 blocks for 48 x 0.2ml 3 combi blocks for 18 x 0.5ml tubes each tubes tubes**...
  • Page 115 Biometra TRIO Technical specifications Order Number 846-x-070-720 846-x-070-723 846-x-070-724 Interfaces USB A, Ethernet Working conditions 15 °C to 35 °C, 70 % air humidity, max 2000 m NN Dimensions (WxDxH) 30 cm x 41 cm x 25 cm Weight 17.3 kg * Measured inside the block ** Capacity increases to 35 x 0.5 ml tubes by use of small cap tubes 1 The network cable must be at least performance class Cat 5e and the cable configuration has to be...

  • Page 116: Declaration Of Conformity

    Declaration of Conformity Biometra TRIO Declaration of Conformity...

  • Page 117: Index

    Biometra TRIO Index Index Auto restart 107 Administrator 36 Backup 98 forgotten 107 Block status 37 User 86 Care 109 Personnel 12 Clean the housing 110 Power On Self-Test 34 Commands 28 Program Contact 100 Continue 65 Decontamination 110 Copy 58 Disinfection 110 Copy all 59 Display...
  • Page 118 Index Biometra TRIO Quick start function 38 Technical data 114 Safety instructions 11 Tools 76 Settings 76 Cycler Info 99 Automatic logout 78 Self-Test 95 Beeper 79 Service Info File 98 Date/time 77 User management 84 Display 82 Create user 86 Network 80 Delete user 90 Reset factory settings 83...

This manual is also suitable for:

846-5-070-720846-4-070-720846-2-070-720846-2-070-723846-2-070-724

Table of Contents