Example: Multiple Injections Using The Iq Kit For Ce - Agilent Technologies 7100 Technical Note

Example: Multiple injections using the IQ Kit for CE

For testing multiple injections in one sequence, the instrument method and
the experimental setup described in section Example: Single test run can be
used. See the sequence table (Figure 27) and the overlay of the resulting
electropherograms (Figure 28).
A set of six injections was used to calculate system precision. The peak
areas and relative standard deviations (Excel: STDEV) for the areas,
migration times and the corrected areas are summarized in the following
table.
Table 2
Injection 1
Injection 2
Injection 3
Injection 4
Injection 5
Injection 6
Average
Deviation STDEV:
Sixfold sample injection
Figure 27
Agilent 7100 CE in Empower Environment
Peak areas and relative standard deviations
Area
50049
50547
50189
49767
49187
49579
49886
0.96 %
Sequence table for multiple injections
t [min] t [s]
m m
2.730 163.80
2.723 163.38
2.717 163.02
2.711 162.66
2.706 162.36
2.702 162.12
2.715 162.89
0.39 % 0.39 %
APPENDIX - Examples
corr. Area (area/t [s])
m
3.05549E+02
3.09383E+02
3.07870E+02
3.05957E+02
3.02950E+02
3.05817E+02
3.06254E+02
0.72 %
39