Example: Indirect Detection Using The Plating Bath Buffer - Agilent Technologies 7100 Technical Note

Example: Indirect detection using the Plating Bath Buffer

A test method can be created using the Plating Bath Buffer for testing the
indirect detection functions. The following reagents and parameters can be
used:
Capillary:
Electrolyte:
Home vials:
Sample:
Voltage:
Temperature:
Stop time:
Preconditioning:
Injection:
DAD:
Comparing the reversed wavelength the peaks can be shown as positive or
negative peaks.
Signal A: 350 nm/BW 20 nm, Ref 275 nm/BW 10 nm
Signal B: 275 nm/BW 10 nm, Ref 350 nm/BW 20 nm
Figure 29
Agilent 7100 CE in Empower Environment
Fused silica capillary 50 µm ID, 80.5 cm total (72 cm effective)
(G1600-62211)
Plating Bath Buffer (Agilent: 5064-8236)
1 and 2 (filled with electrolyte solution)
Suitable Plating Bath Mixture (laboratory made)
-30 kV
25°C
15 min
240 s (from electrolyte vial 3 into waste vial)
1 Injection vial to outlet vial: 50 mbar for 10 s
2 Post-injection: 50 mbar for 5 s (from inlet vial to outlet vial)
Signal A: 350 nm (BW 20 nm), reference: 275 nm (BW 10 nm)
Signal B: 275 nm (BW 10 nm), reference: 350 nm (BW 20 nm)
Peak width: >0.025 min (10 Hz)
Timetable: 1.0 min Balance
Indirect detection
APPENDIX - Examples
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